The effects of acarbose and Rumex patientia L. on ultrastructural and biochemical changes of pancreatic B cells in streptozotocin-induced diabetic rats

Arulselvan

JOURNAL OF HEALTH SCIENCE

et al. 2006

172

The present study was aimed to evaluate the anti-diabetic potential of Terminalia chebula (T. chebula) fruits on streptozotocin (STZ)-induced experimental diabetes in rats. Oral administration of ethanolic extract of the fruits (200 mg/kg body weight/rat/day) for 30 days significantly reduced the levels of blood glucose and glycosylated hemoglobin in diabetic rats. Determination of plasma insulin levels revealed the insulin stimulating action of the fruit extract. Also, the alterations observed in the activities of carbohydrate and glycogen metabolising enzymes were reverted back to near normal after 30 days of treatment with the extract. Electron microscopic studies showed significant morphological changes in the mitochondria and endoplasmic reticulum of pancreatic β cells of STZinduced diabetic rats. Also, a decrease in the number of secretory granules of β-cells was observed in the STZinduced diabetic rats and a these pathological abnormalities were normalized after treatment with T. chebula extract. The efficacy of the fruit extract was comparable with glibenclamide, a well known hypoglycemic drug.

Section: Discussionsupporting

confidence: 92%

Anti-Diabetic Activity of Fruits of Terminalia chebula on Streptozotocin Induced Diabetic Rats

Arulselvan

JOURNAL OF HEALTH SCIENCE

et al. 2006

172

Braz. arch. biol. technol.

“…There are also studies about diabetes causes histological alterations in different tissues (Degirmenci et al 2005;Arnal et al 2010;Shanmugam et al 2011). In STZ-treated control rats the serum urea, uric acid, creatinine levels and kidney MDA contents, histopathological results significantly increased; kidney antioxidant enzyme activities significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

“…In the STZ-induced diabetes, MDA levels increase Apaydın et al 2016 ). Also, antioxidant defence system is significantly altered (Degirmenci et al 2005). In the long term, diabetes leads to complications in lots of organ systems.…”

Section: Introductionmentioning

confidence: 99%

Lycopene Protects the Diabetic Rat Kidney Against Oxidative Stress-mediated Oxidative Damage Induced by Furan

Pandır

Ünal

Baş

2016

Furan is a food and environmental contaminant and a potent carcinogen in animals. Lycopene is one dietary carotenoid found in fruits such as tomato, watermelon and grapefruit. The present study was designed to explore the protective effect of lycopene against furan-induced oxidative damage in streptozotocin (STZ)-induced diabetic rat kidney. At the end of the experimental period (28 days), we found that lycopene markedly decreased the malondialdehide (MDA) levels in the kidney, urea, uric acid and creatinine levels in the serum of furan-treated rats. The increase of histopathology in the kidney of furan-treated rats were effectively suppressed by lycopene. Furthermore, lycopene markedly restored superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities in the kidney of furan-treated rats.In conclusion, these results suggested that lycopene could protect the rat kidney against furan-induced injury by improving renal function, attenuating histopathologic changes, reducing MDA production and renewing the activities of antioxidant enzymes.

Journal of Applied Sciences and Environmental Management

“…Our histology results shows enormous liver tissue damage in the Paracetamol treated group which was also found to be reduced in groups that were administered with stem bark extracts of Ficus exasperata. (Degirmenchi et al 2002). The increase in aminotransferases levels may be due to the cellular damage in the liver caused by Paracetamol induced hepatotoxicity.…”

Section: Sacrifice Of Animalsmentioning

confidence: 99%

Stem Bark Extracts of <i>Ficus exasperata</i> protects the Liver against Paracetamol induced toxicity in Wistar Rats

Enogieru¹,

Charles²,

Omoruyi³

et al. 2015

ABSTRACT:Ficus exasperata is an important medicinal plant with a wide geographical distribution in Africa particularly in Nigeria. In this study, aqueous stem bark extracts of Ficus exasperata were administered to investigate its hepatoprotective effects on Paracetamol induced liver toxicity in Wistar rats. A total of Twenty Five Wistar rats were randomly divided into five groups labeled A-E and kept in a well ventilated room. Group A served as control and were treated with distilled water. Rats in groups B-E were all treated with Paracetamol (800mg/kg body weight) but rats in group C, D and E were however pretreated with Silymarin (50 mg/kg bw), 100mg/kg bw aqueous stem bark extracts of Ficus exasperata and 200mg/kg bw aqueous stem bark extracts of Ficus exasperata respectively one hour before Paracetamol administration for fourteen days. Animals were sacrificed twenty four hours after the last treatment. Blood samples were collected into heparinized bottles for biochemical estimation of liver enzymes and the liver was harvested for routine histological examination. Paracetamol produced significant changes in biochemical parameters (increases in serum Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP), with a reduction in Total protein) and Liver histology (damage to hepatocytes). Pretreatment with Silymarin and aqueous stem bark extracts of Ficus exasperata significantly prevented the biochemical and histological changes induced by Paracetamol in the liver. In conclusion, our histological and biochemical findings indicate that aqueous stem bark extracts of Ficus exasperata possesses hepatoprotective properties. © JASEM http://dx.doi.org/10.4314/jasem.v19i1.20