The Effective Charge Number and Diffusion Coefficient of Cationic Cytochrome c in Aqueous Solution.

Ak, Kontturi; Kontturi, Kyösti; Niinikoski, Pasi; Savonen, A; Vuoristo, Mikko

doi:10.3891/acta.chem.scand.46-0348

Cited by 16 publications

(10 citation statements)

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“…Results of the D p values obtained using both models are collected in Table 5. All coefficients are much lower than that of unsupported cyt c in aqueous solution (2 10 -6 cm 2 s -1 at 30 ºC and 100 mM ionic strength) [45]. The information of the two models is quite different.…”

Section: Immobilisation Of Cytochrome C On the Carbon Xerogelsmentioning

confidence: 77%

Protein adsorption and activity on carbon xerogels with narrow pore size distributions covering a wide mesoporous range

Ramírez-Montoya

Concheso

Alonso-Buenaposada

et al. 2017

Carbon

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Section: Immobilisation Of Cytochrome C On the Carbon Xerogelsmentioning

confidence: 77%

Protein adsorption and activity on carbon xerogels with narrow pore size distributions covering a wide mesoporous range

Ramírez-Montoya

Concheso

Alonso-Buenaposada

et al. 2017

Carbon

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“…The way in which this influences the voltammetric response depends on the stereochemistry of interaction and the conformation necessary for electron transfer to take place. Some investigations on this theme have been undertaken [8][9][10][11][12][13][14]. In particular, it has been stated that conformation and effective charge can be influenced by the temperature [8 -10]; it has also been found that deterioration of cytochrome c is faster the higher the temperature [6].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behaviour of cytochrome c at electrically heated microelectrodes

Voß

Gründler

Brett

et al. 1999

Journal of Pharmaceutical and Biomedical Analysis

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The structural changes in cytochrome c with temperature have been been followed using a recently developed electrically-heated microelectrode sensor. Differential pulse voltammetry was used to perform electrochemical measurements of cytochrome c oxidation at different temperatures at heated bare gold electrodes contained in phosphate-buffered cytochrome c solution at room temperature. The voltammetric response shows the onset of unfolding and a marked dependence of the signal on electrode temperature. This augurs well for applications of heated electrodes as local probes in the study of the temperature dependence of electron transfer processes of other redox proteins, avoiding problems of bulk deterioration.

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“…We performed further clearance studies with a two‐membrane chip, which increases clearance and helps to accurately measure values for molecules with smaller diffusion coefficients like vitamin B12, cytochrome c and insulin. The results are shown in Figure b where it is shown that as the diffusion coefficient decreases, so does the clearance with FITC‐insulin ( D = 8.2 × 10 −7 cm 2 s −1 ) clearing more slowly than cytochrome c ( D = 1.63 × 10 −6 cm 2 s −1 ) . Albumin clearance studies were also performed with nonporous membranes and the results showed that the outlet concentration matched that of the inlet indicating that no albumin was lost to the system (e.g., by adsorption).…”

Section: Resultsmentioning

confidence: 98%

“…The results are shown in Figure 3b where it is shown that as the diffusion coefficient decreases, so does the clearance with FITC-insulin (D = 8.2 × 10 −7 cm 2 s −1 ) [25] clearing more slowly than cytochrome c (D = 1.63 × 10 −6 cm 2 s −1 ). [26] Albumin clearance studies were also performed with nonporous membranes and the results showed that the outlet concentration matched that of the inlet indicating that no albumin was lost to the system (e.g., by adsorption).…”

Section: Characterization Of Solute Clearance By 75 Nm Npn-omentioning

confidence: 99%

Second Generation Nanoporous Silicon Nitride Membranes for High Toxin Clearance and Small Format Hemodialysis

Hill

Walker

Salminen

et al. 2020

Adv Healthcare Materials

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Conventional hemodialysis (HD) uses floor‐standing instruments and bulky dialysis cartridges containing ≈2 m2 of 10 micrometer thick, tortuous‐path membranes. Portable and wearable HD systems can improve outcomes for patients with end‐stage renal disease by facilitating more frequent, longer dialysis at home, providing more physiological toxin clearance. Developing devices with these benefits requires highly efficient membranes to clear clinically relevant toxins in small formats. Here, the ability of ultrathin (<100 nm) silicon‐nitride‐based membranes to reduce the membrane area required to clear toxins by orders of magnitude is shown. Advanced fabrication methods are introduced that produce nanoporous silicon nitride membranes (NPN‐O) that are two times stronger than the original nanoporous nitride materials (NPN) and feature pore sizes appropriate for middle‐weight serum toxin removal. Single‐pass benchtop studies with NPN‐O (1.4 mm2) demonstrate the extraordinary clearance potential of these membranes (105 mL min−1 m−2), and their intrinsic hemocompatibility. Results of benchtop studies with nanomembranes, and 4 h dialysis of uremic rats, indicate that NPN‐O can reduce the membrane area required for hemodialysis by two orders of magnitude, suggesting the performance and robustness needed to enable small‐format hemodialysis, a milestone in the development of small‐format hemodialysis systems.

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The Effective Charge Number and Diffusion Coefficient of Cationic Cytochrome c in Aqueous Solution.

Cited by 16 publications

References 0 publications

Protein adsorption and activity on carbon xerogels with narrow pore size distributions covering a wide mesoporous range

Protein adsorption and activity on carbon xerogels with narrow pore size distributions covering a wide mesoporous range

Electrochemical behaviour of cytochrome c at electrically heated microelectrodes

Second Generation Nanoporous Silicon Nitride Membranes for High Toxin Clearance and Small Format Hemodialysis

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