The effect of water with modified isotope content on the parameters of free radical oxidation in vivo

Aa, Basov; Mg, Baryshev; Ss, Dzhimak; Im, Bykov; Ri, Sepiashvili; Pavliuchenko,

doi:10.15407/fz59.06.049

Fiziol Zh

2014

DOI: 10.15407/fz59.06.049

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The effect of water with modified isotope content on the parameters of free radical oxidation in vivo

Basov Aa¹,

Baryshev Mg²,

Dzhimak Ss³

et al.

Abstract: Влияние воды с модифицированным изотопным составом на показатели свободнорадикального окисления in vivo С помощью ядерного магнитного резонанса изучено влияние воды с модифицированным изотопным составом на содержание дейтерия в крови, проведена оценка состояния прооксидантно-антиоксидантной системы в крови и лиофилизированных тканях (печень, почки) под длительном ее в условиях моделированного окислительного стресса. Рассмотрены возможные механизмы реализации прямого и косвенного антиоксидантного эффекта этой в… Show more

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Cited by 6 publications

(2 citation statements)

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“…Investigations performed on different living organisms [6][7][8][9] have revealed predominantly positive effects. However, as in the case of heavy water, the effects were dose dependent [3,10,11], the choice of a bioobject being of great significance.…”

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confidence: 99%

The effect of water isotopic composition on Rhodococcus erythropolis biomass production

et al. 2015

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The effect of the physical and chemical properties of a medium on the functioning of biological systems is today attracting the close attention of researchers. Broadening the problem area in this field is related to many unsettled questions appeared upon analysis of the results of mild exposures of living matter of differ ent levels of organization. Isotopic composition of water is one of the most important physical and chem ical factors, the significance of which is doubtless [1,2]. It has been found that the biological effects could be observed upon small natural variations in the isoto pic composition of water both toward a decrease and increase with respect to the standard ocean water [2]. As a result of numerous studies of the effect of water with increased deuterium content on biological sys tems, different positive and negative dose and object dependent effects have been observed [1,[3][4][5][6].At present, research on the biological effects of water with decreased deuterium content has consider ably progressed. Investigations performed on different living organisms [6-9] have revealed predominantly positive effects. However, as in the case of heavy water, the effects were dose dependent [3, 10, 11], the choice of a bioobject being of great significance.The study of mechanisms of the effect of isotopic composition of water on the functioning of biological systems demonstrated their intricacy and diversity. Suggestions regarding the effect of deuterium on sys tems of hydrogen bonds providing the structure and functions of macromolecules were made. It was noted that hydrogen bonds with deuterium involved are sev eral times stronger than the conventional ones. Upon cell incubation in water with a changed D/H ratio, when compared to the initial one, not only the ratio of deuterium and protium water as a solvent within the biological system changed, but also isotopic exchange in hydroxyl, sulfhydryl, carboxyl, and amino groups of molecules of all the organic compounds occurred [1,[12][13][14].In connection with the growing number of studies of the biological effects of light water, investigation of the effect of such media on bacteria, including rhodo cocci, is of interest. This group of microorganisms is evolutionarily adapted to utilization and transforma tion of a wide range of compounds of hydrocarbon ori gin. Natural compounds utilized by actinobacteria including representatives of the Rhodococcus genus are petroleum hydrocarbons entering the biosphere in a natural and technogenic way and other natural biolog ically stable compounds including hydrophobic ones. The main habitat of actinobacteria is soils, humic and other organic compounds of which serve as an almost exhaustless carbon depot available for maintenance of the vital functions of this group of bacteria. To follow the ecological K strategy typical for rhodococci, they have a corresponding catabolic enzymatic apparatus and system of energy efficient transport of hydrocar bon molecules into the cell represented by the produc tion of biogenic surfac...

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The effect of water isotopic composition on Rhodococcus erythropolis biomass production

et al. 2015

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“…As a rule, inflammatory reactions in the biliary ducts are attributed to two predisposing anatomical and physiological conditions: bile stasis and eventual local infections. Specific role the enzyme link of the antioxidant system plays when protecting the body in inflammatory processes is attributed to the neutralization of free radicals and reactive molecules the formation of which is greatly increased under condition of oxidative stress [14]. Irrelevant functioning of antioxidant enzymes may lead to progressive pathological process and increase the frequency of unfavorable outcomes in the postoperative period.…”

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Change of parameters in prooxidant-antioxidant bile system in patients with the obstruction of bile-excreting ducts

Ilyitch¹,

Басов²

2015

Medical news of the North Caucasus

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The paper found that among the patients with choledocholithiasis and malignant neoplasms of biliopancreatoduodenalis area in the development of cholangitis it is observed a significant reduction in the capacity of the antioxidant system at the local level (in bile) by 78.3% and 72.9%, respectively. The paper showed that for the evaluation of the degree of decompensation in antiradical protection in biliary obstruction and inflammatory complications it is necessary to determine as additional indicators in bile the state level of the enzymes in the antioxidant system: the activity of catalase and superoxidedismutase that could improve the diagnosis in these pathological conditions and in addition would allow to appoint timely corrective actions and monitor the effectiveness of the therapy. The study showed that the catalase activity increased in the bile in patients with choledocholithiasis as compared to control values by 1.7 times in the absence of inflammatory complications and by 5.1 times-under cholangitis; in patients with malignant neoplasms of biliopancreatoduodenalis area its activity decreased by 49.4% while decreasing the area of maximum chemoluminescence by 42.2% and 72.5% respectively, whereas in the development of cholangitis the catalase activity increased by 4.5 times. In this case the increase in superoxidedismutase activity in all study groups was even more significant, indicating the adaptive changes, as well as the emerging imbalance in the enzymes of the 1st and 2nd line of the antioxidant system (mainly due to dismutase activity), which might be accompanied by a decrease in local nonspecific resistance, especially in inflammatory complications in patients with the studied nosologies.

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EPR spectroscopy solutions for assessment of decellularization of intrathoracic organs and tissues

Gubareva

Куевда

Джимак

et al. 2016

Dokl Biochem Biophys

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Using EPR spectroscopy it was established that the determination of the concentration of paramagnetic centers in lyophilized tissues allows indirect evaluation of the quality of decellularization of intrathoracic organs (diaphragm, heart, and lungs), since the content of paramagnetic particles in them can serve as a criterion of cell viability and points to the necessity to repeat decellularization. Experiments in rats showed that the EPR spectra of the native thoracic organs contained paramagnetic centers with g-factor values ranging from 2.007 to 2.011 at a concentration of 10(-8) to 6.62 × 10(-7) mol/g of lyophilized tissue, whereas in all decellularized tissues of the same organs paramagnetic particles were not detected.

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The effect of water with modified isotope content on the parameters of free radical oxidation in vivo

Cited by 6 publications

References 2 publications

The effect of water isotopic composition on Rhodococcus erythropolis biomass production

The effect of water isotopic composition on Rhodococcus erythropolis biomass production

Change of parameters in prooxidant-antioxidant bile system in patients with the obstruction of bile-excreting ducts

EPR spectroscopy solutions for assessment of decellularization of intrathoracic organs and tissues

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