The effect of water acidification on prolactin cells and pars intermedia PAS-positive cells in the teleost fish Oreochromis (formerly Sarotherodon) mossambicus and Carassius auratus

Bonga, S.E. Wendelaar; Meij, J.C.A. van der; Krabben, W. A. W. A. van der; Flik, G.

doi:10.1007/bf00219878

Cited by 19 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our initial observations on the effect of low water-pH on the PIPAS cells in goldfish were consistent with this hypothesis (Wendelaar Bonga et al 1984b). The activation of these cells, observed during the first two weeks of expo sure of the fish to acid water may represent an endocrine response to restore the marked drop in plasma calcium that immediately follows a reduction in water pH.…”

supporting

confidence: 81%

“…The fish were kept in well-aerated glass aquaria (8 fish per 100 1) at 20° C and a daily light period of 12 h. Four weeks before acidification of the water, two groups of 8 fish were adapted to high-calcium freshwater (CaCl2 in tap water) and two further groups of 8 fish to saline (NaCl in tap water). The composition of the water used has been reported earlier (Wendelaar Bonga et al 1984b). The Ca2 f concentration was 0.8 mM, the N a + concentration 1.9 mM and the pH 7.5.…”

Section: Methodsmentioning

confidence: 99%

“…However, no changes could be observed in the prolactin cells of gold fish (Wendelaar Bonga et al 1984a, b). Contrastingly, in goldfish the PAS-positive cells of the pars intermedia (fur ther termed PIPAS cells) became dramatically activated during exposure to acid water (Wendelaar Bonga et al 1984b). PIPAS cells are exclusively found in teleosts (Ball and Batten 1981).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Response of PAS-positive cells of the pituitary pars intermedia in the teleost Carassius auratus to acid water

1986

Self Cite

View full text Add to dashboard Cite

Summary. The PAS-positive or PIPAS cells in the pars in termedia of goldfish are activated after reduction of the pH of the ambient freshwater from 7.5 to 3.5. The cells increase in number and exhibit a five-fold increase in cell volume. Granular endoplasmic reticulum occupies most of the cytoplasm. Goldfish PIPAS cells (also termed calciumsensitive cells) are thought to have a hypercalcemic func tion. Therefore, their activation in acid water may be caused by the severe drop in plasma calcium concentration follow ing exposure of the fish to low water pH. However, activa tion of the PIPAS cells in response to acidification of the water is not prevented when the calcium concentration of the water is increased to levels that result in hypercalcemia instead of hypocalcemia. Activation of the PIPAS cells oc curs also in fish exposed to acidified freshwater enriched with NaCl to an osmolarity similar to that of the blood. This prevents the reduction in plasma osmolarity and Na ' and Cl" concentrations that follow exposure of goldfish to acidified normal freshwater. Our observations do not support the hypothesis that the PIPAS cells in goldfish pro duce a hypercalcemic hormone, or indeed any hormone involved in calcium metabolism or osmoregulation. The cells may be implicated in ¡icid-base regulation (a character istic of many types offish when exposed to acidified water) but the evidence is indirect.

show abstract

supporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Response of PAS-positive cells of the pituitary pars intermedia in the teleost Carassius auratus to acid water

1986

Self Cite

View full text Add to dashboard Cite

show abstract

“…Freshly dissected neurointermediate lobes were prefixed in 3% glutaraldehyde in 0.1 M sodium cacodylate (pH 7.4) for 15 min and fixed in a mixture of 3% glutaraldehyde in 0.1 M sodium cacodylate buffer, 2% osmium tetroxide (0 s 0 4), and 5% potassium dichromate (1:1:1) for 1-1.5 hr (Wendellar Bonga et al, 1984). After fixation the tissues were block stained by 2% uranyl acetate in water (1 hr).…”

Section: A T E R Ia L S and M E T H O D Smentioning

confidence: 99%

Morphology of the pars intermedia and the melanophore-stimulating cells in Xenopus laevis in relation to background adaptation

