The effect of the timing of prenatal X-irradiation on Purkinje cell numbers in rat cerebellum

Li, Hong Peng; Miki, Takanori; He, Gu; Satriotomo, Irawan; Mastumoto, Yoshiki; Kuma, Hiromi; Gonzalez, Daniel; Bedi, K. S.; Sekiya, Hiroshi; Takeuchi, Yoshiki

doi:10.1016/s0165-3806(02)00542-4

Cited by 23 publications

(19 citation statements)

References 37 publications

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“…These data suggest that the decreased number of PCs during prenatal neurogenesis was the primary cause of the formation of simple folia without secondary folds. Prenatal X-irradiation at E14 and E15, which was accompanied by PC loss, produced changes in lobular patter of cerebella that are comparable to our observations (Li et al, 2002a). Furthermore, altered patterns of fissure formation and foliation due to loss of the PC did also occur in engrailed-2 mouse mutants (Baader et al, 1998).…”

Section: Cerebellar Patterning Is Affected By Exposure To Brdusupporting

confidence: 87%

“…Altered patterns of fissure formation and foliation did also occur in lobules VIII and IX of engrailed mouse mutants and the X-irradiated rats (Millen et al, 1994;Baader et al, 1998;Li et al, 2002a), suggesting the existence of a particular vulnerability of lobular patterning in this region of the posterior lobe. Moreover, the PCs of these posterior lobules form part of a transverse band characterized by expression of zonal specific markers (Ozol et al, 1999).…”

Section: Cerebellar Patterning Is Affected By Exposure To Brdumentioning

confidence: 99%

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Bromodeoxyuridine administered during neurogenesis of the projection neurons causes cerebellar defects in rat

Sekerková

Ilijić

Mugnaini

2004

J of Comparative Neurology

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Bromodeoxyuridine (BrdU) is broadly used in neuroscience to study embryonic development and adult neurogenesis. The potential toxicity of this halogenated pyrimidine analogue is frequently neglected. In this study, we administered BrdU in small doses by the progressively delayed cumulative labeling method to immunocytochemically tag different cerebellar cell types with antibodies to specific markers and BrdU in the same section. The well-known structure of the cerebellum made it possible to ascertain several toxic effects of the treatment. Time-pregnant rats were given five or six injections of 5 or 6 mg of BrdU ( approximately 12-20 mg/kg) at 8-hour intervals over 2 successive days between day 11 and 21 of pregnancy (E11-E12 to E20-E21), and the adult progeny was processed by immunocytochemistry. We demonstrate that this treatment effectively labeled distinct cerebellar cell populations but produced striking defects in the proliferation, migration, and settling of the Purkinje cells; reduced the size of the cerebellar cortex and nuclei; produced defects in the patterning of foliation; and also affected litter size, body weight, and mortality of the offspring. The observed toxic effects were consistent within individual treatment groups but varied between different treatment groups. Treatment with BrdU at the peak of neurogenesis of cerebellar projection neurons (E14) produced the most severe malformations. We observed no overt effects on the timing of neurogenesis for cerebellar neurons and glia across experimental groups. In conclusion, BrdU is a useful tool to study neural development, but its cytotoxicity represents a serious pitfall particularly when multiple doses are used to label cells.

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Section: Cerebellar Patterning Is Affected By Exposure To Brdusupporting

confidence: 87%

Section: Cerebellar Patterning Is Affected By Exposure To Brdumentioning

confidence: 99%

Bromodeoxyuridine administered during neurogenesis of the projection neurons causes cerebellar defects in rat

Sekerková

Ilijić

Mugnaini

2004

J of Comparative Neurology

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“…Prenatal irradiation at 1.0 Gy on E14 led to severe defects of neuronal migration and laminar formation, and in the formation of cortical afferent and efferent pathways in the cerebral cortex in rats (Li et al, 2005). From E14 to E15, it was also the vulnerable period of the Purkinje cells to X-irradiation, as the progenitors of Purkinje cells are under active division (Li et al, 2002). Prenatal protracted gamma-irradiation delivered from E13 to E16 at a total dose of 3 Gy induces severe neuronal loss in rat hippocampus and cerebellum (Schmitz et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of prenatal irradiation with accelerated heavy-ion beams on postnatal development in rats: III. Testicular development and breeding activity

Wang¹,

Murakami²,

Eguchi-Kasai³

et al. 2007

Advances in Space Research

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“…It has been documented that neurongenesis of the cerebellar cortex was fairly complete in the newborn chick and horse, but not in man or rodent (Sisken et al, 1993). In the rat, although Purkinje cell differentiation and migration is between E16 and E20 days (Li et al, 2002), which is similar to the fetal development of major cell types in the cerebellum of the monkey (Makori et al, 2001) and that of the early postnatal pig (present study), the expansion of the dendritic trees, in particular that of the Purkinje cells developed later postnatally in the rat (Chang et al, 1981) and in the pig in this study. Recordable functional activity actually comes at a much later stage in the cerebellum, around 6 months as revealed by prominent fMRI for sensory activities.…”

Section: Discussionmentioning

confidence: 99%

The Postnatal Development of the Cerebellum— A fMRI and Silver Study

Fang

Zhang

et al. 2005

Cell Mol Neurobiol

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The aim of this study was to evaluate the postnatal development of the cerebella of the pig and to compare this with the activation of the fMRI. The cells in the cerebella were studied by silver technique and the activation of the fMRI in the cerebella was initiated by flexion and extension of the hind paw. Our results showed an increase of the branching of the cells of the cerebellar cortex postnatally, coordinated with registration of fMRI active sites in the cerebella at 6-month postnatal. We concluded that the full maturation of the cerebella was around 6-month postnatal in the pig.

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The effect of the timing of prenatal X-irradiation on Purkinje cell numbers in rat cerebellum

Cited by 23 publications

References 37 publications

Bromodeoxyuridine administered during neurogenesis of the projection neurons causes cerebellar defects in rat

Bromodeoxyuridine administered during neurogenesis of the projection neurons causes cerebellar defects in rat

Effects of prenatal irradiation with accelerated heavy-ion beams on postnatal development in rats: III. Testicular development and breeding activity

The Postnatal Development of the Cerebellum— A fMRI and Silver Study

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