Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Zlatskiy, I. A.; Zlatska, Alona; Антипова, Н. В.; Доленко, С. А.; Gordiienko, I M; Gubar, Olga; Vasyliev, Roman; Zubov, D. A.; Новикова, С. Н.; Сыроешкин, А. В.

doi:10.1155/2020/2373021

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…The benzotriazolyl esters were tested in terms of cytotoxic activities in A375 human melanoma cells and HaCaT healthy keratinocytes by employing the Alamar Blue cell viability test; cell viability translates into the cells’ ability to perform a specific function or to engage in the mitotic process. The assessment of cell viability is necessary either to establish cell morphological integrity or to monitor cell reactions under various stimuli including toxins or other pharmacologically active compounds [ 22 ]. In the current study, all three semisynthetic compounds exerted a significant dose-dependent cytotoxic effect in melanoma cells when used in concentrations above 25 μΜ; their biological activities exceeded in all cases the ones recorded for the parent compounds.…”

Section: Discussionmentioning

confidence: 99%

Novel Triterpenic Acid—Benzotriazole Esters Act as Pro-Apoptotic Antimelanoma Agents

Mioc

Prodea

et al. 2022

IJMS

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Pentacyclic triterpenes, such as betulinic, ursolic, and oleanolic acids are efficient and selective anticancer agents whose underlying mechanisms of action have been widely investigated. The introduction of N-bearing heterocycles (e.g., triazoles) into the structures of natural compounds (particularly pentacyclic triterpenes) has yielded semisynthetic derivatives with increased antiproliferative potential as opposed to unmodified starting compounds. In this work, we report the synthesis and biological assessment of benzotriazole esters of betulinic acid (BA), oleanolic acid (OA), and ursolic acid (UA) (compounds 1–3). The esters were obtained in moderate yields (28–42%). All three compounds showed dose-dependent reductions in cell viability against A375 melanoma cells and no cytotoxic effects against healthy human keratinocytes. The morphology analysis of treated cells showed characteristic apoptotic changes consisting of nuclear shrinkage, condensation, fragmentation, and cellular membrane disruption. rtPCR analysis reinforced the proapoptotic evidence, showing a reduction in anti-apoptotic Bcl-2 expression and upregulation of the pro-apoptotic Bax. High-resolution respirometry studies showed that all three compounds were able to significantly inhibit mitochondrial function. Molecular docking showed that compounds 1–3 showed an increase in binding affinity against Bcl-2 as opposed to BA, OA, and UA and similar binding patterns compared to known Bcl-2 inhibitors.

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Section: Discussionmentioning

confidence: 99%

Novel Triterpenic Acid—Benzotriazole Esters Act as Pro-Apoptotic Antimelanoma Agents

Mioc

Prodea

et al. 2022

IJMS

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“…The deuterium concentration in the body plays an important role in the metabolic processes of living systems [ 1 , 2 , 3 , 4 ]. The biological effects caused by deuterium depleted water (DDW) have been studied at various levels: cellular [ 5 , 6 , 7 , 8 ], tissue [ 9 ], organismic [ 10 , 11 , 12 , 13 , 14 ]. It was found that low concentrations of deuterium in the drinking diet increase stress resistance in mammals [ 15 ], and may also have neuroprotective properties [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

et al. 2020

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In the present study, the effect of 2H/1H isotopic exchange in hydrogen bonds between nitrogenous base pairs on occurrence and open states zones dynamics is investigated. These processes are studied using mathematical modeling, taking into account the number of open states between base pairs. The calculations of the probability of occurrence of open states in different parts of the gene were done depending on the localization of the deuterium atom. The mathematical modeling study demonstrated significant inequality (dependent on single 2H/1H replacement in DNA) among three parts of the gene similar in length of the frequency of occurrence of the open states. In this paper, the new convenient approach of the analysis of the abnormal frequency of open states in different parts of the gene encoding interferon alpha 17 was presented, which took into account both rising and decreasing of them that allowed to make a prediction of the functional instability of the specific DNA regions. One advantage of the new algorithm is diminishing the number of both false positive and false negative results in data filtered by this approach compared to the pure fractile methods, such as deciles or quartiles.

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“…No response was observed in the A431 cells based on Trypan blue following treatment with both the WS extract and wsCDs (data not shown).This could be due to the lack of Trypan blue® assay sensitivity in comparison to the Alamar blue™ assay. 42 Due to their absorbance in the UVA (315-400 nm) and UVB (280-315 nm) regions, CDs and their composites have gained importance as UV protectors. 18,[43][44][45][46] In comparison to their parent materials (WS extract), wsCDs showed a 3.6× enhanced…”

Section: Biocompatibility Photo-protection and Antioxidant Activity O...mentioning

confidence: 99%

Withania somnifera-derived carbon dots protect human epidermal cells against UVB-induced cell death and support growth factor-mediated wound healing

et al. 2023

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Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

Cited by 9 publications

References 38 publications

Novel Triterpenic Acid—Benzotriazole Esters Act as Pro-Apoptotic Antimelanoma Agents

Novel Triterpenic Acid—Benzotriazole Esters Act as Pro-Apoptotic Antimelanoma Agents

Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA

Withania somnifera-derived carbon dots protect human epidermal cells against UVB-induced cell death and support growth factor-mediated wound healing

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