The effect of temperature abuse on Clostridium perfringens and Bacillus cereus in raw beef and chicken substrates during frozen storage

White, Cathryn A.; Hall, L.P.

doi:10.1016/0740-0020(84)90018-2

Cited by 4 publications

(1 citation statement)

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“…White and Hall (1984b) stated that enumeration of freeze-damaged mesophiles, such as Salmonella spp., from samples containing mixed flora is difficult. They also found that chicken samples frozen for 6 mo had a 40 to 67% decrease in the number of Clostridium perfringens and a 99% decrease in Bacillus cereus (White and Hall, 1984a). Therefore, it appears that both the freezing process itself and the time of frozen storage damage some cells beyond the point of recovery using standard methods of enumeration.…”

Section: Introductionmentioning

confidence: 89%

Effect of Freezing on the Recovery of Mesophilic Bacteria from Temperature-Abused Broiler Chicken Carcasses

1994

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The effects of freezing on the recovery of mesophilic bacteria from broiler chicken carcasses were determined. Fifty fresh broiler carcasses were used in each of three independent trials. Ten carcasses were sampled at time of collection (Treatment 1: Day 0 control). The other 40 carcasses were sampled at Day 7 following one of four additional treatments with 10 carcasses each: held at 3 C (Treatment 2: 7-d control), frozen and then held at 3 C (Treatment 3: frozen control), temperature abused at 25 C for 12 h and held at 3 C (Treatment 4: temperature abused), and temperature abused at 25 C for 12 h, frozen, and held at 3 C (Treatment 5: temperature abused and frozen). Carcasses were sampled by rinsing, and total plate counts (TPC) expressed as log10 colony-forming units per milliliter and impedance detection times (DT) in hours were determined using incubation temperatures of 42 and 43 C, respectively. Results for TPC for Treatments 1 to 5 were 3.89, 3.52, 2.86, 6.53, and 5.57 log10 cfu/mL, respectively. Results for DT for Treatments 1 to 5 were 5.41, 6.50, 7.10, 2.55, and 3.42 log10 cfu/mL, respectively. Freezing reduced the ability to detect temperature abuse because of a significant reduction in the number of mesophiles recovered using either microbiological technique. Freezing appears to decrease, but may not completely eliminate, the ability of selective incubation to detect temperature abuse.

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Section: Introductionmentioning

confidence: 89%