The effect of total inorganic nitrogen and the balance between its ionic forms on adventitious bud formation and callus growth of ‘Węgierka Zwykła’ plum (Prunus domestica L.)

Nowak, Barbara; Miczyński, Kazimierz; Hudy, Ludwik

doi:10.1007/s11738-007-0058-x

Cited by 23 publications

(31 citation statements)

References 32 publications

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“…The highest multiplication rate of A. polyphylla was achieved with intermediate concentrations of NH 4 ? and NO 3 -, namely ratios 20:40, 30:30 and 40:20, and decreased significantly outside these limits. Our results parallel those obtained in other studies (Avila et al 1998;Tsay 1998;Ramage and Williams 2002;Nowak et al 2007). Lowering the NH 4 ?…”

Section: Discussionsupporting

confidence: 93%

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Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Ivanova

Staden

2009

Plant Cell Tiss Organ Cult

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The role of nitrogen (N) supplied as inorganic ammonium (NH 4 ? ) and nitrate (NO 3 -), and organic glutamine on shoot regeneration, incidence of hyperhydricity and production of good quality shoots in Aloe polyphylla explants was investigated. The omission of total N from the culture medium resulted in low proliferation and hindered shoot growth. Ammonium as the sole source of N depressed shoot regeneration and growth and escalated the frequency of hyperhydricity to ca. 50%. When NO 3 -was used as the sole N source, shoots of good quality were produced and hyperhydricity was completely eliminated. Overall, the MS N combination was superior to any single N source for proliferation and growth of shoots, suggesting a synergistic effect between NH 4 ? and NO 3 -on shoot regeneration. Moreover, the relative amounts of NH 4 ? and NO 3-also influenced the parameters tested. The highest regeneration was obtained with NH 4 ? :NO 3 -ratios (mM) of 20:40, 30:30 and 40:20. Decreasing the ratio of NH 4 ? :NO 3 -lowered the incidence of hyperhydricity. This study also demonstrates the potential of glutamine as the sole source of N, since its application resulted in the production of good quality shoots and almost no hyperhydricity.

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Section: Discussionsupporting

confidence: 93%

“…Conversely, in our experimental system, increasing the total N led to a proportional increase in the multiplication rate, which was not at the expense of shoot growth and quality. Similar results were reported for Prunus domestica (Nowak et al 2007).…”

Section: Discussionsupporting

confidence: 90%

Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Ivanova

Staden

2009

Plant Cell Tiss Organ Cult

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“…All these alterations presumably enhanced and produced a balanced basal medium composition that was high suitable for callus formation. Obtaining a balanced medium composition was also necessary to successfully establish an in vitro culture of Gatharanthus roseus (Morard and Henry 1998), callus growth of Prunus domestica L. (Nowak et al 2007), regeneration of Dianthus henteri (Cristea et al 2010), and callus induction and regeneration of Foeniculum vulgare (Khorami and Safarnejad 2011). Specific basal medium for callus initiation in anther culture was also reported on other plants.…”

Section: Discussionmentioning

confidence: 99%

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

Winarto¹,

Rachmawati²,

Silva

2011

Plant Growth Regul

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A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1-1.0 mg/l), a-naphthalene acetic acid (0.01-0.2 mg/l), thidiazuron (0.5-2.0 mg/l), 6-benzylaminopurine (0.5-1.0 mg/l), and kinetin (0.5-1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto-Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto-Teixeira-3. Shoots formed a strong adventitious root system on New Winarto-Teixeira-3 containing 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14-18), 15 aneuploid (n = 20-26), 126 diploid (n = 28-34) and 5 triploid (n = 45-57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

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“…As a consequence of potassium uptake, nitrate use increases, promoting growth. Potassium nitrate was found to be important in the germination of plum (Prunus domestica L.) 27 while some banana has a preference for NH 4 NO 3 28 . MS medium has a high concentration of NH 4 NO 3 , which is a source of NH Nitrate-N induces the activity of nitrate reductase enzyme that reduces nitrate to ammonium, which is then incorporated into proteins 29,30 .…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the form of inorganic nitrogen and ammonium to nitrate ratio has been reported to greatly affect the growth and differentiation of cultured tissues 31,32 . Nevertheless, with a high NH + 4 , the uptake of cations is reduced and replaced instead by the uptake of an anion such as phosphorus with an accompanying release of an H + equivalent, which could lead to a more acidic pH and could be toxic to cells 27 . Glycine, present in MS medium, is also a source of organic nitrogen which has been reported to enhance plant regeneration in maize 33 .…”

Section: Discussionmentioning

confidence: 99%

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Thawaro¹,

Te-chato²

2010

ScienceAsia

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Nutritional requirements for germination of mature zygotic embryos of four genotypes of Elaeis guineensis Jacq. Dura × Pisifera were assessed. A factorial experiment was conducted with four genotypes from separate crossing events and three culture media to show Interactions between genotypes and culture media on seedling germination (p < 0.05). The highest frequency of normal seedling germination was facilitated by 1/2 Murashige and Skoog (MS) medium (29.2%), followed by MS (15.0%) and Blaydes (12.9%). Speed of shoot formation followed a similar trend. Roots were induced most rapidly in 1/2 MS medium, followed by Blaydes and then MS. Root induction was similar in 1/2 MS medium (2.29%) and Blaydes medium (1.25%) but only significantly higher than in MS (0.00%).

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The effect of total inorganic nitrogen and the balance between its ionic forms on adventitious bud formation and callus growth of ‘Węgierka Zwykła’ plum (Prunus domestica L.)

Cited by 23 publications

References 32 publications

Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

Nitrogen source, concentration, and NH4 +:NO3 − ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

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