The effect of transforming growth factor beta one (TGF‐β₁) on wound healing, with or without barrier membranes, in a Class II furcation defect in sheep

Abstract

The purpose of this study was to analyse the effect of TFG-beta 1 on wound healing in standardized Class II furcation defects of 48 mandibular second premolar teeth in 24 sheep. The experimental design included a control group (carrier only, 25% pluronic F-127), and 2 experimental groups: group A (80 micrograms/ml TGF-beta 1 + carrier) and group B (80 micrograms/ml TGF-beta 1 + carrier covered with a barrier membrane). Sheep were killed either 2 wk or 6 wk after surgery. Mesiodistal sections of the decalcified specimens were quantified histologically using stereology. Percentage volumes of regenerated bone, fibrous connective tissue and cementum were calculated for each furcation defect. Mean values were analysed using multiple ANOVA; p values were calculated using paired and unpaired Student's t-tests. After 2 wk there was more bone in group B than either of the other 2 groups, but this was not statistically significant. By 6 wk more bone was present in group A than in the control group (p < 0.02) and also in group B when compared with both group A and the control group (p < 0.02 and p < 0.44), respectively. In the 4 wk between sampling significantly more bone had formed (group A < 0.05 and group B p < 0.003, respectively). A negative correlation existed between volumes of bone and fibrous connective tissue and no significant differences between the volumes of cementum were evident between any of the groups. This study demonstrated that TGF-beta 1 encouraged bone regeneration in Class II furcation defects in sheep, an effect enhanced by the presence of a barrier membrane. This is the first report on the use of TGF-beta 1 in conjunction with GTR in periodontal defects.

Stimulation of growth factor synthesis in skin wounds using tissue extract (G‐90) from the earthworm Eissenia foetida

“…33,34 Treatment of wounds with nanogram quantities of TGF-, resulted in significant differentiation of structures and a decrease in the size of wound areas. 35,36 Significant proliferation of epithelial cells detected in wounds treated with G-90 by histological analysis was probably a consequence of induced growth factors (EGF, FGF). From our results we conclude that G-90 is a valuable mixture of biologically active macromolecules, that in nanogram quantities increase proliferation of cells, and may therefore accelerate wound healing.…”

Periodontal repair in dogs: effect of transforming growth factor‐β1 on alveolar bone and cementum regeneration

“…Ripamonti et al (1996) reported that doses from 5 to 100 mg of rhTGF-b1 in a collagen matrix carrier provided rather limited bone regenera-tion after 30 days in nonhuman primate critical size calvarial defects. Mohammed et al (1998) evaluated purified monkey TGF-b1 in sheep class II furcation defects and found that at 2 weeks, TGF-b1 plus carrier sites did not differ from carrier controls. At 6 weeks, TGF-b1 plus carrier sites had significantly more bone than carrier alone; nevertheless, in the 6-week TGF-b1 specimens the increase over controls was only 11% of the total defect volume.…”

Periodontal Tissue Regeneration Using Fibroblast Growth Factor -2: Randomized Controlled Phase II Clinical Trial

“…Some researchers are already trying to establish new treatments to accelerate the regeneration of periodontal tissue by local application of human recombinant cytokines to stimulate proliferation and differentiation into hard-tissue forming cells of undifferentiated mesenchymal cells among periodontal ligament cells. Direct local application of a combination of factors such as platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF)-I [6], bone morphogenetic protein (BMP)-2 [7], [8], transforming growth factor (TGF)-β [9], osteogenic protein (OP)-1 [10] and brain-derived neurotrophic factor (BDNF) [11] to artificial defects in periodontal tissue made in laboratory animals reportedly stimulates and promotes regeneration of regional periodontal tissue. In addition, the efficacy of PDGF-BB plus β-tricalcium phosphate (β-TCP, an osteoconductive scaffold) for periodontal tissue regeneration in human has recently been reported [12].…”