The effect of various inflammatory agents on the phagocytosis and cytokine profile of mouse and rat macrophages

Hrabák, A.; Bajor, Tamás; Csuka, I

doi:10.1007/s00011-007-7057-7

Cited by 14 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acute phase bone marrow response was measured by absolute leukocytes count and leukocytes count expressed as percentage. Phagocytic activity was assessed with the in vitro phagocytosis test, calculating two parameters: the phagocytic index (PI) (PI% = phagocytes with at least one phagocyted germ from 200 leukocytes counted) and the phagocytic activity (PA) (PA = number of germs phagocyted by 100 leukocytes) 26–28. The effects of the new compounds were compared with those from the inflammation group, and with those from the group treated with meloxicam as a reference NSAID.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis of New N‐Substituted 5‐Arylidene‐2,4‐thiazolidinediones as Anti‐Inflammatory and Antimicrobial Agents

Nastasă¹,

Tiperciuc²,

Pârvu³

et al. 2013

Archiv der Pharmazie

View full text Add to dashboard Cite

A novel series of 5-arylidene-2,4-thiazolidinediones (TZDs) 2a-p was synthesized from the condensation of 3-((2-phenylthiazol-4-yl)methyl)thiazolidine-2,4-dione with different benzaldehyde derivatives. All the structures were confirmed by their spectral (IR, ¹H NMR, ¹³C NMR and mass) and elemental analytical data. The new molecules were evaluated in vivo as anti-inflammatory agents in an acute experimental inflammation, evaluating the acute phase bone marrow response and phagocyte activity. All compounds, excepting one, reduced the absolute leukocytes count due to the lower neutrophil percentage. Phagocytary index was decreased by the same molecules, while only half of them reduced the phagocytary activity. The effect was superior to meloxicam, the reference anti-inflammatory drug, for the majority of the TZD derivatives. The new molecules were also investigated for their antimicrobial properties on Gram-positive and Gram-negative bacteria and one fungal strain. Two compounds (2e and 2n) manifested growth inhibition capacity on all the tested strains.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Investigation of phagocytic activity of PMN cell was performed as described in literature 26–28 with slight modifications. Blood samples from treated animals were incubated in siliconated tube with E. coli suspension at 37°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of New N‐Substituted 5‐Arylidene‐2,4‐thiazolidinediones as Anti‐Inflammatory and Antimicrobial Agents

Nastasă¹,

Tiperciuc²,

Pârvu³

et al. 2013

Archiv der Pharmazie

View full text Add to dashboard Cite

show abstract

“…The inflammatory process involves a complex and coordinated cascade of cellular and molecular events that culminate with production and release of several cytokines . Among these cytokines, IL‐6 is a proinflammatory cytokine that may activate and modulate specific cells and, therefore, exerts an important role in the inflammatory reaction and in the bone resorption .…”

Section: Discussionmentioning

confidence: 99%

Microparticulated and nanoparticulated zirconium oxide added to calcium silicate cement: Evaluation of physicochemical and biological properties

Silva

Bosso

Ferino

et al. 2014

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

The physicochemical and biological properties of calcium silicate-based cement (CS) associated to microparticulated (micro) or nanoparticulated (nano) zirconium oxide (ZrO 2 ) were compared with CS and bismuth oxide (BO) with CS. The pH, release of calcium ions, radiopacity, setting time, and compression strength of the materials were evaluated. The tissue reaction promoted by these materials in the subcutaneous was also investigated by morphological, immunohistochemical, and quantitative analyses. For this purpose, polyethylene tubes filled with materials were implanted into rat subcutaneous. After 7, 15, 30, and 60 days, the tubes surrounded by capsules were fixed and embedded in paraffin. In the H&E-stained sections, the number of inflammatory cells (ICs) in the capsule was obtained. Moreover, detection of interleukin-6 (IL-6) by immunohistochemistry and number of IL-6 immunolabeled cells were carried out. von Kossa method was also performed. The differences among the groups were subjected to Tukey test (p 0.05). The solutions containing the materials presented an alkaline pH and released calcium ions. The addition of radiopacifiers increased setting time and radiopacity of CS. A higher compressive strength in the CS 1 ZrO 2 (micro and nano) was found compared with CS 1 BO. The number of IC and IL-6 positive cells in the materials with ZrO 2 was significantly reduced in comparison with CS 1 BO. von Kossa-positive structures were observed adjacent to implanted materials. The ZrO 2 associated to the CS provides satisfactory physicochemical properties and better biological response than BO. Thus, ZrO 2 may be a good alternative for use as radiopacifying agent in substitution to BO.

show abstract

“…Two distinct parameters were calculated to characterize the activity of phagocytes: the phagocytic activity (PA) defined as the number of E. coli germs phacocyted by 100 leukocytes and the phagocytic index (PI%) defined as the percentage of leukocytes that phagocyted at least one germ [9,21,22].…”

Section: Methodsmentioning

confidence: 99%

The Effect of Some 4,2 and 5,2 Bisthiazole Derivatives on Nitro-Oxidative Stress and Phagocytosis in Acute Experimental Inflammation

et al. 2014

View full text Add to dashboard Cite

Nineteen bisthiazoles were tested in order to assess their anti-inflammatory and antioxidant properties. First, we evaluated the in vitro direct antioxidant capacity of the bisthiazoles using the DPPH radical scavenging method. Then, the anti-inflammatory effect was tested in acute rat experimental inflammation by measuring the acute phase bone marrow response, the phagocytic capacity and the serum nitro-oxidative stress status. Although none of the substances showed significant direct antioxidant potential in the DPPH assay, most of them improved serum oxidative status, when administered to rats with inflammation. Four of the bisthiazoles proved to have good anti-inflammatory properties, similar or superior to that of equal doses meloxicam.

show abstract

The effect of various inflammatory agents on the phagocytosis and cytokine profile of mouse and rat macrophages

Cited by 14 publications

References 42 publications

Synthesis of New N‐Substituted 5‐Arylidene‐2,4‐thiazolidinediones as Anti‐Inflammatory and Antimicrobial Agents

Synthesis of New N‐Substituted 5‐Arylidene‐2,4‐thiazolidinediones as Anti‐Inflammatory and Antimicrobial Agents

Microparticulated and nanoparticulated zirconium oxide added to calcium silicate cement: Evaluation of physicochemical and biological properties

The Effect of Some 4,2 and 5,2 Bisthiazole Derivatives on Nitro-Oxidative Stress and Phagocytosis in Acute Experimental Inflammation

Contact Info

Product

Resources

About