The effect of the deuterium depleted water on the biological activity of the eukaryotic cells

Сыроешкин, А. В.; Антипова, Н. В.; Zlatska, Alona; Zlatskiy, I. A.; Skylska, M.D.; Гребенникова, Т. В.; Гончарук, В. В.

doi:10.1016/j.jtemb.2018.05.004

Cited by 30 publications

(36 citation statements)

References 22 publications

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“…Our findings may indicate a reduction in systemic inflammation in obese animals treated with DDW. Perhaps this result is due to the ability of deuterium to slow down or speed up the reaction rate of intracellular processes include decreased systemic oxidative stress resulting in restoration of the normal functioning of the liver, adipose and muscle tissues and/or an influence of DDW on the balance between the production of pro-and anti-inflammatory cytokines [29,30]. DDW has previously been shown to have chirality capabilities mediated, partially or completely by alterations in metabolic pathways in live systems [18].…”

Section: Discussionmentioning

confidence: 99%

“…The deuterium content in the water was measured by multipass laser absorption spectroscopy on an Isotopic Water Analyzer-912-0032 (Los Gatos Research, Inc., San Jose, CA, USA). Detailed description of the method was presented in previous studies [29,30].…”

Section: Physicochemical Analysis Of Water With Different Deuterium Rmentioning

confidence: 99%

“…Chemical analysis of water with different deuterium ratios was performed by inductively coupled plasma-mass spectrometry on an ICP-QMS Agilent 7500CE spectrometer (Agilent Technologies, Santa Clara, CA, USA). Detailed description of the methods was presented in previous studies [29,30]. Calibration solutions with a high range of element concentrations (from 0.1 µg·L −1 to 100 µg·L −1 ) were used to calibrate the device.…”

Section: Physicochemical Analysis Of Water With Different Deuterium Rmentioning

confidence: 99%

“…Limited data have shown that deuterium in aqueous solutions can stimulate or inhibit metabolic processes in living systems [23][24][25][26][27]. As mentioned above, DDW has a number of unexpected biological properties, including antitumor [28], antidotal [29] and metabolic effects [30]. However, whether deuterium is involved in the regulation of biological properties of in-vivo models has not been fully characterized [26,27].…”

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confidence: 99%

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Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

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In this study, we present the potential application of deuterium-depleted water (DDW) for the prevention and adjuvant treatment of obesity in rats. We tested the hypothesis that DDW can alleviate diet-induced obesity (DIO) and its associated metabolic impairments. Rats fed a high-fat diet had an increased body weight index (BWI), glucose concentration, and level of certain proinflammatory cytokines; decreased levels of insulin in the serum; decreased tryptophan and serotonin in the brain, and a decreased concentration of some heavy metals in the liver. Drinking DDW at a concentration of 10 ppm deuterium/protium (D/H) ad libitum for 3 weeks restored the BWI, glucose (serum), tryptophan (brain), and serotonin (brain) levels and concentration of Zn in the liver in the DIO animals to those of the controls. The levels of proinflammatory cytokines (IL-1β, IL-6, IFNγ) and anti-inflammatory TNFα were decreased in DIO rats, while anti-inflammatory cytokine (IL-4, IL-10) levels remained at the control levels, which is indicative of a pathophysiological syndrome. In contrast, in groups of rats treated with DDW, a significant increase in anti-inflammatory (IL-4, IL-10) and proinflammatory cytokines (IFNγ) was observed. This finding indicates a reduction in systemic inflammation in obese animals treated with DDW. Similarly, the high-fat diet caused an increased level of oxidative stress products, which was accompanied by decreased activity of both superoxide dismutase and catalase, whereas the administration of DDW decreased the level of oxidative stress and enhanced antioxidant enzyme activities.Molecules 2020, 25, 23 2 of 15 the development of new, safer and more effective pharmaceutical approaches for obesity treatment is essential.Natural water is a mixture of molecules containing stable isotopes 16 O, 17 O, 18 O, 1 H, and 2 H. The ratio of deuterium atoms to protium (D/H) in fresh and marine water is 132-156 µg/g (ppm) [7,8].Since the mass of protium H is half of the mass of deuterium D, effects arising from the replacement of isotopes influence biological systems; for example, the rates of chemical reactions between substances containing these isotopes may differ by a factor of 5-10 [9-11].Numerous publications over the last 20 years have been devoted to the study of the role of deuterium in natural (150 ppm) or reduced content in the human body [12]. It has been shown that deuterium-depleted water (DDW) exhibits antidotal properties with individual and combined effects on the dosage of pharmaceutical substances and auxiliary substances [13][14][15][16]. The mechanisms of such influence are due to the structure, physicochemical properties of DDW [17] and changes in ligand-receptor interactions in biological objects of different hierarchical levels [18]. DDW increases the rate of photosynthesis and promotes the growth of plants and aquatic animals [19,20]. In Europe, the USA, Japan, China and Russia, DDW is used for prophylactic and therapeutic purposes [21,22]. Currently, DDW is using as an adjuvant in cancer treatment ...

