The Effect of Various Wound Dressings on the Activity of Debriding Enzymes

Abstract

Antimicrobial dressings are widely used for management of wound bioburden. Frequently, they are used in combination with other topical therapeutic drugs, such as enzymatic debriding agents for the removal of wound necrotic tissues. Such combined applications may have greater potential to achieve multiple healing activities simultaneously, including exudate and bioburden management, debridement, and tissue regeneration. Overall, the authors' testing found that collagenase was observed to be more tolerant when used with the dressings tested than papain. These findings merit further exploration in clinical wounds to confirm clinical validity.

Study on the Antimicrobial Properties of Citrate-Based Biodegradable Polymers

“…CUPE and BPLP performed intermediate inhibition strength against microorganisms, where the differences were still significant with around 20 and 50% reduction for E. coli and S. aureus , respectively, at day 28 ( p  < 0.01). In comparison, the commercially available wound dressing, Hydrofera Blue, showed no antimicrobial property at the early time points, but gradually activated its germicidal function at the late time points due to the slow release of antimicrobial dyes, methylene blue, which is incorporated within Hydrofera Blue (Shi et al, 2010). Most importantly, Figure 4 shows that the bacteria turbidity incubated with POC scaffolds reduced 68% for E. coli and 83% for S. aureus after 28 h of incubation compared to free bacteria control indicating that POC has similar or better bacteria-killing properties compared to that of free citric acid (29 mg/ml) and commercial Hydrofera Blue samples at late time points.…”

Collagenase for Enzymatic Debridement

“…27 For example, antimicrobial dressings containing ionic silver produces minimal inhibition of collagenase activity, while silver dressings reduced its activity by more than 50% and cadexomer iodine inhibited its activity by approximately 90%. 28 In addition, pH levels below 6.0 or above 8.0 found in some antiseptic cleansers containing heavy metal ions, acetic acid, or hyperchlorite also reduce the biologic activity of collagenase and should be avoided or removed from the wound bed via cleansing with saline before collagen is applied.…”

Viability of Intratubular Bacteria after Chemomechanical Caries Removal

“…Although Papacarie gel (pH = 9.2) is less alkaline than Cariosolv gel (pH = 10.1), it contains the papain enzyme (similar to human pepsin), which has been considered as a potent antibacterial and anti-inflammatory agent (24). Papain is widely used in the medical field as a wound healing dressing (25) and in the treatment of tumors (26). It destroys peptidoglycans, which cover the bacterial cell wall, leading to exposure of the cell membrane as a result of osmotic shock and exudation of the cytoplasmic contents (27).…”