The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

Abstract

ABSTRAKTujuan penelitian adalah untuk mengidentifikasi variasi konsentrasi asam klorida terhadap yield dan sifat fisikokimia gelatin kulit kerbau. Design penelitian menggunakan Rancangan Acak Lengkap, dengan perlakuan variasi konsentrasi asam klorida 0.3M, 0.6M, 0.9 M, 1. ABSTRACTThe objective of the research was to identify the effect of the concentration of hydrochloric acid (HCl) on yield and the characteristics of gelatin from buffalo hide. A completely randomized design was used with various concentrations of HCl treatment 0.3M, 0.6M, 0.9 M, 1.2 M and 1.5M, and four times replication, followed by least significant difference test. The HCl concentration gave significantly effect (P<0.05) on yield, viscosity, pH, gel strength and redness of gelatin. The highest yield (29.17%) was obtained at a concentration of HCl 0.9M. The highest gel strength and viscosity at concentration of 1.2M, were 293.41g bloom and 22.17 cp, respectively. The pH of the gelatin were 5.08 to 5.52. The lightness level (L*) was 69.92-70.97, whereas the redness r a n g e d (a*) from 0.55 to 1.54 and the yellowness r a n g e d (b*) from 17.54 to 19.59. The moisture, protein and ash concentrations of gelatin were 7.05±0.07-8.92±0.06%, protein content of 83.38±0.06 -91.11±0.03%, and the ash content 0.53 ± 0.04 to 1.23 ± 0.09%, respectively. Based on the spectra absorption of infrared were detected four areas amides: amide A (3402.43-3448.72 cm-1), amide I (1635.65 cm-1), amide II (1527.62 cm-1) and amide III (1242.16 cm-1). The differences in the concentration of HCl had impact on yield, characteristics and secondary structure of buffalo hide gelatin. The HCl concentration at 0.9M was the optimum condition to obtain the highest yield. The characteristics of buffalo hide gelatin after pretreatment using hydrochloric acid had met the standards of GMIA (2012) .

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

“…The pretreated hides were soaked in distilled water (1:4 w/v) at 65°C (Memmert WNB7-45 type water bath) for 5 h, followed by further soaking at 70°C for 5 h in preparation for the second extraction step. The extracted gelatin was sieved (< 200 mesh) and dried in a cabinet drier at 50-55°C for 48 h until the moisture less than 12% ( Mulyani et al , 2017 ), and the yield was calculated using the following formula ( Kim et al , 2012 ; Ktari et al , 2014 ):…”

Ekstraksi kolagen dari kulit kerbau menggunakan asam asetat

“…Metode yang digunakan mengacu pada penelitian Mulyani et al (2017a), yaitu kulit kerbau dicuci menggunakan air mengalir untuk menghilangkan larutan garam yang merupakan pengawet pada kulit. Kemudian kulit direndam dalam air kapur 2% (b/v) untuk menghilangkan rambut pada kulit.…”

“…Identifikasi karakteristik kolagen dari kulit kerbau meliputi nilai rendemen yang sesuai dengan penelitian Mulyani et al (2017b) dan kadar air yang sesuai panduan AOAC (2005). Uji berat molekul menggunakan SDS-PAGE dengan metode Laemmli (1970) dan uji gugus fungsional dengan menggunakan FTIR yang mengacu pada penelitian Mulyani et al (2017a).…”

“…sekunder (Mulyani et al, 2017a). Pada pengujian gugus fungsional kolagen yang dilakukan pada salah satu perlakuan, yaitu ekstraksi dengan asam asetat pada konsentrasi 1,5 M selama 72 jam, diperoleh grafik sebagaimana disajikan pada Gambar 2.…”

Comparative Quality of Proteins and Morphological Structures of Gelatin From Sheepskin With Acid and Alkaline Treatment

“…The average level of gelatin protein in sheepskin type A in this study is higher when compared with the results of research Zilhadia et al, (2018) which produces an average level of goatskin protein that is 86.58%, this is caused by differences in raw materials, methods pretreatment and duration of soaking in HCl solution. Long immersion time and pretreatment material concentration cause collagen fibres to shrink and break into irregular structures so that they undergo a perfect dissolution process during the process of hydrolysis of skin collagen (Mulyani et al, 2017).…”