The effects of antimicrobials and lipopolysaccharide on acute immune responsivity in pubertal male and female CD1 mice

The acute effects of antimicrobials and lipopolysaccharide on the cellular mechanisms associated with neurodegeneration in pubertal male and female CD1 mice

“…At five weeks of age, mice were administered 200 μL of mixed broad-spectrum antimicrobial solution or water through gavage twice daily for 7 day as described in ( Esposito et al, 2022 ). Briefly, the antimicrobial solution was made fresh daily and contained 15 mg/mL of ampicillin (No.…”

Enduring sex-dependent implications of pubertal stress on the gut-brain axis and mental health

“…For example, exposure to Kefir containing active bacterial culture ( L. lactis, L. cremoris, L. diacetylactis, L. acid-ophilus ), lactic yeasts and skim milk powder one-week prior to and one-week following pubertal LPS treatment reduced sickness behavior ( Murray et al, 2019 ). Another study using a probiotic mixture containing Lactobacillus helveticus and Bifidobacterium longum showed decreased cytokine concentrations in the blood of CD-1 mice ( Esposito et al, 2022 ). These findings hold significant implications, as elevated levels of inflammatory cytokines are known to be associated with an increased risk of neuropsychiatric disorders and depression ( Felger and Lotrich, 2013 ).…”

Environmental Enrichment Cage for Laboratory Mice: A Downloadable Alternative

“…Selecting outbred mice increases genetic diversity and offers greater external validity to our experimental findings (Voelkl et al., 2020). These mice elicit acute and enduring negative changes in response to pubertal stress (Esposito, Gandelman et al., 2022; Esposito, Kearns et al., 2022; Murack et al., 2021, 2023; Murray et al., 2019, 2020; Smith, Murray, Chandrasegaram et al., 2021; Smith, Murray, Gregory et al., 2021). All experiments must receive ethics approval from institutional animal care and ethics committee before proceeding.…”