The effect of TDZ on organogenesis and somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp)

Singh, N.; Sahoo, Lingaraj; Sarin, Neera Bhalla; Jaiwal, Pawan K.

doi:10.1016/s0168-9452(02)00418-1

Cited by 92 publications

(58 citation statements)

References 15 publications

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“…JKR105. Induction of somatic embryogenesis in pigeon pea has been reported on EC6 basal (Patel et al 1994;Mohan and Krishnamurthy 2002) and MS basal media (Nalini et al 1996;Sreenivasu et al 1998;Singh et al 2003) for various Asian and African cultivars of pigeon pea.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…TDZ has been used to elicit multiple shoot formation in a broad range of plant species (Malik and Saxena 1992a,b), and somatic embryogenesis in a few crop species Lu 1993). While other studies indicated successful utilization of cytokinins for somatic embryogenesis not only in pigeon pea (Patel et al 1994;Sreenivasu et al 1998;Mohan and Krishnamurthy 2002;Chandra et al 2003;Singh et al 2003) but also other leguminous crops (Maheswaran and Williams 1984;Gill and Saxena 1992;Malik and Saxena 1992b;Murthy et al 1996). The MS medium comprising TDZ and BAP was found less effective for globular embryo formation with glucose than sucrose.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…Similarly, the somatic embryogenesis has been reported through in vitro cultures from different tissue sources of various pigeon pea cultivars of Asian origin (Nalini et al 1996;Anbazhagan and Ganapathi 1999;Mohan and Krishnamurthy 2002;Chandra et al 2003;Singh et al 2003). Plant regeneration has been achieved via callus (Nalini et al 1996;Sreenivasu et al 1998), suspension cultures from leaf-derived callus (Anbazhagan and Ganapathi 1999), and cotyledonary node (Singh et al 2003).…”

Section: Introductionmentioning

confidence: 96%

“…In pigeon pea, the regeneration of plants by organogenesis has been reported via pre-existing meristems like apical meristem (Cheema and Bawa 1991), undifferentiated callus (Kumar et al 1983;George and Eapen 1994), differentiated non-meristematic leaf tissue Eapen et al 1998;Geetha et al 1998;Singh et al 2002;Dayal et al 2003;Villiers et al 2008); and seedling tissues such as hypocotyl (Cheema and Bawa 1991;Geetha et al 1998), cotyledon (George and Eapen 1994;Geetha et al 1998;Mohan and Krishnamurthy 1998;Chandra et al 2003), cotyledonary node (Mehta and Mohan Ram 1980;Kumar et al 1983Kumar et al , 1984Naidu et al 1995;Shiva Prakash et al 1994;Geetha et al 1998;Singh et al 2003), epicotyl (Kumar et al 1984;Naidu et al 1995;Geetha et al 1998), and embryonal axes (Sarangi and Gleba 1991;George and Eapen 1994;Naidu et al 1995;Franklin et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Plant regeneration has been achieved via callus (Nalini et al 1996;Sreenivasu et al 1998), suspension cultures from leaf-derived callus (Anbazhagan and Ganapathi 1999), and cotyledonary node (Singh et al 2003). Despite several reports available on organogenesis and somatic embryogenesis using cultivars of Asian and African origin, highly reproducible protocols in the same genotype are quite few.…”

Section: Introductionmentioning

confidence: 99%

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In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview

Elhiti

Stasolla

2010

Methods in Molecular Biology

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Plant propagation in vitro via somatic embryogenesis or organogenesis is a complicated process requiring the proper execution of several steps, which are affected by culture conditions and environment. A key element for a successful outcome is the choice of the explants. Several studies have shown that factors such as age, ontogenic and physiological conditions, and degree of differentiation affect the response of the explants to culture conditions. As a general rule, younger tissues, such as zygotic embryos, are the preferred choice for tissue culturists as they have better potential and competence to produce embryos and organs compared to more differentiated and mature tissues. This chapter focuses on how competence and commitment to regenerate embryos and organs in cultures are acquired by somatic cells and why zygotic embryos are so often utilized for propagation practices.

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