The effect of the local delivery of alendronate on human adipose-derived stem cell-based bone regeneration

Wang, Chau-Zen; Chen, Shih-Mao; Ch, Chen; Wang, Chih-Kuang; Wang, Gwo-Jaw; Chang, Je-Ken; Ho, Mei-Ling

doi:10.1016/j.biomaterials.2010.07.096

Cited by 117 publications

(97 citation statements)

References 35 publications

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“…Previous studies revealed that bisphosphonates can exert a significant apoptosis inductive function on GCT stromal cells with a dose depentdent manner , While they also inhibit adipogenic differentiation in bone marrow stromal cells via ERK and JNK activation (Fu et al, 2008) and stimulate osteogenesis and bone regeneration of human adipose-derived stem cells (hADSC) at a low concentration about 1-10µM (Wang et al, 2010). These results demonstrated bisphosphonates a potent tumor cytotoxic and osteo-inductive agent at different concentration.…”

Section: Discussionmentioning

confidence: 80%

Stimulation of Osteogenic Differentiation in Stromal Cells of Giant Cell Tumour of Bone by Zoledronic Acid

Yang¹,

Zheng²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Therapeutic effects of zoledronic acid (ZOL) on giant cell tumour of bone (GCT) have been proven. Apoptosis induction was considered to be one of the mechanisms of ZOL tumour inhibition. In this study, we presented the possibility of an osteogenic differentiation stimulation mechanism of ZOL and further investigated dosage and time effects. We treated stromal cells of GCT (GCTSC) with ZOL for 48 hours at different concentrations (0 μM, 0.01 μM, 0.1 μM, 1 μM, 5 μM, 30 μM) and assessed apoptotic and osteogenic differentiation markers with immunohistochemical techniques and real-time quantitative RT-PCR. Our results suggested that ZOL enhanced mRNA expression of Cbfa-1, osterix and osteocalcin genes with a maximum effect at 1μM in GCTSC. Time course experiments indicated a time dependent osteogenic differentiation effect. In conclusion, ZOL may be considered as an adjuvant in the treatment of GCT not only by inducing apoptosis but also by stimulating osteogenic differentiation of remaining tumor stromal cells after surgery.

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Section: Discussionmentioning

confidence: 80%

Stimulation of Osteogenic Differentiation in Stromal Cells of Giant Cell Tumour of Bone by Zoledronic Acid

Yang¹,

Zheng²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…31 The bone graft substitute constructs were implanted under anesthesia by intraperitoneal injection of ketamine (Parke-Davis; 10 mg/100 g body weight) in combination with xylazine hydrochloride (Bayer HealthCare; 12 mg/100 g body weight). The hair over the calvarium was shaved with a depilator and cleaned.…”

Section: Critical-sized Calvarial Defects In Ratsmentioning

confidence: 99%

Combination of calcium sulfate and simvastatin-controlled release microspheres enhances bone repair in critical-sized rat calvarial bone defects

Fu¹,

Wang²,

Ch³

et al. 2015

IJN

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Most allogenic bone graft substitutes have only osteoconductive properties. Developing new strategies to improve the osteoinductive activity of bone graft substitutes is both critical and practical for clinical application. Previously, we developed novel simvastatin-encapsulating poly(lactic-co-glycolic acid) microspheres (SIM/PLGA) that slowly release simvastatin and enhance fracture healing. In this study, we combined SIM/PLGA with a rapidly absorbable calcium sulfate (CS) bone substitute and studied the effect on bone healing in critical-sized calvarial bone defects in a rat model. The cytotoxicity and cytocompatibility of this combination was tested in vitro using lactate dehydrogenase leakage and a cell attachment assay, respectively. Combination treatment with SIM/PLGA and the CS bone substitute had no cytotoxic effect on bone marrow stem cells. Compared with the control, cell adhesion was substantially enhanced following combination treatment with SIM/PLGA and the CS bone substitute. In vivo, implantation of the combination bone substitute promoted healing of critical-sized calvarial bone defects in rats; furthermore, production of bone morphogenetic protein-2 and neovascularization were enhanced in the area of the defect. In summary, the combination of SIM/PLGA and a CS bone substitute has osteoconductive and osteoinductive properties, indicating that it could be used for regeneration of bone in the clinical setting.

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“…Studies have shown that locally delivered alendronate has an effect on increased bone repair in a critical-sized calvarial defect (19) and peri-implant bone formation (20) and associated with bone allografts (21). Moreover, alendronate enhances alkaline phosphatase (ALP) activity and bone neoformation (19).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Local Delivery Doxycycline and Alendronate on Bone Repair

et al. 2015

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Abstract. The aim of the present study was to investigate the local effect of 10% doxycycline and 1% alendronate combined with poly(lactic-co-glycolic acid) (PLGA) on bone repair. Thirty rats were divided into three groups, as follows: control group (CG), drug group (DG), and vehicle-PLGA group (VG). Bone defect was created in the right femur and filled with the following: blood clot (CG); PLGA gel, 10% doxycycline and 1% alendronate (DG); or vehicle-PLGA (VG). The animals were euthanized 7 or 15 days after surgery. Bone density, bone matrix and number of osteoclasts were quantified. At 7 days, the findings showed increased density in DG (177.75±76.5) compared with CG (80.37±27.4), but no difference compared with VG (147.1±41.5); no statistical difference in bone neoformation CG (25.6±4.8), VG (27.8±4), and DG (18.9±7.8); and decrease osteoclasts in DG (4.6±1.9) compared with CG (26.7±7.4) and VG (17.3±2.7). At 15 days, DG (405.1±63.1) presented higher density than CG (213.2±60.9) and VG (283.4±85.8); there was a significant increase in percentage of bone neoformation in DG (31.5±4.2) compared with CG (23 ±4), but no difference compared with VG (25.1±2.9). There was a decreased number of osteoclasts in DG (20.7±4.7) and VG (29.5±5.4) compared with CG (40±9.4). The results suggest that the association of 10% doxycycline and 1% alendronate with PLGA-accelerated bone repair.

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The effect of the local delivery of alendronate on human adipose-derived stem cell-based bone regeneration

Cited by 117 publications

References 35 publications

Stimulation of Osteogenic Differentiation in Stromal Cells of Giant Cell Tumour of Bone by Zoledronic Acid

Stimulation of Osteogenic Differentiation in Stromal Cells of Giant Cell Tumour of Bone by Zoledronic Acid

Combination of calcium sulfate and simvastatin-controlled release microspheres enhances bone repair in critical-sized rat calvarial bone defects

The Effect of Local Delivery Doxycycline and Alendronate on Bone Repair

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