The effect of the promoter on expression of VP60 gene from rabbit hemorrhagic disease virus in potato plants

Castañón, Sonia; Martı́n-Alonso, José M.; Marín, María Soledad Catalán; Boga, José Antonio; Alonso, P. Vega; Parra, Francisco; Ordás, Ricardo J.

doi:10.1016/s0168-9452(01)00535-0

Cited by 35 publications

(34 citation statements)

References 41 publications

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“…Measured PAT activity was higher on average among the lines that had undergone cryopreservation than in the non-cryopreserved ones (data not shown). This increase could be due to the stress caused by the treatment, results that are in agreement with previous reports on the stress-inducible ubiquitin promoter, which boosted high levels of GUS activity in dicotyledonous (Binet et al 1991;Castañón et al 2002) and monocotyledonous species (Christensen and Quail 1996), and also in conifers (Humara et al 1999a, b;López et al 2000). These data suggest that genetic transformation did not affect the success of the cryopreservation procedure and this did not affect the stability of the transgenic lines.…”

Section: Resultssupporting

confidence: 91%

Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance

Álvarez

Humara³

et al. 2009

Biotechnol Lett

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The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.

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Section: Resultssupporting

confidence: 91%

Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance

Álvarez

Humara³

et al. 2009

Biotechnol Lett

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“…Using the expression vector pNEKhPL1, the expression levels reached a maximum of 0.21% of TSP. These expression values are similar to those reported by other authors using vectors similar to pNEKhPL1 (Castañón et al, 2002;Kim et al, 2003;Mason et al, 1998;Ritcher et al, 2000). Compared to the levels obtained in tobacco plants (Urreta et al 2010), those reached in potato plants are much lower.…”

Section: Case Study: Production Of Human Placental Lactogen In Transgsupporting

confidence: 90%

“…These results are in the range of expression levels reported by other Authors in potato tubers using CaMV35S promoter (Mason et al, 1998;Zhou et al, 2003;Bielmet et al, 2003). Although CaMV35S promoter is not tuber specific, the expression levels obtained are also similar to those reported by Mason et al (1996) and Castañón et al (2002) using tuber specific promoters such as patatin. It is also noteworthy that from the tubers tested, 4 of them did not show detectable levels of hPL.…”

Section: Case Study: Production Of Human Placental Lactogen In Transgsupporting

confidence: 83%

Transgenic Plants as Biofactories for the Production of Biopharmaceuticals: A Case Study of Human Placental Lactogen

Urreta¹,

Castañón²

2012

Transgenic Plants - Advances and Limitations

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“…Therefore, the lower protein levels detected in plants grown in vitro could be due to the stressful conditions of culture. Other authors also reported this changed behavior in protein expression levels between plants grown in vitro and in the greenhouse (Castañón et al 2002).…”

Section: Discussionmentioning

confidence: 68%

Tobacco as biofactory for biologically active hPL production: a human hormone with potential applications in type-1 diabetes

Urreta

Oyanguren²,

Castañón

2010

Transgenic Res

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Human placental lactogen (hPL) is a peptidic hormone that belongs to the short list of growth factors that could treat type-1 diabetes through pancreatic islet transplantation. Placental lactogen has the capacity to improve islet survival and function before or after transplantation. In this study, transgenic tobacco plants were used as a novel expression system for the production of recombinant hPL protein (rhPL). The expression vector pNEKhPL2 containing hPL cDNA was introduced into tobacco plants; the transcriptional activity was confirmed by real-time PCR, and the rhPL levels reached 1% of the total soluble protein (TSP) content in plants cultivated in the greenhouse. In vitro bioassays using the rat insulinoma (INS-1) cell line showed that recombinant protein was able to induce cell proliferation and activate the JAK-2/STAT-5 signal transduction pathway, demonstrating that plant cells can produce the biologically active hPL protein. To further characterize the plant expression system for hPL production, we analyzed the stability of the protein during the life cycle of tobacco plants as well as the transmission of the transgenic trait to the progeny. The recombinant protein was stably accumulated in young leaves, reaching the maximum level in the first month (6.51 μg/g of fresh weight), but showing a decreasing trend of 26% from the initial sampling time until the end of plant's life cycle. The progeny of the selected pNEKhPL2 plant showed in vitro expression levels of up to 1.1% of TSP. Our results therefore indicate that transgenic plants are a suitable expression system for hPL production.

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The effect of the promoter on expression of VP60 gene from rabbit hemorrhagic disease virus in potato plants

Cited by 35 publications

References 41 publications

Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance

Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance

Transgenic Plants as Biofactories for the Production of Biopharmaceuticals: A Case Study of Human Placental Lactogen

Tobacco as biofactory for biologically active hPL production: a human hormone with potential applications in type-1 diabetes

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