The effects of amino acids and fatty acids on the disease resistance of Epinephelus fuscoguttatus in response to Vibrio vulnificus infection

Nurdalila, A’wani Aziz; Natnan, Maya Erna; Baharum, Syarul Nataqain

doi:10.1007/s13205-020-02543-4

Cited by 11 publications

(11 citation statements)

References 52 publications

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“…V. vulnificus was primarily obtained from infected liver, spleen, and kidney via fish dissection [ 22 , 28 ]. The bacterial strain was identified using a molecular approach.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Different Dietary Fatty Acids Supplement on the Immune Response of Hybrid Grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) Challenged with Vibrio vulnificus

Natnan

Low

Chong

et al. 2022

Biology

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Aquaculture has been expanding in Malaysia due to the increased demand for fish products. In addition, aquaculture faces challenges in maintaining feed suitability in support of the global growth of fish production. Therefore, improvements in diet formulation are necessary to achieve the optimal requirements and attain a desirable growth efficiency and health performance in fish. Seven weeks of study were conducted to compare the equal amounts of different fatty acids (2%) (oleic acid, stearic acid, palmitic acid, and behenic acid) on the survival, the growth, and the immune response of hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) against V. vulnificus. After six weeks of the feeding trial, fish were challenged with V. vulnificus for 30 min before continuing on the same feeding regime for the next seven days (post-bacterial challenge). Fish supplemented with dietary oleic acid showed significantly (p < 0.05) enhanced immune responses, i.e., lysozyme, respiratory burst, and phagocytic activities compared to the control diet group for both pre-and post-bacterial challenges. Following the Vibrio challenge, no significant effects of supplemented fatty acid diets on survival rate were observed, although dietary oleic acid demonstrated the highest 63.3% survival rate compared to only 43.3% of the control diet group. In addition, there were no significant effects (p > 0.05) on specific growth rate (SGR), white blood cell (WBC), and red blood cell (RBC) counts among all experimental diets. The results from this study suggest that among the tested dietary fatty acids, the oleic acid diet showed promising results in the form of elevated immune responses and increased disease resistance of the hybrid grouper fingerlings challenged with V. vulnificus.

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“…V. vulnificus was primarily obtained from infected liver, spleen, and kidney via fish dissection [ 22 , 28 ]. The bacterial strain was identified using a molecular approach.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Different Dietary Fatty Acids Supplement on the Immune Response of Hybrid Grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) Challenged with Vibrio vulnificus

Natnan

Low

Chong

et al. 2022

Biology

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“…These results highlight that, after exposure to an infectious pathogen, crucial metabolites are upregulated and act as biomarkers of fish's immune response. Additionally, our previous study on brown marble grouper (E. fuscoguttatus) infected with V. vulnificus showed higher production of metabolites from omega-9 fatty acids such as palmitoleic acid, oleic acid, 6,9-octadecenoic acid, cis-erucic acid, 8,11-eicosadienoic acid and 5,8,11-eicosatrienoic using a GC-MS approach (Nurdalila et al, 2019(Nurdalila et al, , 2020. Based on this result, metabolomics profiling has the potential to investigate fish metabolic responses toward disease infection.…”

Section: Metabolomicsmentioning

confidence: 99%

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

et al. 2021

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Nowadays, fish production through aquaculture and wild capture is escalating due to the higher fish protein demand for human consumption. Thus, the needs for fish products to be improved in scale and quality are becoming increasingly important to meet the nutrition requirements. The negative impacts caused by pathogens infection both in hatchery and grow-out ponds, have resulted in low aquaculture productivity and economic losses for the farmers. Therefore, a systematic study exploring relevant omics datasets through a systems biology approach will enable scientist to describe the complexity and characteristics of interactions in the host-pathogen network, leading to identifying new targets or biomarkers for diseases infection caused by bacteria, viruses, and parasites. The use of different approaches and emerging technologies as a powerful tool in obtaining a new knowledge for sustainable aquaculture production has been increasingly applied by many researchers over the years. In this paper, several omics approaches used to assess the fish immune response were reviewed. A brief description is provided for the omics approaches applicable to aquacultures, such as genomics, transcriptomics, proteomics, and metabolomics. Each tool used are contributes to develop better treatment and prevention strategies to overcome aquaculture disease due to the microbial infections. In genomics and transcriptomics analysis, their parallel approach involving gene expression studies play important role in identifying immune-related genes of the host, response to infectious diseases. While for proteomics and metabolomics approaches, these omics will provide information on the biomarker discovery and understanding the immune-related biosynthetic pathway during pathogen infection. Additionally, examples of the integrative analysis of multi-omics tools required for researchers to better understand the biological systems of aquatic animals especially fish, will also be provided.

