The Effects of 17β-Estradiol and Medroxyprogesterone Acetate Alone and in Combination with α-Chlorohydrin on Reproductive Function in the Male Rat

Hunt, Dorothy M.; Lubicz-Nawrocki, C. M.; Chang, M. C.

doi:10.1095/biolreprod14.5.544

Cited by 11 publications

(8 citation statements)

References 25 publications

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“…Short treatment with estrogen fails to exert any sterility effect [5, 61 in rats and hamsters; however, a prolonged treatment of rats with high doses of estrogen-17P (E,-17/3) did show azoospermia [5]. The present study describes a dose-and duration-dependent response to E2-17p treatment in the rat.…”

Section: Introductionmentioning

confidence: 60%

Effects of Estradiol-17/β on Reproduction in Adult Male Rats

Hunt¹,

Saksena²,

Chang³

1979

Archives of Andrology

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Estradiol-17p (E2-17p) treatment induced dose-related reduction of sperm population and eventual azoospermia, atrophy of accessory organs, and suppressed serum LH and testosterone levels. In spite of all these alterations mating behavior was not affected, although the number of implantation sites paralleled the reduction of sperm population, suggesting that the primary detectable response to E2-17P treatment is related to the steroidogenic components of the testis and the hypothalamic pituitary axis. The duration of treatment is more critical for the induction of azoospermia in the rat than the total dose of E,-17/3.

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Section: Introductionmentioning

confidence: 60%

Effects of Estradiol-17/β on Reproduction in Adult Male Rats

Hunt¹,

Saksena²,

Chang³

1979

Archives of Andrology

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“…However, existing reports have demonstrated that the exposure to exogenous synthetic estrogens mimics hypophysectomy, causing suppression in pituitary gonadotropins (FSH and LH) and testosterone levels (8,(29)(30)(31)(32)(33). Also observed is testicular apoptosis, depletion of germ cells in all stages of the spermatogenic cycle, inhibition of androgenesis (in Leydig cells) and altered testicular enzymatic profile, as well as related accessory sex organ function coinciding with reduction in sperm production, semen characteristics and libido in adult animals (6,7,(34)(35)(36)(37). Likewise, neonatal estrogenization has also contributed to the significant decline in pituitary gonadotropins (FSH and LH) and testicular and accessory sex organs function (38,39) during post-natal development; permanently restricted prostatic growth and testosterone sensitivity in adulthood also exerted its effect at neuroendocrine levels (40,41).…”

Section: Discussionmentioning

confidence: 99%

Effect of Compound Cdri 84/35 and Synthetic Estrogen on the Seminiferous Epithelium of Immature Rat

2002

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Compound CDRI 84/35 (a piperazine derivative--a potent antispermatogenic agent) has been shown to cause significant inhibition in testicular spermatogenesis without affecting Leydig cell and accessory sex organ function in adult rats. The present study was conducted to determine its effect on the germ cell population and Leydig cell morphology in immature rats (40-50 gm) administered CDRI 84/35 (100 mg/kg/day p.o.), synthetic estradiol benzoate (EB; 5 microg/rat/day) and vehicle at the age of 21 days. Animals were killed 24 h later following 7 and 14 days' treatments. Bouin's fixed testes were sectioned (at 5 microm) and stained with PAS-hematoxylin. Quantitative determination of Sertoli Cell-Germ Cell ratio was carried out in 150 round seminiferous tubules in each group of 5 rats. Results revealed a significant decrease in number of the spermatocytes (non-pachytene and pachytene) and early (round) spermatids in step 1-8 of spermiogenesis without affecting Leydig cell morphology in rats administered CDRI 84/35 for 7 and 14 days as compared to corresponding controls. In contrast, the testes of rats injected with synthetic EB, caused a marked inhibition in these meiotic and post-meiotic germ cell types, as well as in the diameters of round seminiferous tubules, and Leydig cells nuclei (only in 14 days treatment), and testicular weight on autopsy days 8 and 15 as compared to CDRI 84/35-treated rats. While the number of pre-meiotic spermatogoniae was observed to be slightly decreased after only 14 days treatment in both CDRI 84/35 and EB treatment groups, the Sertoli cell number did not show any significant change as compared to controls. The present investigation confirms the antispermatogenic effect of compound CDRI 84/35 in immature rats similar to that reported in adult rats. Marked inhibition in pachytene spermatocytes and other testicular parameters following synthetic estrogen treatment might be due to its antiandrogenic action, contrasting with the non-hormonal profile of CDRI compound.

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“…To determine sperm population in the excurrent ducts from one side of each male, the ductus deferens were flushed with 1 mlO.995 NaCl solution and the epididymides, separated in the middle of corpus into caput and cauda segments, were macerated in 2 ml 0.9% NaCl solution. After the motility of spermatozoa was noted, the suspension was diluted for counting the number of spermatozoa with a haemocytometer [7].…”

Section: Effects Of Steroids Treatments In Male Rats 313mentioning

confidence: 99%

“…Various progestogens including daily injections of medroxyprogesterone acetate (Provera) for four weeks impaired spermatogenesis and suppressed mating behavior in rats but Provera (1 mghnjectiodanimal) administered every fifth day for 25 days did not affect fertility [7]. Even though Provera inhibited spermatogenesis without disturbing sexual function [ 6 ] , conflicting observations of slight suppression of spermatogenesis also have been reported [ 131.…”

Section: Introductionmentioning

confidence: 99%

“…Even though Provera inhibited spermatogenesis without disturbing sexual function [ 6 ] , conflicting observations of slight suppression of spermatogenesis also have been reported [ 131. Estradiol 17p administered for 25 days impaired spermatogenesis and induced sterility in the male rats [7]. A total disappearance of spermatozoa in the caput epididymis of rats injected daily with ethynodiol diacetate alone or in combination with testosterone propionate for 42 days was encountered [18].…”

Section: Introductionmentioning

confidence: 99%

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Endocrinological and Physiological Features after Steroid Treatment of Male Rats

Hunt¹,

Lau²,

Saksena³

et al. 1978

Archives of Andrology

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The effects of medroxyprogesterone acetate (Provera), testosterone propionate (TP), ethinyl estradiol (EE), and ethynodiol diacetate (ED) treatments on sperm population in different segments of the male rat reproductive tract, reproductive organ weights, circulating androgens and fertility were studied. Ten pg TP given for five days reduced the sperm population and organ weights. A marked reduction in the number of sperm and reproductive organ weights was observed in males orally treated with estrogens. Only long-term (20 days) treatment with Provera ( 1 mglday) significantly reduced sperm population and reproductive organ weights.Combination of TP and Provera resulted in a more pronounced reduction in sperm counts and organ weights. Among steroids studied, estrogen was the only compound which suppressed fertility and circulating steroid levels.

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The Effects of 17β-Estradiol and Medroxyprogesterone Acetate Alone and in Combination with α-Chlorohydrin on Reproductive Function in the Male Rat

Cited by 11 publications

References 25 publications

Effects of Estradiol-17/β on Reproduction in Adult Male Rats

Effects of Estradiol-17/β on Reproduction in Adult Male Rats

Effect of Compound Cdri 84/35 and Synthetic Estrogen on the Seminiferous Epithelium of Immature Rat

Endocrinological and Physiological Features after Steroid Treatment of Male Rats

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