The acute effects of antimicrobials and lipopolysaccharide on the cellular mechanisms associated with neurodegeneration in pubertal male and female CD1 mice

Esposito, Pasquale; Gandelman, Michelle; Rodriguez, Cloudia; Liang, Jacky; Ismail, Nafissa

doi:10.1016/j.bbih.2022.100543

Cited by 3 publications

(1 citation statement)

References 88 publications

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“…Selecting outbred mice increases genetic diversity and offers greater external validity to our experimental findings (Voelkl et al., 2020). These mice elicit acute and enduring negative changes in response to pubertal stress (Esposito, Gandelman et al., 2022; Esposito, Kearns et al., 2022; Murack et al., 2021, 2023; Murray et al., 2019, 2020; Smith, Murray, Chandrasegaram et al., 2021; Smith, Murray, Gregory et al., 2021). All experiments must receive ethics approval from institutional animal care and ethics committee before proceeding.…”

Section: Commentarymentioning

confidence: 99%

Environmental Enrichment Cage for Laboratory Mice: A Downloadable Alternative

Smith,

Murack,

Sharani

et al. 2024

Current Protocols

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Environmentally enriched housing (EE) provides a stimulating and species‐typical environment that enhances brain plasticity and cognition, while reducing disease severity in laboratory animals. However, standardizing EE protocols has been challenging due to issues such as variability, high pricing, or limited laboratory space. To address these challenges, we present a replicable and cost‐efficient cage protocol that is accessible to researchers with limited resources and space constraints. The protocol is designed to provide a stimulating and species‐typical environment for the animals. It incorporates elements such as social interaction, physical exercise, cognitive stimulation, manipulable objects, environmental variability, and sensory stimulation. As evidenced in our previous studies using our protocol, users can expect to observe similar neuroplastic and health‐wise benefits that accompany EE experimental paradigms. These included straightforward step‐by‐step guide, which allows for construction of functional EE cages in under 8 hr. Basic knowledge of 3D printing and laser cutting is required, but no advanced skills are necessary. The protocol enables researchers to create stimulating and replicable environments that promote animal welfare, enhance brain plasticity, and yield valuable experimental results for low cost. © 2024 Wiley Periodicals LLC.Basic Protocol: An effective and cost‐efficient environmental enrichment cage designed to encourage standardization amongst laboratory protocols.

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