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Section: Discussionmentioning

confidence: 99%

Section: Physicochemical Analysis Of Water With Different Deuterium Rmentioning

confidence: 99%

Section: Physicochemical Analysis Of Water With Different Deuterium Rmentioning

confidence: 99%

mentioning

confidence: 99%

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Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

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“…e concentration of all above-listed 24 elements in the Milli-Q, deuterium-depleted, or heavy water did not exceed the upper detection limit (detection limit range: 0.1-10 ppm). Detailed description of the methods was presented in previous studies [14,22].…”

Section: Physicochemical Analysis Of Water With Different Deuterium Cmentioning

confidence: 99%

Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Zlatskiy

Zlatska

Антипова

et al. 2020

The Scientific World Journal

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In this study, using new approach (laser diffraction + biological dyes), we have demonstrated the decrease of cells viability in vitro in the deuterated growth medium, whereas in the deuterium-depleted medium, there was an increase of cell viability. We have also found that not all dyes are equally sensitive to the D/H ratios in the culture medium (system) as well as to the different cell types (cancer vs normal cells).

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Deuterium‐depleted water: A new tracer to label pulmonary surfactant lipids in adult rabbits

et al. 2022

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Stable isotope tracing can be safely used for metabolic studies in animals and humans. The endogenous biosynthesis of lipids (lipogenesis) is a key process throughout the entire life but especially during brain and lung growth. Adequate synthesis of pulmonary surfactant lipids is indispensable for life. With this study, we report the use of deuterium-depleted water (DDW), suitable for human consumption, as metabolic precursor for lipogenesis. We studied 13 adult rabbits for 5 days. Four rabbits drank tap water (TW) and served as controls; in four animals, DDW was substituted to drinking water, whereas five drank deuterium-enriched water (DEW). After 5 days, a blood sample and a bronchoalveolar lavage (BAL) sample were collected. The 2 H/ 1 H (δ 2 H) of BAL palmitic acid (PA) desaturated phosphatidylcholine (DSPC), the major phospholipid of pulmonary surfactant, and of plasma water was determined by high-resolution mass spectrometry. We found that the δ 2 H values of DDW, DEW and TW were À984 ± 2‰, +757 ± 2‰ and À58 ± 1‰, respectively. After 5 days, plasma water values were À467 ± 87‰, +377 ± 56‰ and À53 ± 6‰, and BAL DSPC-PA was À401 ± 27‰, À96 ± 38‰ and À249 ± 9‰ in the DDW, DEW and TW, respectively. With this preliminary study, we demonstrated the feasibility of using DDW to label pulmonary surfactant lipids. This novel approach can be used in animals and in humans, and we speculate that it could be associated with more favourable study compliance than DEW in human studies.

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The effect of the deuterium depleted water on the biological activity of the eukaryotic cells

Cited by 30 publications

References 22 publications

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Deuterium‐depleted water: A new tracer to label pulmonary surfactant lipids in adult rabbits

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