Protein adsorption and activity on carbon xerogels with narrow pore size distributions covering a wide mesoporous range

Ramírez-Montoya, Luis A.; Concheso, A.; Alonso-Buenaposada, Isabel D.; García, Héctor A.; Menéndez, J.Á.; Arenillas, A.; Montes-Morán, Miguel A.

doi:10.1016/j.carbon.2017.04.009

Cited by 14 publications

(18 citation statements)

References 48 publications

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“…It is quite obvious, however, that, in spite of the narrow pore distributions, these materials cannot be considered ordered mesoporous solids [51]. isotherms exhibit a point of intersection at C e~0 .17 mg cm -3 , which is relevant in relation to our previous work [46], in which it was stated that CX-30 had a similar adsorption capacity to CX-15 at pH=6 ( Fig. 5 in [46]).…”

Section: Equilibrium Resultsmentioning

confidence: 64%

“…The robustness of the kinetic model proposed here was tested using experimental data obtained from the adsorption of proteins on organic (OXs) and carbon xerogels (CXs). These materials have been singled out as excellent candidates for studying the interaction of biomolecules with porous materials, specifically with the aim of determining the effect of pore size on their adsorption and activity [46]. The porous structure of RF xerogels has been extensively reported [44][45][46]51].…”

Section: Equilibrium Resultsmentioning

confidence: 99%

“…These materials have been singled out as excellent candidates for studying the interaction of biomolecules with porous materials, specifically with the aim of determining the effect of pore size on their adsorption and activity [46]. The porous structure of RF xerogels has been extensively reported [44][45][46]51]. The nanostructure of these materials is essentially composed of packed quasi-spherical nodules that can be observed by SEM and TEM [51,52].…”

Section: Equilibrium Resultsmentioning

confidence: 99%

“…The voids between the spheres constitute the porosity of the materials. Accordingly, if the size of the nodules is narrowly controlled during the synthesis, the resulting pore size distribution must also be relatively narrow [44][45][46]. It is quite obvious, however, that, in spite of the narrow pore distributions, these materials cannot be considered ordered mesoporous solids [51].…”

Section: Equilibrium Resultsmentioning

confidence: 99%

“…In the present work we propose and solve a load-dependent surface diffusion model for protein adsorption that follows Fick´s second law in which (a) surface diffusivity depends on surface coverage (load) via a new empirical relation and (b) external liquid-film mass transfer resistance is considered. The model has been tested in the adsorption of cytochrome c onto mesoporous organic and carbon xerogel particles synthesized by means of microwave heating [44][45][46].…”

Section: Introductionmentioning

confidence: 99%

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Load-dependent surface diffusion model for analyzing the kinetics of protein adsorption onto mesoporous materials

Marbán

Ramírez-Montoya

García

et al. 2018

Journal of Colloid and Interface Science

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The adsorption of cytochrome c in water onto organic and carbon xerogels with narrow pore size distributions has been studied by carrying out transient and equilibrium batch adsorption experiments. It was found that equilibrium adsorption exhibits a quasi-Langmuirian behavior (a g coefficient in the Redlich-Peterson isotherms of over 0.95) involving the formation of a monolayer of cyt c with a depth of ∼4nm on the surface of all xerogels for a packing density of the protein inside the pores of 0.29gcm. A load-dependent surface diffusion model (LDSDM) has been developed and numerically solved to fit the experimental kinetic adsorption curves. The results of the LDSDM show better fittings than the standard homogeneous surface diffusion model. The value of the external mass transfer coefficient obtained by numerical optimization confirms that the process is controlled by the intraparticle surface diffusion of cyt c. The surface diffusion coefficients decrease with increasing protein load down to zero for the maximum possible load. The decrease is steeper in the case of the xerogels with the smallest average pore diameter (∼15nm), the limit at which the zero-load diffusion coefficient of cyt c also begins to be negatively affected by interactions with the opposite wall of the pore.

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Section: Equilibrium Resultsmentioning

confidence: 64%

Section: Equilibrium Resultsmentioning

confidence: 99%

Section: Equilibrium Resultsmentioning

confidence: 99%

Section: Equilibrium Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Load-dependent surface diffusion model for analyzing the kinetics of protein adsorption onto mesoporous materials

Marbán

Ramírez-Montoya

García

et al. 2018

Journal of Colloid and Interface Science

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Improved L‐Asparaginase Properties and Reusability by Immobilization onto Functionalized Carbon Xerogels

Barros,

Cristóvão,

Carneiro

et al. 2024

ChemPlusChem

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Enzyme immobilization can offer a range of significant advantages, including reusability, and increased selectivity, stability, and activity. In this work, a central composite design (CCD) of experiments and response surface methodology (RSM) were used to study, for the first time, the L‐asparaginase (ASNase) immobilization onto functionalized carbon xerogels (CXs). The best results were achieved using CXs obtained by hydrothermal oxidation with nitric acid and subsequent heat treatment in a nitrogen flow at 600 ºC (CX‐OX‐600). Under the optimal conditions (81 min of contact time, pH 6.2 and 0.36 g/L of ASNase), an immobilization yield (IY) of 100% and relative recovered activity (RRA) of 103% were achieved. The kinetic parameters obtained also indicate a 1.25‐fold increase in the affinity of ASNase towards the substrate after immobilization. Moreover, the immobilized enzyme retained 97% of its initial activity after 6 consecutive reaction cycles. All these outcomes confirm the promising properties of functionalized CXs as support for ASNase, bringing new insights into the development of an efficient, and stable immobilization platform for use in the pharmaceutical industry, food industry, and biosensors.

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Organic and Carbon Gels: From Laboratory to Industry?

Arenillas

Menéndez

Reichenauer

et al. 2019

Advances in Sol-Gel Derived Materials and Technologies

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Protein adsorption and activity on carbon xerogels with narrow pore size distributions covering a wide mesoporous range

Cited by 14 publications

References 48 publications

Load-dependent surface diffusion model for analyzing the kinetics of protein adsorption onto mesoporous materials

Load-dependent surface diffusion model for analyzing the kinetics of protein adsorption onto mesoporous materials

Improved L‐Asparaginase Properties and Reusability by Immobilization onto Functionalized Carbon Xerogels

Organic and Carbon Gels: From Laboratory to Industry?

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