The effect of tobacco smoking and of betel chewing with tobacco on the buccal mucosa: a cytomorphometric analysis

Ramaesh, Thaya; Mendis, B. R. R. N.; Ratnatunga, N; Thattil, R. O.

doi:10.1111/j.1600-0714.1999.tb02108.x

Cited by 52 publications

(54 citation statements)

References 25 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in the buccal cells of individuals with malignant lesions 11,13 or of smokers, 12,15 alterations distinct from those in the present study were found. In individuals with a tobacco-chewing habit and in those with smoking and tobacco-chewing habits combined, an increase in nuclear diameter and a decrease in cell diameter were observed, 15 as were seen in samples of individuals with tumors in the mouth floor.…”

Section: Discussioncontrasting

confidence: 81%

“…In individuals with a tobacco-chewing habit and in those with smoking and tobacco-chewing habits combined, an increase in nuclear diameter and a decrease in cell diameter were observed, 15 as were seen in samples of individuals with tumors in the mouth floor. 13 Cowpe et al 11 did not find size changes in the nucleus in samples of suspicious lesions of the buccal mucosa and the mouth floor but did observe a decrease in cytoplasmic area in lesions of the buccal mucosa.…”

Section: Discussionmentioning

confidence: 93%

“…Fifty cells on each slide were selected randomly for examination. [11][12][13][14] Areas with cells folded over and/ or clumped were avoided 15 because of the difficulty involved in determining cell boundaries. The image of the cytologic fields was captured at a magnification of 400 times ( Figures 3A through E) with the use of a Sony CCD Iris Color Video camera, model DXC-107A (Sony Electronics Inc, Tokyo, Japan).…”

Section: Cytomorphometric Analysismentioning

confidence: 99%

See 2 more Smart Citations

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Pereira

Tanaka

Lima

et al. 2009

The Angle Orthodontist

View full text Add to dashboard Cite

Objective: To test the null hypothesis that metal and ceramic brackets have no effect on the epithelial cells of the buccal mucosa. Materials and Methods: Two metal and two ceramic brackets were bonded in 21 individuals of both sexes. With the use of liquid-based exfoliative cytology, morphometric and morphologic changes in buccal mucosa cells adjacent to these brackets were determined and were compared at three time points: baseline (T0), 60 days after placement (T1), and 30 days after removal of the brackets (T2). Results: A decrease in nuclear area and an increase in cytoplasmic area occurred in the buccal mucosa cells adjacent to the brackets at T1 (P Ͻ .01). At T2, this altered morphometry persisted only in cells adjacent to the metal brackets, although to a lesser degree than at T1 (P Ͻ .01). A greater decrease in nuclear area was noted in cells adjacent to the metal brackets than in those next to the ceramic brackets (P Ͻ .01). At T0, the proportions of surface and subsurface cells were similar, but at T1, a predominance of surface cells was observed (P Ͻ .05). At all time points, smears of cells appeared normal or normal with some inflammatory changes. Conclusion:The hypothesis is rejected. Placement of metal and ceramic brackets in the buccal cavity induces cellular alterations. These alterations do not suggest malignancy. (Angle Orthod. 2008;00:373-379.)

show abstract

Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 93%

Section: Cytomorphometric Analysismentioning

confidence: 99%

See 1 more Smart Citation

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Pereira

Tanaka

Lima

et al. 2009

The Angle Orthodontist

View full text Add to dashboard Cite

show abstract

“…Several studies have reported the effect of different types of diseases and aggressive agents on oral mucosa, such as alcohol, and tobacco 10,12,[20][21][22][23][24][25] . However, studies reporting physical trauma associated to use of orthodontic appliances are still scarce 8 .…”

Section: Resultsmentioning

confidence: 99%

Preclinical Alterations of Oral Epithelial Cells in Contact With Orthodontic Appliances

Arruda¹,

Trevilatto²,

Camargo³

et al. 2011

BIOMED PAP

View full text Add to dashboard Cite

Aim. This study evaluated the behavior of oral epithelial cells in contact with orthodontic appliances. Methods. Oral epithelial cells of clinically normal buccal mucosa were obtained by liquid-based exfoliative cytology from 22 orthodontic patients. The following regions were evaluated: a) oral mucosa exposed to friction from orthodontic brackets; b) oral mucosa exposed to friction from the tube on the orthodontic band, and c) oral mucosa not exposed to friction (control area). Nuclear (NA) and cytoplasmic (CA) areas and NA/CA ratio were assessed by an image analysis system. Cell morphology and cellularity were also analyzed by Papanicolaou technique.Results. The NA of the cells in contact with orthodontic brackets and bands were smaller than the control area. Cells in contact with the brackets showed the greatest reduction in CA in comparison with the cells subjected to friction from the tube, and the control group. Smears classified as type I predominated in all regions analyzed, although type II were predominant, together with superficial epithelial cells, mainly in the oral mucosa in contact with the band tube.Conclusion. Preclinical alterations in the epithelial cells of oral mucosa, caused by orthodontic appliances, are reduction in NA, increase in cell keratinization and inflammatory features, especially in the band tube area.

show abstract

“…These include radiotherapy, smoking, alcohol, and malignant oral lesions. Therefore, the effects of such factors, if present, should be taken into account when assessing a lesion under investigation (19)(20)(21).…”

Section: Discussionmentioning

confidence: 99%