The effects of aging on satellite cells in skeletal muscles of mice and rats

Abstract

Myosatellite cells were examined and quantified at the fine structural level of resolution during aging of skeletal muscles in mice and rats. Satellite cells in the soleus and gastrocnemius muscles of animals between eight and 30 months of age appeared, according to morphological criteria, metabolically less active than those examined in immature muscles. In the soleus muscle of the mouse, satellite cells decreased in number from 4.6% at eight months of age to 2.4% at 30 months. This decrease appeared to be due to the passage of some satellite cells into the interstitial space as a result of the formation of external lamina material around the entire satellite cell surface.

Skeletal myogenesis by human embryonic stem cells

“…In healthy, mature muscle, a satellite cell pool is maintained [23]. These cells are located next to the muscle fiber and under the basal lamina [1].…”

Adeno-associated virus-mediated gene transfer of the heart/muscle adenine nucleotide translocator (ANT) in mouse

“…This may reflect the broader distribution of the vector upon injection into neonatal tissue as well as the initial infection of not only muscle fibers but also a larger number of muscle precursor cells present in neonatal tissue. 19 These transduced precursor cells could subsequently proliferate and differentiate into mature fibers expressing the transgene.…”

Investigating the Influence of Extracellular Matrix and Glycolytic Metabolism on Muscle Stem Cell Migration on Their Native Fiber Environment

“…Satellite cells in resting muscle exist in a quiescent state exemplified by the relative lack of cytoplasm and its associated organelles in relation to the size of the nucleus [18]. The metabolic activity of the mitochondria in quiescent satellite cells is thought to be low due to its inactive state and to minimize the generation of reactive oxygen species (ROS) that would otherwise impact on the control of cell proliferation, survival, stem cell self-renewal as well as influence the molecular pathways that regulate differentiation.…”