The effects of a wheat germ rich diet on oxidative mtDNA damage, mtDNA copy number and antioxidant enzyme activities in aging<i>Drosophila</i>

Mutlu, Ayşe Gül

doi:10.1556/abiol.64.2013.1.1

Cited by 4 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is no human study assessing the effects of WG on blood pressure in the literature. Although WG has strong antioxidant properties (Cheng et al, ; Mutlu, ), our findings did not show any significant reduction in MDA following WG consumption. However, within‐group comparison showed a significant rise in TAC levels post‐WG consumption.…”

Section: Discussioncontrasting

confidence: 82%

The effects of wheat germ supplementation on metabolic profile in patients with type 2 diabetes mellitus: A randomized, double‐blind, placebo‐controlled trial

Mohammadi

Karimifar

Heidari³

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

The aim of the present trial was to examine the effects of wheat germ (WG) consumption on metabolic control and oxidative stress status of type 2 diabetes mellitus (T2DM) patients. Eighty participants with T2DM were randomly allocated to receive 20‐g WG (n = 40) or placebo (n = 40) in a randomized double‐blind clinical trial for 12 weeks. Serum lipid profiles, glycaemic indices, total antioxidant capacity, and malondialdhyde (MDA) were assessed. A total of 75 subjects completed the trial. Compared with the placebo, WG consumption led to significant reduction in total cholesterol (TC) concentrations (p = .04). There was a trend regarding TC to high density lipoprotein ratio (p = .08) following 12 weeks WG consumption, although they were not statistically significant after correcting for multiple testing. In addition, within‐group comparison revealed a significant rise in total antioxidant capacity concentration (p = .001) in WG group. We observed no significant effects of WG intake on glycaemic status, blood pressure, MDA, triglyceride, and low density lipoprotein levels. WG consumption for 12 weeks could decrease serum TC levels and had no significant effects on other metabolic variables and MDA in patients with T2DM. Though observed health benefit effects were small, it might lead to a major impact on wider public health.

show abstract

Section: Discussioncontrasting

confidence: 82%

The effects of wheat germ supplementation on metabolic profile in patients with type 2 diabetes mellitus: A randomized, double‐blind, placebo‐controlled trial

Mohammadi

Karimifar

Heidari³

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…Primers for Drosophila mtDNA small fragment (100 bp) were (Mutlu, 2012a;Mutlu, 2012b;Mutlu, 2013); 11426 5'-TAAGAAAATTCCGAGGGATTCA -3' 11525 5'-GGTCGAGCTCCAATTCAAGTTA -3'…”

Section: Methodsmentioning

confidence: 99%

“…Primers for Drosophila mtDNA small fragment (100 bp) were (Mutlu, 2012a;Mutlu, 2012b;Mutlu, 2013); 11426 5'-TAAGAAAATTCCGAGGGATTCA -3' 11525 5'-GGTCGAGCTCCAATTCAAGTTA -3' Primers for large fragment (10629 bp) were (Mutlu, 2012a;Mutlu, 2012b;Mutlu, 2013); 1880 5'-ATGGTGGAGCTTCAGTTGATTT -3' 12508 5'-CAACCTTTTTGTGATGCGATTA -3' For long fragment PCR amplification, DNA was denatured initially at 98°C for 1 minute; the material then underwent 21 PCR cycles of 98°C for 10 seconds, 52°C for 45 seconds, and 68°C for 5 minutes. Final extension was allowed to proceed at 68°C for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

