Skeletal myogenesis by human embryonic stem cells

Zheng, Jun; Wang, Yi; Karandikar, Aditi; Wang, Qian; Gai, Hongwei; Liu, Ai Lian; Chao, Peng; Sheng, Hua

doi:10.1038/sj.cr.7310080

Cited by 71 publications

(58 citation statements)

References 29 publications

(38 reference statements)

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“…Skeletal muscle satellite cells can be cultured in vitro from human muscle biopsy tissues as undifferentiated, mononucleated cells, and their proliferative capacity makes them an ideal model for in vitro infection studies of skeletal muscle. Desmin and CD56 are both considered reliable markers for muscle satellite cells among cells cultured from skeletal muscle (Ozden et al, 2007;Stewart et al, 2003;Zheng et al, 2006). Fibroblasts, which co-propagate in cell culture, do not express CD56 (Allen et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells

Warke

Becerra

Zawadzka³

et al. 2008

Journal of General Virology

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Dengue virus (DENV) is a mosquito-borne flavivirus that causes an acute febrile disease in humans, characterized by musculoskeletal pain, headache, rash and leukopenia. The cause of myalgia during DENV infection is still unknown. To determine whether DENV can infect primary muscle cells, human muscle satellite cells were exposed to DENV in vitro. The results demonstrated for the first time high-efficiency infection and replication of DENV in human primary muscle satellite cells. Changes in global gene expression were also examined in these cells following DENV infection using Affymetrix GeneChip analysis. The differentially regulated genes belonged to two main functional categories: cell growth and development, and antiviral type I interferon (IFN) response genes. Increased expression of the type I IFN response genes for tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), melanoma-derived antigen 5 (MDA-5), IFN-c-inducible protein 10 (IP-10), galectin 3 soluble binding protein (LGals3BP) and IFN response factor 7 (IRF7) was confirmed by quantitative RT-PCR. Furthermore, higher levels of cell-surface-bound intracellular adhesion molecule-1 (ICAM-1) and soluble ICAM-1 in the cellculture medium were detected following DENV infection. However, DENV infection impaired the ability of the infected cells in the culture medium to upregulate cell-surface expression of MHC I molecules, suggesting a possible mechanism of immune evasion by DENV. The findings of this study warrant further clinical research to identify whether muscle cells are targets for DENV infection during the acute stage of the disease in vivo. INTRODUCTIONDengue is an increasingly troublesome, mosquito-borne viral disease that is highly prevalent in both tropical and subtropical regions of the world. Annually, 50-100 million cases of dengue fever (DF) and 250 000 cases of dengue haemorrhagic fever (DHF) occur worldwide, as determined by the World Health Organization (Nathan & DayalDrager, 2006;Petersen & Marfin, 2005). Infection with any one of the four serotypes of DENV (DENV1-4) can cause DF or DHF. The general mechanisms that control virus replication and are involved in disease pathogenesis are not well understood in this complex, acute illness.Monocytes, dendritic cells and B cells are known to be susceptible to DENV in vivo (Halstead, 1989;King et al., 1999;Lin et al., 2002;Marovich et al., 2001;Wu et al., 2000), whilst DENV antigen has been detected in sinusoidal endothelial cells (Jessie et al., 2004) and hepatocytes (de Macedo et al., 2006;Rosen et al., 1999) in autopsy studies. In addition, a variety of primary human cells, including mast cells (King et al., 2002), endothelial et al., 2007) and hepatocytes (Suksanpaisan et al., 2007), can serve as hosts for DENV in vitro.Symptomatically, patients with dengue often present with general muscle affection as well as severe, persisting myalgia, headache and rash (Chaturvedi et al., 1970;Halstead, 1966). Higher serum levels of creatine phosphokinase (CPK), which is specifically produ...

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Section: Introductionmentioning

confidence: 99%

Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells

Warke

Becerra

Zawadzka³

et al. 2008

Journal of General Virology

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“…Here, we demonstrate a physical mechanism by which alignment information from a geometric boundary can propagate over long distances to guide the organization of muscle tissue. Understanding the details of how muscle tissue forms from individual cells will have important implications for future developmental biology, regenerative medicine and systems theory (Mahmud et al, 2009;Parrish and Edelstein-Keshet, 1999;Zheng et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Cellular self-organization by autocatalytic alignment feedback

Junkin

Leung

Whitman

et al. 2011

Journal of Cell Science

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SummaryMyoblasts aggregate, differentiate and fuse to form skeletal muscle during both embryogenesis and tissue regeneration. For proper muscle function, long-range self-organization of myoblasts is required to create organized muscle architecture globally aligned to neighboring tissue. However, how the cells process geometric information over distances considerably longer than individual cells to self-organize into well-ordered, aligned and multinucleated myofibers remains a central question in developmental biology and regenerative medicine. Using plasma lithography micropatterning to create spatial cues for cell guidance, we show a physical mechanism by which orientation information can propagate for a long distance from a geometric boundary to guide development of muscle tissue. This long-range alignment occurs only in differentiating myoblasts, but not in non-fusing myoblasts perturbed by microfluidic disturbances or other non-fusing cell types. Computational cellular automata analysis of the spatiotemporal evolution of the self-organization process reveals that myogenic fusion in conjunction with rotational inertia functions in a self-reinforcing manner to enhance long-range propagation of alignment information. With this autocatalytic alignment feedback, well-ordered alignment of muscle could reinforce existing orientations and help promote proper arrangement with neighboring tissue and overall organization. Such physical self-enhancement might represent a fundamental mechanism for long-range pattern formation during tissue morphogenesis.

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“…While it has been shown that the early stages of myogenesis occur in EBs [104,105], generating cells with the desired regenerative capacity for tissue engineering purposes have proved elusive. It has been postulated that this is because in vitro cultures do not form the notochord or neural tube, and so the key signaling events that are orchestrated by these structures in vivo cannot occur [106].…”

Section: Pscs and Myogenic Differentiationmentioning

confidence: 99%

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

2018

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The rise in the incidence of musculoskeletal diseases is attributed to an increasing ageing population. The debilitating effects of musculoskeletal diseases, coupled with a lack of effective therapies, contribute to huge financial strains on healthcare systems. The focus of regenerative medicine has shifted to pluripotent stem cells (PSCs), namely, human embryonic stem cells and human-induced PSCs, due to the limited success of adult stem cell-based interventions. PSCs constitute a valuable cell source for musculoskeletal regeneration due to their capacity for unlimited self-renewal, ability to differentiate into all cell lineages of the three germ layers and perceived immunoprivileged characteristics. This review summarizes methods for chondrogenic, osteogenic, myogenic and adipogenic differentiation of PSCs and their potential for therapeutic applications.

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Skeletal myogenesis by human embryonic stem cells

Cited by 71 publications

References 29 publications

Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells

Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells

Cellular self-organization by autocatalytic alignment feedback

Augmentation of Musculoskeletal Regeneration: Role for Pluripotent Stem Cells

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