The Effects of Acetic Acid Concentration and Extraction Temperature on Physical and Chemical Properties of Pigskin Gelatin

Sompie, Meity; Surtijono, S.E.; Pontoh, Julius; Lontaan, Nova

doi:10.1016/j.profoo.2015.01.042

Cited by 44 publications

(29 citation statements)

References 20 publications

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“…At the concentration of 1.2 M and 1.5 M the protein content did not further increase because the optimum concentration has been met. The increase in the acid concentration would (Niu et al, 2013;Sompie et al, 2015). The gelatin extract from buffalo hide had the ash content approximately from 0.53 ± 0.04 to 1.23 ± 0.09%.…”

Section: Chemical Composition Of Hide and Gelatinmentioning

confidence: 97%

“…The acid treatment brokedown both the intra-and intermolecular collagen crosslink so that the collagen structure became more open and weak. The water holding capacity lessened and increased the quantity of water evaporation during drying process (Sompie et al 2015). The moisture of buffalo hide gelatin ranged from 7.05 ± 0.07 to 8.92 ± 0.06%.…”

Section: Chemical Composition Of Hide and Gelatinmentioning

confidence: 99%

“…Said et al (2011) examined the gelatin of goat skin, the protein content were in the range of 89.37 ±1.19% -90.74 ± 1.82%. Sompie et al (2015) reported that gelatin from pig skin, were in the range of 86.03 -89.22%. This result was also similar to commercial gelatin approximately at 89.63% (Pranoto et al, 2006).…”

Section: Chemical Composition Of Hide and Gelatinmentioning

confidence: 99%

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The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Mulyani

Setyabudi

Pranoto

et al. 2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKTujuan penelitian adalah untuk mengidentifikasi variasi konsentrasi asam klorida terhadap yield dan sifat fisikokimia gelatin kulit kerbau. Design penelitian menggunakan Rancangan Acak Lengkap, dengan perlakuan variasi konsentrasi asam klorida 0.3M, 0.6M, 0.9 M, 1. ABSTRACTThe objective of the research was to identify the effect of the concentration of hydrochloric acid (HCl) on yield and the characteristics of gelatin from buffalo hide. A completely randomized design was used with various concentrations of HCl treatment 0.3M, 0.6M, 0.9 M, 1.2 M and 1.5M, and four times replication, followed by least significant difference test. The HCl concentration gave significantly effect (P<0.05) on yield, viscosity, pH, gel strength and redness of gelatin. The highest yield (29.17%) was obtained at a concentration of HCl 0.9M. The highest gel strength and viscosity at concentration of 1.2M, were 293.41g bloom and 22.17 cp, respectively. The pH of the gelatin were 5.08 to 5.52. The lightness level (L*) was 69.92-70.97, whereas the redness r a n g e d (a*) from 0.55 to 1.54 and the yellowness r a n g e d (b*) from 17.54 to 19.59. The moisture, protein and ash concentrations of gelatin were 7.05±0.07-8.92±0.06%, protein content of 83.38±0.06 -91.11±0.03%, and the ash content 0.53 ± 0.04 to 1.23 ± 0.09%, respectively. Based on the spectra absorption of infrared were detected four areas amides: amide A (3402.43-3448.72 cm-1), amide I (1635.65 cm-1), amide II (1527.62 cm-1) and amide III (1242.16 cm-1). The differences in the concentration of HCl had impact on yield, characteristics and secondary structure of buffalo hide gelatin. The HCl concentration at 0.9M was the optimum condition to obtain the highest yield. The characteristics of buffalo hide gelatin after pretreatment using hydrochloric acid had met the standards of GMIA (2012) .