Civa Klorürün mtDNA'da Akut Etkisi

Mutlu¹,

Yildiz²

2017

Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi

View full text Add to dashboard Cite

Mercury is a highly toxic environmental pollutant. Molecular mechanisms of mercury toxicity are assorted. Basically, they block essential functional groups in biomolecules and also displace essential metal ions from them. Mercuric ion is known as one of the strongest thiol-binding agents. Although the oxidative properties of mercury have been studied and accepted the actual process of ROS generation is still unclear. The aim of the current study is to examine the acute effects of mercury chloride exposure on mtDNA damage and mtDNA copy number in Drosophila. Quantitative PCR method was used to measure mtDNA damage. In the mercury chloride application groups, mtDNA damage and mtDNA copy number were slightly greater than the control group but the difference was not statistically significant. We demonstrated that the mercury chloride application does not generate the damage on mtDNA of Drosophila melanogaster in 24 hours treatment.Keywords: Mercury, mtDNA damage, mtDNA copy number, Drosophila Civa Klorürün mtDNA'da Akut Etkisi ÖZ Civa çok zehirli bir çevresel kirleticidir. Civa toksisitesinin moleküler mekanizmaları çeşitlidir. Esasen, biyomolekül-lerdeki temel fonksiyonel grupları bloke eder ve temel metal iyonlarını onlardan uzaklaştırır. Civa iyonu, en güçlü tiol bağlayıcı ajanlardan biri olarak bilinir. Civanın oksidatif özellikleri araştırılmış ve kabul edilmiş olsa da, ROS oluşu-munun gerçek süreci hala belirsizdir. Bu çalışmanın amacı, civa klorür maruziyetinin, Drosophila'daki mtDNA hasarı ve mtDNA kopya sayısı üzerindeki akut etkilerini incelemektir. Çalışmamızda mtDNA hasarını ölçmek için kantitatif PCR yöntemi kullanıldı. Sonuçlara göre, civa klorür uygulama gruplarında mtDNA hasarı ve mtDNA kopya sayısı kontrol grubundan biraz daha fazlaydı, ancak bu fark istatistiksel olarak anlamlı değildi. Buna dayanarak, civa klorür uygulamasının Drosophila melanogaster'in mtDNA'sında, akut hasar oluşturmadığı sonucuna varılmıştır.

show abstract

Effects of glutathione on mitochondrial DNA and antioxidant enzyme activities in Drosophila melanogaster

Yildiz

2022

International Journal of Secondary Metabolite

View full text Add to dashboard Cite

The free radical theory in aging assumes that the accumulation of macromolecular damage induced by toxic reactive oxygen species plays a central role in the aging process. The intake of nutritional antioxidants can prevent this damage by neutralizing reactive oxygen derivatives. Glutathione (GSH; en-L-Glutamyl-L-cysteinyl glycine) is the lowest molecular weight thiol in the cells and as a cofactor of many enzymes and a potent antioxidant plays an important role in maintaining normal cell functions by destroying toxic oxygen radicals. In this study, the effects of GSH on SOD, GST and catalase enzymes and mtDNA damage were investigated at various time intervals by giving reduced glutathione to Drosophila. It was observed that 3-week GSH administration did not have a statistically significant effect on SOD and GST activities whereas GSH application decreased the catalase enzyme activities significantly. Although the decrease in antioxidant capacity with age was observed in SOD and catalase enzymes, such a situation was not observed in GST enzyme activities. There was no statistically significant difference between the control and GSH groups in mtDNA copy number values, while in the GSH group, oxidative mtDNA damage was high. These results may be due to the prooxidant effect of GSH at the dose used in this study.

show abstract

The effects of a wheat germ rich diet on oxidative mtDNA damage, mtDNA copy number and antioxidant enzyme activities in agingDrosophila

Cited by 4 publications

References 25 publications

The effects of wheat germ supplementation on metabolic profile in patients with type 2 diabetes mellitus: A randomized, double‐blind, placebo‐controlled trial

The effects of wheat germ supplementation on metabolic profile in patients with type 2 diabetes mellitus: A randomized, double‐blind, placebo‐controlled trial

Civa Klorürün mtDNA'da Akut Etkisi

Effects of glutathione on mitochondrial DNA and antioxidant enzyme activities in Drosophila melanogaster

Contact Info

Product

Resources

About