The Effect of Temperature on the Behavior of Malic Acid and Starch in Leaves of Bryophyllum calycinum Cultured in Darkness.

Vickery, Hubert Bradford

doi:10.1104/pp.29.4.385

Cited by 31 publications

(23 citation statements)

References 7 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The curve for organic solids in figure 1 shows that a small increase occurred during the first 12 hours in darkness as would be expected from previous work (1,5) The samples cultured in light increased slightly in fresh weight for the first 14 hours, but subsequently lost weight continuously and became markedly flaccid; nevertheless, on transfer to darkness, they retained a moderate capacity to take up water again although only the sample exposed to light for 27 hours recovered all of its initial fresh weight.…”

Section: Resultssupporting

confidence: 63%

See 1 more Smart Citation

The Capacity of Leaves of Bryophyllum calycinum to Recover from Prolonged Exposure to Darkness or to Light.

Vickery

1956

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 63%

“…Samples were also removed from the culture troughs in light and dried for analysis at times indicated in the figures. Fuller details of the technique and of the treatment of the samples in preparation for chemical analysis have been given in previous papers (1,5). The analytical methods are described in recent bulletins from this station (6,7).…”

mentioning

confidence: 99%

The Capacity of Leaves of Bryophyllum calycinum to Recover from Prolonged Exposure to Darkness or to Light.

Vickery

1956

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

“…This is well brought out by the data of table IV, and was also emphasized by the results of a study of the effects of temperature upon the organic acid metabolism (18). The rate at which citric acid was formed in the tissues in darkness at 60 C was only a little more than one half that at 24°, although there was little effect upon the rates at which malic acid was synthesized at these 2 temperatures during the 1st 18 hours of the test.…”

Section: Previous Observations On the Effect Of Light On The Mietabolsupporting

confidence: 55%

Effect of Light upon the Behaviour of Citric Acid in Leaves of Bryophyllum Calycinum Salisb.

Vickery

1959

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

“…3,27,28,41). The nightly acidification of the leaves results primarily from an enzymatic carboxylation of PEP with atmospheric CO2 which yields oxalacetate and, through subsequent reduction, malate (37). In mits a closure of the stomata during the hot daytime by making CO, available for photosynthesis from the endogenous acid pools (24).…”

mentioning

confidence: 99%

The Relation between Photosynthesis, Respiration, and Crassulacean Acid Metabolism in Leaf Slices of Aloe arborescens Mill

Denius¹,

Homann²

1972

Plant Physiol.

View full text Add to dashboard Cite

Leaves and leaf slices from Aloe arborescens Mill. were used to study the interrelations between Crassulacean acid metabolism, photosynthesis, and respiration. Oxygen exchange of leaf slices was measured polarographically. It was found that the photosynthetic utilization of stored malic acid resulted in a net evolution of oxygen. This oxygen produetion, and the decrease in acid content of the leaf tissue, were completely inhibited by amytal, although the rate of respiratory oxygen uptake was hardly affected by the presence of this inhibitor of mitochondrial electron transport. Other poisons of respiration (cyanide) and of the tricarboxylic acid cycle (trifluoroacetate, 2-diethyl malonate) also were effective in preventing aciddependent oxygen evolution. It is concluded that the mobilization of stored acids during light-dependent deacidification of the leaves depends on the operation of the tricarboxylic acid cycle and of the electron transport of the mitochondria.A mits a closure of the stomata during the hot daytime by making CO, available for photosynthesis from the endogenous acid pools (24).Many striking resemblances exist between the mechanism of CO. assimilation in CAM and that in the C-dicarboxylic acid pathway of CO, fixation which is characterized by the appearance of labeled dicarboxylic acids as first stable products of the photosynthetic assimilation of 'CO2. Many data and concepts have recently been published on the physiological differences which separate C-plants from plants with the "classical" Calvin-Benson pathway of CO2 fixation (C,-plants). Investigations on CAM, however, have been few. This situation is reflected in Hatch and Slack's (13) review on the mechanisms of CO2 assimilation. The photosynthetic use of CO2 from stored malate in chlorophyllous tissue with CAM should be reflected in a light-dependent net evolution of oxygen even in the absence of external CO,. It was the aim of this work to devise a rapid and reproducible method for the measurement of 02 exchange in such tissue under various conditions. This approach was hoped to permit an analysis of the physiological conditions regulating the storage and photosynthetic utilization of CO2 in plants with CAM.The use of intact leaves was not desirable because their gas exchange is influenced by the activity of the stomata and by the diffusion of gases through the bulky tissue. Sectioning of the leaf would lessen, or eliminate, such problems and facilitate the entry of added inhibitors and other substances. Bruinsma (6) had observed, however, that CAM was adversely affected when Bryophyllum leaves were sliced. This appeared to have been due to actions of leached tannins and other phenolic compounds. Therefore, we had to find a plant which was relatively free of such potentially inhibitory compounds. This criterium eliminated nearly all plants which had been used by other investigators in the past, e.g., from the genera Bryophyllum, Sedum, and Kalanchoe. Leaves from Aloe arborescens Mill. (Liliaceae), however, had a very low conten...

show abstract

The Effect of Temperature on the Behavior of Malic Acid and Starch in Leaves of Bryophyllum calycinum Cultured in Darkness.

Cited by 31 publications

References 7 publications

The Capacity of Leaves of Bryophyllum calycinum to Recover from Prolonged Exposure to Darkness or to Light.

The Capacity of Leaves of Bryophyllum calycinum to Recover from Prolonged Exposure to Darkness or to Light.

Effect of Light upon the Behaviour of Citric Acid in Leaves of Bryophyllum Calycinum Salisb.

The Relation between Photosynthesis, Respiration, and Crassulacean Acid Metabolism in Leaf Slices of Aloe arborescens Mill

Contact Info

Product

Resources

About