Low aspect ratio packed capillary columns in supercritical fluid chromatography

Li, Wenbao; Pyo, Dongjin; Wan, Yanjian; Ibáñez, Elena; Malik, Abdul; Lee, Milton L.

doi:10.1002/(sici)1520-667x(1996)8:4<259::aid-mcs3>3.0.co;2-0

Cited by 13 publications

(3 citation statements)

References 25 publications

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“…Capillary packings at low column-to-particle diameter (aspect) ratio have been frequently studied for use with liquid chromatography, − supercritical fluid chromatography, and electrochromatography, , and they also played a key role in early UHPLC research. − These studies cover a wide range of packing protocols, particle properties, and applications. It is not surprising that the relation between a packing protocol, the resulting bed morphology and macroscopic separation efficiency, as well as the general impact of the aspect ratio are still insufficiently understood; the individual packing process plays a more important role than a purely geometrical parameter represented by the aspect ratio.…”

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“…We focus on a single parameter in the slurry packing and bed consolidation process, the column inner diameter (varied from d c = 10 to 75 μm) and its impact on the resulting bed morphology. Capillary packings at low column-to-particle diameter (aspect) ratio have been frequently studied for use with liquid chromatography, 29−32 supercritical fluid chromatography, 33 and electrochromatography, 34,35 and they also played a key role in early UHPLC research. 36−39 These studies cover a wide range of packing protocols, particle properties, and applications.…”

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Morphology and Separation Efficiency of Low-Aspect-Ratio Capillary Ultrahigh Pressure Liquid Chromatography Columns

et al. 2012

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We derive a quantitative relationship between the bed morphology and the chromatographic separation efficiency of capillary columns packed with sub-2 μm particles, covering capillary inner diameters from 10 to 75 μm. Our study focuses on wall effects and their impact on band broadening at increasing column-to-particle diameter (aspect) ratios. We approach these complex effects by a morphological analysis of reconstructed column segments composed of several thousand particles that were imaged by confocal laser scanning microscopy. Radial interparticle porosity profiles including wall effects are quantified through an integral porosity deviation, a scalar measure that proves to be a general descriptor of transcolumn porosity heterogeneity. It correlates with the associated transcolumn eddy dispersion, which dominates band broadening in the capillaries and is visualized in the plate height curves by a simple velocity-proportional term. Our comprehensive approach identifies the packing structure features that contribute to decreased efficiency as reflected, e.g., in subtle variations of the wall effect at different aspect ratios, or a particle size-segregation effect in larger-diameter columns as a result of an increased number of packing voids near the wall-bed interface.

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Morphology and Separation Efficiency of Low-Aspect-Ratio Capillary Ultrahigh Pressure Liquid Chromatography Columns

et al. 2012

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“…Zhang et al reported an in situ deactivation of packings and characterized them using NMR (61). Li et al explored the use of packed-capillary columns with low column diameter-to-particle diameter ratios (62).…”

Section: Supercritical Fluid Chromatographymentioning

confidence: 99%

Supercritical Fluid Chromatography and Extraction

1998

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Development of packed capillary columns using carbon dioxide slurries

Planeta¹,

Karásek²,

Vejrosta³

2003

J of Separation Science

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Development of packed capillary columns using carbon dioxide slurriesA modified procedure for the preparation of packed capillary columns by the use of supercritical or liquid carbon dioxide slurries is proposed. This technique removes the problems arising from sudden and rapid movement of sorbent suspension at the beginning of the filling process. The parameters influencing the packing process (temperature, pressure, and restrictor that controls the flow rate of CO 2 ), have been optimized. The columns prepared under optimized conditions exhibit high efficiency (average value of the reduced plate height h 9 ca. 2.2), and long term stability. An example of the SFC separation of polydimethylsiloxanes in silicone oil is presented.

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Low aspect ratio packed capillary columns in supercritical fluid chromatography

Cited by 13 publications

References 25 publications

Morphology and Separation Efficiency of Low-Aspect-Ratio Capillary Ultrahigh Pressure Liquid Chromatography Columns

Morphology and Separation Efficiency of Low-Aspect-Ratio Capillary Ultrahigh Pressure Liquid Chromatography Columns

Supercritical Fluid Chromatography and Extraction

Development of packed capillary columns using carbon dioxide slurries

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