The effect of synthetic surfactant Exosurf on gene transfer in mouse lung in vivo

Abstract

Gene transfer in the lung holds promise for the treatment for 5 days thereafter. When DNA was delivered in a 50% of diseases such as pulmonary fibrosis, cystic fibrosis and suspension of Exosurf, the expression of either CAT or asthma. Pulmonary surfactant has been reported to Luc was significantly reduced by 89.6 ± 1.4% and enhance expression from endobronchial, adenovirus-82.7 ± 10.5%, respectively. The decrease in Luc mediated gene transfer in experimental animals. This study expression was closely correlated (r = 0.99, P Ͻ 0.001) to examines the effect of exogenous synthetic surfactant log concentration of surfactant in the plasmid buffer sol-(Exosurf) on gene expression from naked plasmid DNA ution (IC 50 = 8.6%). CAT expression was not altered when administered endobronchially to adult mice. Transfection surfactant was administered either 2 h before or after plasefficiency was evaluated by quantifying the expression of mid DNA instillation. Examination of the components of chloramphenicol acetyltransferase (CAT) and luciferase Exosurf revealed that two compounds, DPPC and tylox-(Luc) genes in the lung. Endobronchial administration of apol, showed inhibitory effects on CAT expression. Howeither CAT or Luc expression plasmid DNA resulted in ever, the inhibition caused by Exosurf appeared greater detectable concentrations of each reporter protein. CAT than that of either component. Our results suggest that the expression from plasmid DNA was monitored after endolung surfactant is a barrier to transfection of the endobronchial administration with the maximal expression bronchial airway and may be partly responsible for the low observed at 3-5 days after administration and decreasing expression of exogenous DNA in vivo in the bronchial tree.

Substituted β-Cyclodextrins Interact with PAMAM Dendrimer–DNA Complexes and Modify Transfection Efficiency

“…The reporter plasmid pCF1CAT has been described in detail previously (14,15). Plasmid DNA was isolated from bacterial transformants by double cesium chloride gradient and endotoxin levels in each batch of plasmid was determined by limulus assay by the Vector Core Laboratory at the University of Michigan.…”

Gene Transfer Using StarburstTM Dendrimers

“…Preliminary results have indicated that administration by this route may not led to optimal gene expression when compared to 'naked' DNA delivery [78]. Polymerase chain reaction (PCR) confirmed long-term plasmid DNA survival when complexed with dendrimer.…”

Lipopolysaccharide recognition protein, MD‐2, facilitates cellular uptake of E. coli‐derived plasmid DNA in synovium

“…The pCF1-luciferase (pCF1-Luc) plasmid, which contains the cytomegalovirus promoter/enhancer region and firefly (Photinus pyralis) luciferase gene, was purified using an alkaline lysis procedure with the CONCERT plasmid purification system (GibcoBRL, Gaithersburg, MD, USA) according to the manufacturer's instructions [28]. The plasmid was further purified via cesium chloride ultracentrifugation.…”