The effect of the removal of the various accessory sex glands on the fertility of male rats

Queen, Kennedy L.; Dhabuwala, C.B.; Pierrepoint, C. G.

doi:10.1530/jrf.0.0620423

Cited by 100 publications

(64 citation statements)

References 8 publications

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“…The process of insemination is no longer viewed simply as the delivery of sperm into the female reproductive tract [1]. Although this might be the primary purpose of insemination, there is now evidence that semen can act directly on tissues in the female reproductive tract to elicit accessory functions which in turn may influence implantation, development of the pre-implantation embryo and future health of the offspring.…”

Section: Introductionmentioning

confidence: 99%

Seminal fluid and reproduction: much more than previously thought

Bromfield

2014

J Assist Reprod Genet

123

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The influence of seminal plasma on the cytokine and immune uterine environment is well characterised in mice and humans, while the effects of disruption to uterine seminal plasma exposure on pregnancy and offspring health is becoming more clearly understood. The cellular and molecular environment of the uterus during the pre-and peri-implantation period of early pregnancy is critical for implantation success and optimal foetal and placental development. Perturbations to this environment not only have consequences for the success of pregnancy and neonatal health and viability, but can also drive adverse health outcomes in the offspring after birth, particularly the development of metabolic disorders such as obesity, hypertension and insulin resistance. It is now reported that an absence of seminal plasma at conception in mice promotes increased fat accumulation, altered metabolism and hypertension in offspring. The evidence reviewed here demonstrates that seminal plasma is not simply a transport medium for sperm, but acts also as a key regulator of the female tract environment providing optimal support for the developing embryo and benefiting future health of offspring.

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Section: Introductionmentioning

confidence: 99%

Seminal fluid and reproduction: much more than previously thought

Bromfield

2014

J Assist Reprod Genet

123

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“…However, contradictory results about the effect of this binding have been shown, such as inducing capacitation of rat sperm (Manco and Abrescia, 1988;Metafora et al, 1988;Peitz 1988) and suppressing capacitation of mouse sperm (Huang et al, 2000). Furthermore, the removal of the seminal vesicles of several species induced complete infertility (Davis and Niwa, 1974;Queen et al, 1981;Peitz and Olds-Clarke, 1986), greatly decreased the pregnancy rate and litter size of mouse (Pang et al, 1979), and induced a decreased motility of uterine sperm and pregnancy rate (Peitz and Olds-Clarke, 1986). These results indicate the important role of SVS in mammalian sperm function.…”

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confidence: 99%

Immunohistochemical Localization of Sperm‐Preserving Proteins in the Ram Reproductive Tract

Fernández‐Juan

Gallego

Barrios

et al. 2006

Journal of Andrology

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Previously, we reported that the addition of seminal plasma proteins before cold-shock treatment prevents sperm membrane injury, and that 2 proteins of approximately 14 (P14) and 20 (P20) kDa, the main components of fraction 6 isolated by exclusion chromatography, are responsible for this protective effect. The objective of the present study was to localize P14 and P20 in tissues of the ram reproductive tract to determine their origin. Antiserum generated against purified P14 and P20 reacted with proteins in seminal vesicles and vas deferens by Western blot analyses of protein tissue extracts. However, these antisera failed to detect P14 and P20 in testis, prostate, efferent ductules, bulbourethral glands, and epididymis (caput, corpus, and cauda). Immunohistochemical analyses by both indirect immunofluorescence and the avidin-biotin complex technique confirmed that only seminal vesicles showed reactivity, restricted to the secretory cells, with both antibodies. Obtained results indicate that P14 and P20 are secreted specifically in the seminal vesicles. To further confirm that P14 and P20 are specifically expressed in seminal vesicles, we used Northern blot analyses to investigate the expression of both proteins in seminal vesicles and vas deferens. These assays corroborated again that P14 and P20 were specifically expressed in seminal vesicles. Consequently, we suggest referring to these 2 proteins as RSVP14 and RSVP20, respectively, according to their origin and molecular weight.

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“…Little is known about the role of SVS in male reproduction, but it has been reported that the removal of the seminal vesicles leads to reduced fertility (Pang et al, 1979;Queen et al, 1981 ;Peitz and Olds-Clarke, 1986). As an ongoing effort to understand the role of Feminal plasma components in reproductive biology, we describe here some molecular properties of the major proteins in mouse seminal vesicular fluid.…”

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confidence: 99%

Chemical Characterization of the Predominant Proteins Secreted by Mouse Seminal Vesicles

Lundwall¹,

Peter²,

Lövgren³

et al. 1997

European Journal of Biochemistry

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Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein to human semenogelins, despite the vast difference in primary structure. As previously reported for other seminal-vesicle-secreted proteins, the semenoclotin transcripts are shown to be under androgen control.Keywords: seminal vesicle ; semen ; androgen; transglutaminase ; coagulation.Mammalian semen is formed by the mixing of spermatozoarich fluid from epididymis with secretions from the accessory sex glands during ejaculation, that mainly consists of fluids from the seminal vesicles and the prostate (Mann and Lutwark-Mann, 1981). In most species, the major contribution is provided by the seminal vesicles, amounting to more than half of the semen volume. The newly ejaculated sperm is highly viscous or gellike, but within a short time it will either liquefy, as in man, or transform into a firm coagulum, yielding a copulatory plug through the action of a prostate-secreted transglutaminase, as seen in rodents.The proteins responsible for gel and clot formation are present at very high concentrations in the seminal vesicle fluid. Somewhat unexpectedly, the primary structure of these proteins are highly dissimilar between species (Lundwall and Lazure, 1995; Lundwall and Ulvsback, 1996). This is caused by a relatively recent evolution of an ancestral gene, involving duplications, exon expansion, and varying selection of splice sites. The genes constitute the REST gene family (abbreviation for rapidly evolving substrates for transglutaminase) as most members seem to encode substrates for transglutaminase (Lundwall, 1996 : Lundwall and Ulvsback, 1996).The rat seminal vesicle secretion consists of five predominant protein components, designated seminal-vesicle-secreted proteins I-V (SVS I-V) in order of size (Ostrowski et al., 1979). The SVS IV and V components are also referred to as S and F proteins by some authors (Higgins et al., 1976). The strucCorrespondence to A. Lundwall,

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The effect of the removal of the various accessory sex glands on the fertility of male rats

Abstract: Summary. Accessory sex glands were removed from rats which were then allowed to mate with females.

Cited by 100 publications

References 8 publications

Seminal fluid and reproduction: much more than previously thought

Seminal fluid and reproduction: much more than previously thought

Immunohistochemical Localization of Sperm‐Preserving Proteins in the Ram Reproductive Tract

Chemical Characterization of the Predominant Proteins Secreted by Mouse Seminal Vesicles

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