Endocrinological and Physiological Features after Steroid Treatment of Male Rats

Hunt, Dorothy M.; Lau, I.F.; Saksena, S.K.; Chang, M. C.

doi:10.3109/01485017808988351

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…This effect is also explained by the metabolic properties of testosterone and its metabolites. Simultaneous treatment of TP and fruit extract reduced the weight as function is naturally influenced by androgen [31]. TP treatment along with administration of fruit extract reduced the weight of the accessory reproductive organs in castrated rats in comparison to TP-treated castrated rats proves the antiandrogenic nature of the extract.…”

Section: Discussionmentioning

confidence: 95%

Induction of Reversible Antifertility with a Crude Ethanol Extract of <i>Citrullus colocynthis</i> Schrad Fruit in Male Rats

Chaturvedi¹,

Mali

Ansari

2003

Pharmacology

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A crude 50% ethanol extract of Citrullus colocynthis Schrad was administered orally to male albino rats for evaluation of antifertility effects. The animals were divided into five groups: group A was a vehicle-treated control group; treatment groups B, C, and D received 100 mg/kg/day C. colocynthis extract for periods of 20, 40, and 60 days, respectively, and group E animals received the extract at 100 mg/kg/day for 60 days followed by 60 days of recovery. For androgenicity evaluation of the extract, the animals were divided into four groups: group F animals were castrated 30 days before the experiment to serve as controls, and group G, H, and I were subjected to castration 30 days before the experiments, followed by administration of fruit extract (100 mg/kg/day p.o.), testosterone propionate (0.01 mg/rat/alternate day s.c.), and fruit extract along with testosterone propionate, respectively, for 30 days. Significantly reduced cauda epididymis sperm motility and density, number of pups, fertility, and circulatory levels of testosterone were observed in all treatment groups. The weights of testes, epididymis, seminal vesicle, and prostate were significantly decreased in groups B, C, and D. The weights of all organs in the different groups of the androgenicity study were markedly decreased in group F when compared with group A, in group G when compared with group F, and in group I when compared with group H, and increased in group H when compared with group F. The serum testosterone levels also showed a similar pattern. The concentration of testicular cholesterol was significantly elevated, while protein, sialic acid, acid and alkaline phosphatase concentrations were decreased. The histoarchitecture of the testes showed degenerative changes in the seminiferous epithelium, arrest of spermatogenesis at the secondary spermatocyte stage, cytolysis, and the lumen filled with eosinophilic material. Histometric parameters except Sertoli cell nuclear area and number of round spermatids showed marked alterations. All altered parameters restored to normal in group E. No changes were observed in body weight, litter size, hematology, and serum biochemistry. In conclusion, a 50% ethanol extract of C. colocynthis showed an antiandrogenic nature, thereby reduced reversible infertility in male albino rats.

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Section: Discussionmentioning

confidence: 95%

Induction of Reversible Antifertility with a Crude Ethanol Extract of <i>Citrullus colocynthis</i> Schrad Fruit in Male Rats

Chaturvedi¹,

Mali

Ansari

2003

Pharmacology

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“…The decrease in the weight of seminal vesicles, both full and void of its secretory contents, show that flutamide led to depression of cell division and regeneration of the organ and also its secretory activity. The accessory sex organs have been reported to be androgen dependent and thus reflects the availability of androgens (Hunt et al -1978). The weights of the accessory sex organs in castrated rats have been widely used as a bioassay for androgenic and antiandrogenic compounds (Neumann and Steinbeck -1974).…”

Section: Discussionmentioning

confidence: 99%

Involvement of Lysosomal Enzymes in Flutamide-Induced Stimulation of Rat Testis

Mathur

Chattopadhyay

2009

Andrologia

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Zusammenfassung Beteiligung lysosomaler Enzyme bei Flutamid‐induzierter Stimulation des Rattenhodens Das Antiandrogen Flutamid läßt den Plasmaspiegel von LH ansteigen, stimuliert die Steroidbiosynthese im Hoden und hebt die Wirkung von Androgen auf die Zielorgane auf. Flutamid wurde Ratten verabreicht, um die intrazellulären Mechanismen im Hoden zu beobachten, wo die Steroidsyntheserate in Gegenwart des Antiandrogens erhöht war. Das Gewicht der ventralen Prostata sowie der Bläschendrüsen zeigte die antiandrogenen Auswirkungen von Flutamid. Jedoch spiegelte sich das Vorhandensein von Testosteron in der erhöhten Aktivität der β‐Glucuronidase der Niere wieder. Das Gewicht der Hoden und die Inkorporation von 32P in die Nukleinsäurefraktion waren nach 45 Minuten erhöht. Die Konzentration der DNA des Hoden und der Ascorbinsäure hatte abgenommen. Die Aktivität der lysosomalen Fazyme, sauren Phosphatase und β‐Glucuronidase war erhöht. Unter Flutamid kam es demnach zu einem Zustand, in dem ein Wechsel der lysosomalen Stabilität in Verbindung mit einer Veränderung der Hodenfunktion gezeigt wurde.

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“…Flutamide treatment in the adult rat exerted a strong inhibition on the epididymis, seminal vesicles, and ventral prostate as evidenced by the ANDROLOGIA 24, 113-116 (1992) decrease in weights. It has been reported that the accessory sex organs are androgen dependent and thus reflect the availability of androgens (Hunt et al, 1978). The antiandrogenic effects of flutamide on the male sex structures were explained as the consequence of inhibition of androgen uptake and/or inhibition of nuclear binding of the androgens in the target tissues (Peets et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

Effect of flutamide on the physiological status of epididymis and epididymal sperms

2009

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Flutamide, a pure antiandrogen, increases the levels of plasma luteinizing hormone but antagonizes the biological expression of androgen on target organs. Flutamide was administered to rats to study the effect of altered availability of hormones on the functional status of epididymis. The weights of ventral prostate, seminal vesicles and epididymis showed antiandrogenic effects of flutamide. However, increased activity of kidney P-glucuronidase reflected increased availability of testosterone. The concentrations of protein and DNA along with the activities of acid phosphatase and hyaluronidase decreased in flutamide-treated rats. The activities of acid phosphatase and hyaluronidase in epididymal sperms along with protein concentration increased in flutamide-treated rats. Alteration of epididymal function by treatments affecting lyso-soma1 stability was indicated.undertaken to evaluate the effects of altered availability of hormones on the physiology of epididymis and epididymal sperms.

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Endocrinological and Physiological Features after Steroid Treatment of Male Rats

Cited by 7 publications

References 16 publications

Induction of Reversible Antifertility with a Crude Ethanol Extract of <i>Citrullus colocynthis</i> Schrad Fruit in Male Rats

Induction of Reversible Antifertility with a Crude Ethanol Extract of <i>Citrullus colocynthis</i> Schrad Fruit in Male Rats

Involvement of Lysosomal Enzymes in Flutamide-Induced Stimulation of Rat Testis

Effect of flutamide on the physiological status of epididymis and epididymal sperms

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