The effect of type II collagen on MSC osteogenic differentiation and bone defect repair

Bioactive glass induced osteogenic differentiation of human adipose stem cells is dependent on cell attachment mechanism and mitogen-activated protein kinases

“…p38 and JNK are typically thought to be activated mainly by cytokines and various environmental stresses but there is also evidence that they play a role as regulators of osteogenic differentiation (Chiu et al, 2014;Gu et al, 2015;Ivaska et al, 1999;Suzuki et al, 2002;Tominaga et al, 2005). The activation of JNK downstream target c-Jun was observed in response to both BaGs.…”

“…In addition to ERK1/2, MAPKs p38 and c-Jun N-terminal kinases (JNKs) are also tightly connected to the regulation of osteogenic differentiation. Both of them are known to be involved in both the cell attachment-related signalling cascades (Chiu et al, 2014;Ivaska et al, 1999) as well as in chemical stimuli induced osteogenesis (Gu et al, 2015;Suzuki et al, 2002;Tominaga et al, 2005).…”

“…In order to observe the osteogenic differentiation induced solely by the BaGs, no osteogenic supplements were used. Human ASCs were chosen due to their wellknown ability to differentiate towards osteoblastic phenotype in vitro and their successful utilisation in clinical bone TE-based treatments (Detsch et al, 2015;Haimi et al, 2009a;Haimi et al, 2009b;Larranaga et al, 2015;Marino et al, 2010;Ojansivu et al, 2015;Sandor et al, 2014;Waselau et al, 2012). To characterise the material properties possibly affecting cell behaviour, the roughness of the glasses as well as the dissolution and surface structures of the BaGs were studied.…”

Characterization of human ethmoid sinus mucosa derived mesenchymal stem cells (hESMSCs) and the application of hESMSCs cell sheets in bone regeneration

“…(Cyagen Biosciences Inc., Guangzhou, China). For the induction of hESMSCs, the osteo-, adipo-and chondro-inductive medium purchased from Invitrogen was added as previously reported [36]. For co-culture, hESMSCs were seeded onto the upper layer of transwells (Millipore, Billerica, MA) at a density of 2 Â 10 4 cells/well, and rBMSCs were cultured at the basal compartment of a 6-well plate.…”

“…For ALP staining, cells were fixed in 70% ethanol for 20 min, a BCIP/NBT Alkaline Phosphatase Color Development Kit (Beyotime Institute of Biotechnology, China) was used according to the manufacturer's instructions [36]. After underwent osteogneic induction or cocultured in osteogenic medium for 14 days, alizarin red-S (ARS) staining was performed as previously described, cells were fixed in 70% ethanol for 30 min, and stained with Alizarin Red S (Sigma) for 30 min at 37 C. For Oil red O staining, cells were fixed in 4% PFA for 15 min, then incubated with Oil-red O (Sigma) for 30 min at 37 C to assess lipid formation after 14 days' adipo-induction.…”