The Effects of a Promoter of Cell Differentiation and Selected Hormones on Human Cytomegalovirus Infection Using an In Vitro Cell System

Forbes, Betty A.; Bonville, Cynthia A.; Dock, Nancy L.

doi:10.1093/infdis/162.1.39

Cited by 36 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been suggested that productive CMV infection is suppressed in early gestation, and the suppressive effect wanes with advancing gestation [Stagno et al, 1975;Stagno, 1995]. In addition, transient depression of maternal humoral or cell-mediated immune responses to CMV during late pregnancy [Faix et al, 1983;Kumar et al, 1984] and hormonal changes during gestation [Tanaka et al, 1984;Mackowiak et al, 1987;Forbes et al, 1990] are suggested to enhance the reactivation of latent CMV Fig. 1.…”

Section: Discussionmentioning

confidence: 99%

Detection of cytomegalovirus DNA in human placenta

Kumazaki

Ozono

Yahara

et al. 2002

Journal of Medical Virology

View full text Add to dashboard Cite

Although human cytomegalovirus (CMV) is one of the most common causes of viral intrauterine and perinatal infection, its distribution in the placenta is poorly understood. The purpose of this study was to determine the frequency of CMV DNA positivity in placentas, to demonstrate the localization of the viral genome, and to identify the clinical features related to placental CMV. A total of 254 placentas from 231 mothers were investigated, and the maternal serum CMV immunoglobulin antibodies were measured. Specimens from both the placental parenchyma and the placental membrane close to the ruptured site in each placenta were examined for the presence of CMV DNA using dot blot hybridization after PCR amplification. None of 57 placentas from seronegative mothers was positive for CMV DNA. Of 197 placentas from seropositive mothers, 60 (30.5%) had CMV DNA in either the parenchyma or the membrane by dot blot analysis. In situ hybridization was carried out on these 60 placentas, and the localization of the viral genome was established in 19; CMV DNA was localized mostly to the villi, including the mesenchyme and trophoblasts, extravillous trophoblasts, and decidual cells. The mean gestational age at delivery was significantly later in the CMV DNA-positive placentas than in the negative placentas (36.9 +/- 5.1 vs 34.7 +/- 6.2 weeks, P = 0.0059). CMV DNA was detected in only 6 of 33 placentas delivered in the second trimester, and all six were associated with either severe maternal nephritis or severe chorioamnionitis. These results suggest that the CMV genome is common in placentas at later gestational ages and in those of earlier gestational ages with certain maternal complications.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of cytomegalovirus DNA in human placenta

Kumazaki

Ozono

Yahara

et al. 2002

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…55 Similarly, cyclosporine use has been associated with CMV disease in some studies, but there are no data available to disentangle the medication from the patients with severe disease in whom it is used. Infliximab has not been associated with an increased risk of CMV reactivation in patients with IBD.…”

Section: How Frequently Is CMV Detected In the Colon Of Patients Withmentioning

confidence: 99%

Cytomegalovirus in inflammatory bowel disease: Pathogen or innocent bystander?

Lawlor

Moss

2010

Inflammatory Bowel Diseases

223

190

View full text Add to dashboard Cite

The role of cytomegalovirus (CMV) in exacerbations of inflammatory bowel disease (IBD) remains a topic of ongoing debate. Current data are conflicting as to whether CMV worsens inflammation in those with severe colitis, or is merely a surrogate marker for severe disease. The interpretation of existing results is limited by mostly small, retrospective studies, with varying definitions of disease severity and CMV disease. CMV colitis is rare in patients with Crohn's disease or mild-moderate ulcerative colitis. In patients with severe and/or steroid-refractory ulcerative colitis, local reactivation of CMV can be detected in actively inflamed colonic tissue in about 30% of cases. Where comparisons between CMV+ and CMV- steroid-refractory patients can be made, most, but not all, studies show no difference in outcomes according to CMV status. Treatment with antiviral therapy has allowed some patients with severe colitis to avoid colectomy despite poor response to conventional IBD therapies. This article reviews the immunobiology of CMV disease, the evidence for CMV's role in disease severity, and discusses the outcomes with antiviral therapy.

show abstract

“…Many investigators have reported that cellular differentiation by the addition of chemicals or cytokines such as phorbol esters, DMSO, retinoic acid, hydrocortisone, concanavalin A, dimethylacetimide, sodium valproate, β-gonadotropin, nerve growth factor or dbcAMP is necessary to induce permissive CMV infection or to increase CMV gene expression and virus replication in primary cells and cell lines (Dutko & Oldstone, 1981 ;Tanaka et al, 1985Tanaka et al, , 1991La Femina & Hayward, 1986 ;Weinshenker et al, 1988 ;Forbes et al, 1990 ;Ibanez et al, 1991 ;Turtinen & Seufzer, 1994 ;Kuntz-Simon & Obert, 1995). Here, we report that pretreatment of the oligodendrocyte cell line HOG with either PMA or dbcAMP\IBMX permitted infection with CMV as judged by the expression of the early and late CMV genes and release of extracellular infectious CMV particles.…”

Section: Discussionmentioning

confidence: 99%

Development of a model for cytomegalovirus infection of oligodendrocytes.

Spiller

Borysiewicz

Morgan

1997

Journal of General Virology

View full text Add to dashboard Cite

The well-characterized human oligodendroglioma (HOG) cell line, cells of which resemble immature oligodendrocytes, was used to investigate the level of permissiveness to human cytomegalovirus (CMV) infection. Expression of CMV genes was incomplete following exposure of HOG cells to CMV, in contrast with results observed with the astroglioma cell line U373-MG (used as a positive control). However, treatment with phorbol 12-myristate 13-acetate (PMA) or with dibutryl cAMP (dbcAMP) plus the

show abstract

The Effects of a Promoter of Cell Differentiation and Selected Hormones on Human Cytomegalovirus Infection Using an In Vitro Cell System

Cited by 36 publications

References 26 publications

Detection of cytomegalovirus DNA in human placenta

Detection of cytomegalovirus DNA in human placenta

Cytomegalovirus in inflammatory bowel disease: Pathogen or innocent bystander?

Development of a model for cytomegalovirus infection of oligodendrocytes.

Contact Info

Product

Resources

About