The Effectiveness of the Growth of a Consortium of Bacillus cereus Bacteria with Different Protein Sources

Abstract

One of the benefits of probiotic bacteria is to become a source of protein that can be used as a product in the field of biotechnology with high use value. Proteins derived from bacteria are less widely used compared to animals and fungi. The aim of this study is to analyze the composition of the media and the optimal time for the growth of a consortium of Bacillus cereus bacteria. The experimental method used is a consortium of B. cereus bacteria isolates (5 Strains) six treatments in each B. cereus consortium, namely the addition of a different protein source (eggs and skim milk, the same carbohydrate source, namely Sago) and 3 different concentrations in each protein source (8%, 10%, and 12%) so that the treatment obtained was 6 treatments with 3 replications in each treatment. Measurement of bacterial culture growth was carried out every 6 hours for 24 hours using two methods, namely the TPC method and bacterial cell biomass. Optimal growth was found in sago media which was added in different concentrations, namely 12% due to growth in this medium which was close to the same results as growth in positive control. Growth on biomass measurements showed similar results to the growth pattern similar to TPC. While in milk sago media, the growth is less because the exponential and stationary phases are shorter.

Utilization of molasses wastewater for medium production of Bacillus cereus

“…The growth medium for B. cereus was made with material combinations containing molasses waste, skim milk, Vitamin B12, K2HPO4, KH2PO4, and NaCl [12]. All materials were sterilized using an autoclave at a temperature of 121°C and a pressure of 1 atm.…”

“…Bacterial growth is the increase in the number of bacterial cells in conditions when proper nutrients and environment are provided. The growth of bacteria is defined as an increase in the number of bacteria in a population because cells are replicated to be many cells [12].…”

“…Bacterial growth is the increase in the number of bacterial cells in conditions when proper nutrients and environment are provided. The growth of bacteria is defined as an increase in the number of bacteria in a population because cells are replicated to be many cells [12]. The two types of bacteria treatment showed that the B. cereus consortium was more effective in using a molasses medium than the B. cereus SN7.…”