The effect of the Knox‐Parcher ratio in microcolumn LC

Wilson, William; McNair, Harold M.; Maa, Yih-Fen; Hyver, Karen J.

doi:10.1002/jhrc.1240130105

Cited by 8 publications

(2 citation statements)

References 5 publications

(1 reference statement)

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“…Several research groups [63,65,94,95] reported that the plate height of packed columns reduces by a factor of approximately 2 when the column to particle diameter ratio -also known as the Knox-Parcher ratiois smaller than 6. Classical plate height equations suggest, however, that the column diameter does not influence the plate height of packed columns -as indicated by the following simplified Horvath-Lin equation:…”

Section: Column Developmentsmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Column Developmentsmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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show abstract

“…There are at least three types of ionizing groups: isolated, vicinal and geminal silanols. Interspersed among these ionogenic groups, one can envision inert siloxane bridges and highly acidic hydrogen bonding sites [2,3]. The total density of ionogenic silanols on this surface is given as 8.31 X M/mz, corresponding to ca.…”

Section: Introductionmentioning

confidence: 99%

Probing the inner surface of a capillary with the atomic force microscope

Barberi¹,

Giocondo²,

Bartolino³

et al. 1995

Electrophoresis

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The inner surface of a fused silica capillary for zone electrophoresis was probed by the atomic force microscope (AFM) in the contact mode. Only uncoated surfaces were analyzed, after a simple washing cycle with detergent, NaOH and HCl, and final equilibration in distilled water. Three progressively larger surface areas were probed: 0.5 x 0.5 micron, 2 x 2 microns and 4 x 4 microns. In all cases, it was found that the surface is remarkably smooth, with a median height increasing from 1.3 to 5.6 nm; mean height, from 1.3 to 5.8 nm; root-mean-squared roughness, from 0.35 to 1.5 nm; and average roughness, from 0.28 to 0.67 nm (the lower values referring to the smaller area, the larger to the largest area probed). The lowest "peaks" detected are of the order of 1-2 nm; some scattered peaks as high as 16 nm are occasionally found. It is concluded that the inner surface of a capillary is not a serrate or notchy structure, but is indeed quite smooth. Since the average roughness is comprised within the thickness of the diffuse double layer (> 10 nm) existing on the silica wall as a result of silanol ionization (and, in fact, it is on the average considerably smaller), it is concluded that it cannot possibly influence peak shape and contribute to peak decay in an electrophoretic run.

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Low aspect ratio packed capillary columns in supercritical fluid chromatography

Pyo

Wan

et al. 1996

J. Micro. Sep.

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The effect of the Knox‐Parcher ratio in microcolumn LC

Cited by 8 publications

References 5 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Probing the inner surface of a capillary with the atomic force microscope

Low aspect ratio packed capillary columns in supercritical fluid chromatography

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