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“…A study by Du et al [ 97 ] reported on the effects of l-leucine-induced metabolome to eliminate Streptococcus iniae in tilapias, exploring the metabolomics approach’s potential in infectious diseases. Other studies on the metabolome changes during bacterial infection also have been conducted on several fish species such as tilapia [ 133 ], Atlantic salmon [ 40 , 134 ], crucian carp [ 135 ], and grouper [ 136 ]. A recent study by Low et al [ 49 ], discussed the limitations and the challenges of metabolomics applications in fish disease studies while emphasizing the impacts of infectious fish diseases and the possibility of enhancing disease resistance in fish using metabolomics.…”

Section: Multi-omics Perspective On Infectious Fish Diseases Studiesmentioning

confidence: 99%

“…In another study [ 136 ], the high concentration of amino acid such as valine and leucine detected in the susceptible group of groupers infected with vibriosis is related to the activation of anti-bacterial infection pathways that involves valine, leucine, and isoleucine metabolism. Identification of these metabolites (valine and leucine) and their specific functions in inhibiting or escalating the immune response has enabled multiple other research to be conducted in order to improve the current problems in fish disease.…”

Section: Multi-omics Perspective On Infectious Fish Diseases Studiesmentioning

confidence: 99%

“…While hypoxia is not an infectious disease, the lack of oxygen in the fish system is seen to achieve a similar stress response in the fish when infected with pathogens, according to the results of a study conducted by Lardon et al [ 140 ]. Since amino acids are vital to regulating key metabolic pathways related to maintenance and immune responses, identifying specific amino acids unique to certain biological pathways is essential to discover biologically active metabolites [ 136 , 137 ]. In another study, the effects of waterborne chlorpyrifos in freshwater carp, Cyprinus carpio had caused the increased biosynthesis of valine, leucine, and isoleucine for oxidative/ stress metabolism, while the increased of, alanine and lactate pattern suggests that the subsequent metabolic pathway was inhibited.…”

Section: Multi-omics Perspective On Infectious Fish Diseases Studiesmentioning

confidence: 99%

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Omics Strategies in Current Advancements of Infectious Fish Disease Management

Natnan

Mayalvanan

Jazamuddin

et al. 2021

Biology

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Aquaculture is an important industry globally as it remains one of the significant alternatives of animal protein source supplies for humankind. Yet, the progression of this industry is being dampened by the increasing rate of fish mortality, mainly the outbreak of infectious diseases. Consequently, the regress in aquaculture ultimately results in the economy of multiple countries being affected due to the decline of product yields and marketability. By 2025, aquaculture is expected to contribute approximately 57% of fish consumption worldwide. Without a strategic approach to curb infectious diseases, the increasing demands of the aquaculture industry may not be sustainable and hence contributing to the over-fishing of wild fish. Recently, a new holistic approach that utilizes multi-omics platforms including transcriptomics, proteomics, and metabolomics is unraveling the intricate molecular mechanisms of host-pathogen interaction. This approach aims to provide a better understanding of how to improve the resistance of host species. However, no comprehensive review has been published on multi-omics strategies in deciphering fish disease etiology and molecular regulation. Most publications have only covered particular omics and no constructive reviews on various omics findings across fish species, particularly on their immune systems, have been described elsewhere. Our previous publication reviewed the integration of omics application for understanding the mechanism of fish immune response due to microbial infection. Hence, this review provides a thorough compilation of current advancements in omics strategies for fish disease management in the aquaculture industry. The discovery of biomarkers in various fish diseases and their potential advancement to complement the recent progress in combatting fish disease is also discussed in this review.

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The effects of amino acids and fatty acids on the disease resistance of Epinephelus fuscoguttatus in response to Vibrio vulnificus infection

Cited by 11 publications

References 52 publications

Comparison of Different Dietary Fatty Acids Supplement on the Immune Response of Hybrid Grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) Challenged with Vibrio vulnificus

Comparison of Different Dietary Fatty Acids Supplement on the Immune Response of Hybrid Grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) Challenged with Vibrio vulnificus

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

Omics Strategies in Current Advancements of Infectious Fish Disease Management

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