Rijk

Jenks

Bonga

1990

General and Comparative Endocrinology

View full text Add to dashboard Cite

The melanophore-stimulating hormone (MSH) cells of the amphibian pars intermedia secrete the peptide a-melanophore-stimulating hormone (a-MSH), which induces pigment dispersion in dermal melanophores. The purpose of the present study was to determine (1) whether prolonged activation of the secretory activity of the pars intermedia is associated with hypertrophy, hyperplasia, or both and (2) whether the MSH cells function as a homo geneous or heterogeneous population in meeting the physiological demand for MSH. The demand for MSH was manipulated by adapting animals for at least 3 weeks to white, two shades of grey, or black backgrounds. Morphometric analysis showed that the intermediate lobe volume was positively correlated with the degree of pigment dispersion in the mela nophores. The number of MSH cells per lobe was not affected by the degree of pigment dispersion. Therefore, we conclude that enlargement of the tissue associated with MSH cell activation involves hypertrophy rather than hyperplasia. Ultrastructural examination indi cated that the majority of MSH cells in black-adapted animals are biosynthetically active, whereas the cells of white-adapted animals are relatively inactive and show granule storage. The pars intermedia of grey-adapted toads contained both active and inactive cells, indi cating that MSH cells respond as a heterogeneous cell population in meeting the endocrine demand imposed by background. © 1990 Academic Press, inc.In general, activation of endocrine cells may involve hypertrophy, hyperplasia, or both. Furthermore, two patterns of cell re sponses can be distinguished during activa tion: either all cells become activated or only subpopulations of cells become acti vated. An example of the former are the oxytocin-producing neuroendocrine cells that respond as a homogeneous population to the suckling stimulus (Poulain and Wakerley, 1982). An example of the latter are the vasopressin-producing neuroendocrine cells that respond as a heterogeneous pop ulation to osmotic stress (Poulain and Wakerley, 1982). Morphometric and biochemi cal evidence has been advanced that the rat gonadotropes and lactotropes (Lucque et al., 1986) as well as the p cells of the pancreatic islets (Schuit et al., 1988) and thyroid follicular cells in mice (Gerber et al., 1987) form heterogeneous populations with regard to their secretory response to demand for hormone.In the present investigation we studied the response of amphibian melanophorestimulating hormone (MSH)-producing cells to a physiological stimulus. Two major questions were addressed. First, does the activation of the pars intermedia involve hypertrophy, hyperplasia, or both? Second, do the endocrine cells of this tissue function as a homogeneous or as a heterogeneous cell population in response to a demand for MSH? The MSH cell of Xenopus laevis provides a good model to study these ques tions because the pars intermedia activity can be easily manipulated. The MSH cells of animals placed on a black background release a-MSH, which stimulates pigment ...

show abstract

“…The proliferative changes of mucous cells with slight degenerative changes in the epidermis observed in the present study indicate a reactive phase (Fig.8) which constitutes a general response to stress conditions (Wendelaar Bonga et al 1984). Melanin accumulation together with leukocyte infiltration that constitute a defense barrier against microorganisms in front of which the skin can be more susceptible (Bols et al 2001).…”

Section: Discussionmentioning

confidence: 50%

Acute toxicity and histopathological alterations of Roundup® herbicide on "cachama blanca" (Piaractus brachypomus)

2008

View full text Add to dashboard Cite

Acute toxicity of the glyphosate -N (phosphonomethyl) glycine- herbicide, Roundup®, in juveniles of cachama blanca, (Piaractus brachypomus), was evaluated and the histopathological lesions were assessed. The 96 h lethal concentration 50 was 97.47mg.L-1 (P<0.05). In the gill, necrotic and proliferative lesions were detected. In the liver, congestion, degenerative foci, hyaline droplets and lipidic vacuolization of the hepatocytes were observed. In the stomach mild hyperplasia of mucous cells was detected, which was also observed in the skin. In this latter tissue, a large increase in the thickness of the epidermis with necrotic lesions, infiltration of leukocytes and melanin pigment were observed. In the brain, degenerative foci of neuronal bodies in the telencephalon associated with gliosis and infiltration of eosinophilic granule cells/mast cells were shown. In conclusion, gills, liver, skin and brain are susceptible to Roundup®. Moreover, effects on the central nervous system could affect olfaction as well as individual and group behavior, the reproductive performance of the fish and hence have repercussions at the population level.

show abstract

The effect of water acidification on prolactin cells and pars intermedia PAS-positive cells in the teleost fish Oreochromis (formerly Sarotherodon) mossambicus and Carassius auratus

Cited by 19 publications

References 25 publications

Response of PAS-positive cells of the pituitary pars intermedia in the teleost Carassius auratus to acid water

Response of PAS-positive cells of the pituitary pars intermedia in the teleost Carassius auratus to acid water

Morphology of the pars intermedia and the melanophore-stimulating cells in Xenopus laevis in relation to background adaptation

Acute toxicity and histopathological alterations of Roundup® herbicide on "cachama blanca" (Piaractus brachypomus)

Contact Info

Product

Resources

About