The effect of various inflammatory agents on the alternative metabolic pathways of arginine in mouse and rat macrophages

Abstract

The activity and expression of NOS II and arginase can be stimulated in peritoneal macrophages in vitro by injecting inflammatory agents into the peritoneal cavity. A markedly different response in arginine metabolism was observed in mouse and rat macrophages. Casein treatment was a potent inducer for both enzymes. NDV vaccines induced mainly NOS II, while thioglycollate induced arginase.

The effect of various inflammatory agents on the phagocytosis and cytokine profile of mouse and rat macrophages

“…CD-CR 1 male mice (30-35 g) and Wistar male rats (200-250 g) were injected i.p. with thioglycollate broth (prepared according to the prescription of the manufacturer, Difco) and with dephosphorylated casein [20,32]. 1 ml thioglycollate or casein was injected into mice, 5 ml were injected into rats.…”

“…1 ml thioglycollate or casein was injected into mice, 5 ml were injected into rats. These doses were based on our preliminary experiments detailed in our previous tests [20]. Carrageenan (0.4 w/v% in PBS, kept at 37 °C, to prevent agglutination) was prepared immediately before use and 0.5 ml and 2.5 ml were injected into mice and rats, respectively.…”

“…Cell cultures 3h samples were tested for the studies, because arginase activity is well measurable after this period and it is not changed until 6 h and 24 h cultures were found the most favorable for nitrite and NOS measurements [15,20]. For this reason, in addition to the measurement of freshly adhered samples, 3 h and 24 h studies were chosen.…”

“…Formerly, we have published a comparative study, in this journal, in which the alterations of arginine metabolism (arginase and NOS) in various experimental infl ammations were investigated [20]. However, that study did not involve the measurements of pro-and anti-infl ammatory cytokines regulating the arginine metabolism of macrophages.…”

“…However, that study did not involve the measurements of pro-and anti-infl ammatory cytokines regulating the arginine metabolism of macrophages. In this paper, we describe a cytokine profi le for mouse and rat peritoneal macrophages previously induced by various infl ammatory challenges, including non-specifi c (thioglycollate [20,21], casein [20,22,23], carrageenan [24,25]) and immunologically specifi c (Bacillus Calmette-Guérin, BCG [26][27][28], and Newcastle Disease Virus, NDV [20,[29][30][31]) treatments. Another macrophage function, phagocytosis has also been compared in these differently challenged macrophages.…”