The effect of the cell-derived extracellular matrix membrane on wound adhesions in rabbit strabismus surgery

Abstract

The cell-derived extracellular matrix (ECM) membrane is a bio-absorbable membrane composed of aseptic ECM obtained from swine knee chondrocyte. The purpose of our study was to evaluate the inhibitory effect of the cell-derived ECM membrane in postoperative adhesion following strabismus surgery on rabbit eyes. Twenty four adult male New Zealand albino rabbits were used. Each rabbit has undergone 3 mm resection of the superior rectus muscle in both eyes. The cell-derived ECM membrane was applied between the conjunctiva and superior rectus muscle only to right eye in each rabbit. The eyes were divided into a surgery-ECM group and a surgery-no ECM group. The rabbits were sacrificed at 2, 4, and 6 weeks after the surgery. Each time, 16 eyes of 8 rabbits were enucleated to observe the adhesion in muscle and adjacent tissue grossly by blunt dissection. Then, histopathological sections were analyzed for inflammation and fibrosis by staining with hematoxylin-eosin and Masson trichrome, respectively. Inflammation and fibrosis were graded on a scale of 0 to 4. A researcher was blind to the experiments performed all the examinations. Comparing with the surgery-no ECM group, there was no significant difference with regard to inflammation at the area of superior rectus muscle resection at 2, 4, and 6 postoperative weeks (p = 0.52, p = 0.55, p = 0.82). However, a significant reduction in the degree of adhesion (p = 0.01, p = 0.03, p = 0.04) as well as decreased fibrosis (p = 0.04, p = 0.03, p = 0.02) between muscle and conjunctiva after strabismus surgery was observed in all surgery-ECM groups. The current results demonstrated that the cell-derived ECM membrane could inhibit the formation of postoperative adhesion and fibrosis between conjunctiva and muscle. The authors concluded that the use of ECM membrane could reduce postoperative adhesion around surgical area after strabismus surgery in rabbits.

Preparation of Extracellular Matrix Developed Using Porcine Articular Cartilage and In Vitro Feasibility Study of Porcine Articular Cartilage as an Anti-Adhesive Film

“…Currently, polysaccharide-based film or gel-type anti-adhesive barriers, such as sodium hyaluronate, gelatin, and carboxymethyl cellulose, have been extensively investigated and are commercially available for clinical utilization [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. In addition, several extracellular matrices, such as collagen, have been investigated in preclinical animal studies as anti-adhesive barriers [ 11 , 12 , 13 ].…”

Efficacy of collagen matrix implant on adhesions in restrictive strabismus: An experimental study in a rabbit model

“…and superior rectus muscle belly in order (Sheha et al 2009). After reattaching the muscle to its previous insertion using 7-0 vicryl sutures, the redundant portion of AM was folded on each side to wrap the muscle (Sheha et al 2009;Kassem et al 2011;Yang et al 2014).…”

Alginate-hyaluronic acid-collagen composite hydrogel favorable for the culture of chondrocytes and their phenotype maintenance

“…Chondrocytes are easily dedifferentiated in in vitro cultures, and it is difficult to obtain enough population of cells required. To address this limitation well-designed culture systems or 3D scaffolds for chondrocytes should be established [7][8][9][10].…”