The effectiveness of poloxamer 407-based new anti-adhesive material in a laminectomy model in rats

Abstract

Purpose In the laminectomy model in rats, to verify antiadhesive effectiveness of the new material, a mixture composed of poloxamer 407, calcium chloride, and xanthorrhizol, we compared it with that of commercially used solution form anti-adhesive agent GUARDIX-SL, Biorane. Materials and methods A total of 108 Sprague-Dawley rats (SD rats) were divided equally into three groups: negative control group (NCG), positive control group (PCG), and experimental group (EG). After exposing the dura on L4 level, we closed the wound shortly after hemostasis, after administering the anti-adhesive agent. To evaluate effectiveness, 18 SD rats from each group were killed after 1 week of rearing. Nine were examined by grading of gross adhesion and the other nine, by grading of histological adhesion. The degree of adhesion in the remainder of 18 SD rats in each group was examined with the same method after four weeks of rearing.Results Comparing the degree of adhesion after growth for 1 week, the gross and histological adhesion of the EG was lower than that of the NCG. There was no statistical significance in the gross score (P = 0.63), but there was statistical significance in the histological score (P = 0.04). The EG showed similar or improved degrees of adhesion in comparison with the PCG. In comparison after growth for 4 weeks, although gross adhesion of the EG was not significantly lower than the NCG, histological adhesion was remarkably low in the EG (P = 0.01). The EG showed similar or improved degrees of gross and histological adhesion in comparison to that of the PCG. (P = 0.20, 0.07). Conclusion The new anti-adhesive material showed similar or improved effectiveness with the existing agents for commercial use. This result suggests that the new antiadhesive material will be a successful candidate as a future product for clinical use.

Antiadhesive Property of Photoreactive Azidophenyl Low‐Molecular‐Weight Chitosan in Rabbit Laminotomy Model

“…We compared the results of P-ALMC as an anti-adhesive agent with HA-CMC (Guardix, Hanmi Pharmacy, Republic of Korea). It is well known that HA-CMC has been reported to be useful in preventing adhesions in experimental studies [1,6,7,20,22] and is in clinical use in the field of spine surgery [23]. Our newly developed P-ALMC demonstrated similar results to those observed in HA-CMC group in a rabbit model.…”

To what extent topical application of cova™ and tisseel® cause spinal peridural fibrosis? comparison with adcon® gel: an experimental study in rat model.

“…Many factors play a role in amount of peridural scar formation such as postoperative hematoma, postoperative infection and surgical techniques. Improved minimally invasive surgery techniques, many agents and mechanical barriers have been used to accomplish this bothering problem (5,6,12,14,15,17,19,26,(29)(30)(31)33).…”

Prevention of intrauterine post-surgical adhesions in hysteroscopy. A systematic review

“…Carboxycellulose with hyaluronic acid is a well-known anti-adhesive material with long-lasting action for about 7 days that is used to separate abutting tissue surfaces [44]. Moreover, this combination has demonstrated a preventive effect on the formation of adhesion in a various surgical fields [45,46].…”