Detection of cytomegalovirus DNA in human placenta

Kumazaki, Kaori; Ozono, Keiichi; Yahara, Takeshi; Wada, Yoshinao; Suehara, Noriyuki; Takeuchi, Makoto; Nakayama, Masahiro

doi:10.1002/jmv.10212

Cited by 30 publications

(17 citation statements)

References 23 publications

(17 reference statements)

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“…Other studies on cases with previous immunity against the virus showed nearly similar results [21]. Kumazaki et al [10] reported that the CMV genome was detected by PCR analysis in placentas, not only on the maternal side, but also in the villi of seropositive women.…”

Section: Discussionsupporting

confidence: 50%

Detection of HCMV DNA in placenta, amniotic fluid and fetuses of seropositive women by nested PCR

et al. 2006

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Human cytomegalovirus (HCMV) is the most common viral cause of intrauterine infection throughout the world. Its distribution patterns in different clinical samples are poorly understood. This study was performed to determine the frequency of CMV DNA positivity in maternal/fetus sera, placentas and amniotic fluid, together with maternal/fetus serology. Clinical specimens were obtained from 92 pregnant women who delivered by cesarean section. 98% of women and their neonates were HCMV IgG positive and 5.4% of these mothers were IgM positive, while no IgM was detected in neonates of IgM positive mothers. Among the IgG positive mothers, IgM was detected in 3.3% of their fetuses. 5.4% and 3.3% of maternal and fetal sera were HCMV DNA positive, respectively. The three neonates who were positive for HCMV DNA in sera were also positive for HCMV IgM and the PCR of their amnions was positive (p < 0.0001). 9.8% of placenta samples and 4.3% of amniotic fluid specimens were positive for HCMV DNA while among these placenta samples, two amnions were PCR positive (p = 0.046). Our results showed that there is not always a correlation between placenta and amnion infections. This may be due to reactivation of HCMV leading to placenta infection, as all affected placentas do not pass infection to fetuses and amniotic fluids. Detection of HCMV DNA in amnion and fetus plasma and the existence of fetus IgM against HCMV can also occur without clinical symptoms.

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Section: Discussionsupporting

confidence: 50%

Detection of HCMV DNA in placenta, amniotic fluid and fetuses of seropositive women by nested PCR

et al. 2006

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“…In the present study, 69% of first-and secondtrimester placental biopsy samples from uncomplicated pregnancies tested positive for CMV DNA. Others have reported CMV in cervical and placental samples in late gestation, with higher detection rates in abnormal pregnancies [1,39]. Differences in detection rates may be due to the sensitivity of the methods used.…”

Section: Discussionmentioning

confidence: 88%

Viral and Bacterial Pathogens at the Maternal‐Fetal Interface

et al. 2004

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We studied the incidence of pathogenic bacteria and concurrent infections with human cytomegalovirus (CMV) and herpes simplex virus (HSV) type 1 and 2 in biopsy samples from the placenta and decidua of women with healthy pregnancies. By polymerase chain reaction analysis, we found that 38% of placental samples were positive for selected bacteria and viruses. CMV, HSV-1, and HSV-2 were detected in isolation or with bacteria in first- and second-trimester samples. Certain bacteria were detected more often during the second trimester than during the first--Ureaplasma urealyticum, Mycoplasma hominis, and Gardnerella/Bifidobacterium species. In paired samples from first-trimester tissues, the detection rate for viruses, compared with most bacteria, was higher in the decidua than in the adjacent placenta. In contrast, bacteria were more frequently detected in placenta. Analyses of immunoglobulin G isolated from the placenta support the hypothesis that immune responses suppress CMV reactivation in the presence of pathogenic bacteria at the maternal-fetal interface.

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“…Of those TH1 cytokine IL-2 plays a key role in regulating the balance between immunity and tolerance. 35,43,44 In this study, preeclamptic women seropositive for CMV-IgG, the IL-2 concentration was elevated but statistically not significant in comparison with seronegative CMV-IgG level (P > 0.05). Results obtained in this study indicated that the mean concentration of IL-2 was significantly higher in normotensive women seropositive for CMV-IgG as compared to seronegative group (P < 0.05).…”

Section: Discussionmentioning

confidence: 51%

The possible role of cytomegalovirus infection and pro-inflammatory IL_2 cytokine in preeclampsia

Barzangy

Salh

Hamasaeed

et al. 2018

ZJMS

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IntroductionThe origin of preeclampsia, a hypertensive disorder unique to pregnancy is still a matter of debate and numerous theories have suggested. The pathophysiology of the disease involves impaired trophoblast invasive, abnormal genetic polymorphism, vascular endothelial cell activation, immune tolerance by the maternal immune system and an exaggeration of a systematic inflammatory process. Background and Objective: Fragmentary evidence suggests that trophoblast viral infection may play a role in placental dysfunction leading to complications like Preeclampsia. Among these, placental exposure to CMV induces an inflammatory response that precedes invasive trophoblast cell death. This study aimed to assess the frequency of anti-CMV IgG seropositivity and IL2 level in serum in patients with preeclampsia compared to normotensive control pregnant women. Methods: A total of 90 women were enrolled, of which 60 had preeclampsia and 30 normotensive pregnant women as the control. A serum sample was collected from each subject and was investigated for anti-CMV IgG serostatus and IL-2 concentration using Enzyme-Linked Immunosorbent Assay (ELISA) based kits. Results: In preeclamptic and normotensive pregnant women, 33.3% and 16.6% of the tested sera were seropositive for anti CMV IgG antibodies respectively (P = 0.136). Early onset preeclamptic women revealed a high frequency of anti-CMV seropositivity (80%) when compared with late onset Preeclampsia (20%). Cytokine assessment revealed a higher IL-2 level in preeclamptic women seropositive for anti-CMV IgG although statistically was not significant (P = 0.14), but in normotensive women, the IL-2 level was significantly higher in sera seropositive for anti-CMV IgG (P = 0.02). Conclusion: This study delineates a high frequency of anti-CMV IgG antibodies particularly those with early onset preeclampsia; with no significant difference for the IL-2 level.

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Detection of cytomegalovirus DNA in human placenta

Cited by 30 publications

References 23 publications

Detection of HCMV DNA in placenta, amniotic fluid and fetuses of seropositive women by nested PCR

Detection of HCMV DNA in placenta, amniotic fluid and fetuses of seropositive women by nested PCR

Viral and Bacterial Pathogens at the Maternal‐Fetal Interface

The possible role of cytomegalovirus infection and pro-inflammatory IL_2 cytokine in preeclampsia

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