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Section: Chemical Composition Of Hide and Gelatinmentioning

confidence: 97%

Section: Chemical Composition Of Hide and Gelatinmentioning

confidence: 99%

Section: Chemical Composition Of Hide and Gelatinmentioning

confidence: 99%

See 1 more Smart Citation

The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Mulyani

Setyabudi

Pranoto

et al. 2017

J. Indonesian Trop. Anim. Agric.

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“…The gelatin extract was then concentrated and dried at 60°C for 24-36 h until the formation of a solid sheet. The gelatin sheet was wrapped in plastic and vacuum stored in a desiccator [11].…”

Section: Gelatin Extractionmentioning

confidence: 99%

Optimized High-Performance Liquid Chromatography-Fluorescence Detection Method for the Measurement of Glycine, Proline, and Hydroxyproline Concentrations in Porcine Gelatin

2018

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Objective: The aim of this study is to develop an optimized method for glycine, proline, and hydroxyproline content quantitation in porcine skin gelatin. Methods:Gelatin was isolated from porcine skin by hydrolysis for 24 h in 0.5 M acetic acid, heating in distilled water at 55°C for 3 h, and drying at 60°C. The extract was evaluated by organoleptic tests, Fourier-transform infrared spectroscopy, moisture assay, ash assay, and viscosity test. Gelatin amino acids were derivatized using 9-fluorenylmethylchloroformate-chloride and measured by high-performance liquid chromatography (HPLC) with fluorescence detection using a C18 column after the optimization of the mobile phase composition, flow rate, and detection wavelengths. Results:The optimized parameters for the quantitation of glycine, proline, and hydroxyproline by HPLC with fluorescence detection were as follows: Excitation wavelength, 265 nm; emission wavelength, 320 nm; mobile phase composition acetic buffer: acetonitrile, 55:45; and flow rate, 0.8 mL/min. The average proportional amino acid contents were 28.57±0.74%, 19.24±0.48%, and 2.89±0.33% for glycine, proline, and hydroxyproline, respectively. Conclusion:This method allows for sensitive and accurate quantitation of glycine, proline, and hydroxyproline in porcine skin gelatin samples for quality control and source determination.

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“…Acetic acid concentration of 0.2 M influenced the characteristic of the unicorn leatherjacket skin gelatin [7]. Research by treatment with acetic acid for extraction have been conducted regarding pig skin gelatin [8], goat skin gelatin [9] , fish skin gelatin [10,.11,.12] and chicken legs skin gelatin [13]. This Study aims to weight molecular, amino acids and functional groups profile of gelatin from bovine split hide using variation of acetic acid concentration as curing solution.…”

Section: Introductionmentioning

confidence: 99%

Molecular Weight Distribution, Amino Acid and Functional Groups Profile of Gelatin Bovine Split Hide as Influenced by Acid Pre-Treatment

Wulandari¹,

Erwanto²,

Pranoto³

et al. 2016

Proceeding of the First International Conference on Technology, Innovation and Society

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Gelatin is natural substance obtained from the partial hydrolysis of collagen from the skin, bones and connective tissue. This Study aims to weight molecular, amino acids and functional groups profile of gelatin from bovine split hide using a variation of acetic acid concentration as a curing solution. The materials were gelatin from split bovine hide or hide split of flesh part from tanning industry. The materials were divided into three treatments T1, T2 and T3 (curing in 0.1M; 0.3M; 0.5M acetate acid) for 24 hours, then being extracted graded at 60oC, 70oC, 80oC each for 5 hours. Electrophoresis result showed the distribution molecular weight between 25-40 kDa. Amino acid profile of gelatin was similar to the amino acid composition of collagen with a high level of hydroxyproline. The intensity of the absorption of infrared (FTIR) consisted of functional groups O-H, C=O, C=C, C-H, and C-O. The result of amino acid and functional groups profile gelatin from bovine split hide by curing acetate acid concentration of 0.5 M provided the best characteristics of gelatin.

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The Effects of Acetic Acid Concentration and Extraction Temperature on Physical and Chemical Properties of Pigskin Gelatin

Cited by 44 publications

References 20 publications

The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Optimized High-Performance Liquid Chromatography-Fluorescence Detection Method for the Measurement of Glycine, Proline, and Hydroxyproline Concentrations in Porcine Gelatin

Molecular Weight Distribution, Amino Acid and Functional Groups Profile of Gelatin Bovine Split Hide as Influenced by Acid Pre-Treatment

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