The Effect of Subconjunctival Platelet-Rich Plasma on Corneal Epithelial Wound Healing

Tanidir, Suzan Tayfun; Yüksel, Nurşen; Altıntaş, Özgül; Yıldız, Demir Kürşat; Sener, Ender; Çağlar, Yusuf

doi:10.1097/ico.0b013e3181c29633

Cited by 36 publications

(35 citation statements)

References 19 publications

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“…PRGF reduces myofibroblast-transformed cells in the corneal stroma (Anitua et al 2013a). Tanidir et al (2010) found similar results in corneal re-epithelization in rabbits. In the same way, successful results were found in a rabbit model of corneal ulcers, showing an acceleration in corneal regeneration, a reduction in ocular inflammation and an improvement in collagen fibre arrangement in the corneal stroma compared to the control group (Khaksar et al 2013).…”

Section: Preclinical Studiessupporting

confidence: 68%

Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies

Anitua

Muruzábal

Tayebba

et al. 2015

Acta Ophthalmologica

134

102

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ABSTRACT. The use of blood derivatives represents an alternative therapeutic approach that is gaining interest in regenerative medicine due to its potential to stimulate and accelerate tissue healing. Autologous serum eye drops and platelet-enriched plasma eye drops are being used in the treatment of different ophthalmological disorders. In this review, we summarize the different blood-derived formulations used in the treatment and care of ocular surface disorders. The biological basis and use of autologous serum and plasma rich in growth factors are deeply evaluated as well as the challenges to be addressed in the future in this new generation of blood-derived therapies.

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Section: Preclinical Studiessupporting

confidence: 68%

Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies

Anitua

Muruzábal

Tayebba

et al. 2015

Acta Ophthalmologica

134

102

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“…In the present study, for the preparation of PRP the method described by Gimeno et al (2006) was followed. The same method has been used in other experimental protocols in rabbits regarding ocular surface conditions (Gimeno et al, 2010;Tanidir et al, 2010;Khaksar et al, 2013). Intracardiac blood sampling was not performed, and blood was drawn from the jugular vein as it can be well tolerated by rabbits, no anaesthesia was required and the resulting blood collection was without clumps.…”

Section: Discussionmentioning

confidence: 99%

“…It has been found that nonactivated PRP to whole blood ratio is 4.6 for EGF and 1.9 for IGF-I (Frechette et al, 2005). PRP has been used experimentally and in clinical trials for the treatment of several ocular conditions, such as corneal epithelial wounds (Tanidir et al, 2010), chemical burns (Khaksar et al, 2013;Márquez-de-Aracena et al, 2007), dry eye (Alio et al, 2007b), corneal ulcers caused by neurotrophic keratitis or trauma (Geremicca et al, 2010) and other ocular surface disorders (Alio et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The effect of intrastromal PRP and oral doxycycline in corneal wound healing after alkali burn

Charalambidou

Kofidou

Papaioannou

et al. 2019

J Hellenic Vet Med Soc

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Ocular chemical burns are among the most critical and true ocular emergencies in dogs and cats, with destructive consequences on the ocular surface and intraocular tissues. Platelet alpha granules are an important reservoir of growth factors that can stimulate chemotaxis of inflammatory cells, mitosis, migration and differentiation of cells. On the other hand, tetracyclines and their derivatives exhibit non-antimicrobial properties, such as affecting inflammation, immunomodulation, cell proliferation and angiogenesis. The objective of this study is to investigate the effect of intrastromal platelet rich plasma (PRP) in combination with oral doxycycline in the healing process of the cornea. Thirty six New Zealand rabbits were used in this experimental study. Alkali burns were created by applying a round filter paper soaked in NaOH. PRP was prepared by centrifugation of autologous blood. The rabbits were allocated to four groups (9 animals each). Group 1 served as the control group without any treatment. Group 2 received doxycycline (5mg/kg b.w.) orally once a day throughout the study. Group 3 received an intrastromal injection of PRP (0.5ml) in the cornea, and group 4 received intrastromal PRP combined with oral doxycycline. Treatments had varied levels of success, with PRP combined with oral doxycycline producing the best results regarding corneal healing. Animals in the control group had epithelial defects for the whole duration of the study, and 3 animals presented corneal perforation. Groups 3 and 4 had smaller mean defect area, compared to groups 1 and 2. On the 7th day, neovascularisation was lower in treatment groups compared to the control group. Groups 3 and 4 also had less corneal oedema compared to the control group on day 3 and 7. Group 4 exhibited the best wound healing, with less neovascularization and better collagen arrangement, as shown by the histopathological evaluation. To the authors’ knowledge this is the first experimental study that intrastromal PRP is combined with oral doxycycline for the management of corneal chemical burns. This combination is a simple, safe and economical therapeutic approach that promotes corneal healing.

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“…78,83–86 These reports include both topical and subconjunctival injection of PRP and suggest it is a safe and effective adjunct to standard medical treatments. The mechanism of action of autologous PRP is likely the same as that of autologous serum.…”

Section: Management Of Acute Phasementioning

confidence: 99%

Current and Upcoming Therapies for Ocular Surface Chemical Injuries

et al. 2017

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Chemical injuries frequently result in vision loss, disfigurement, and challenging ocular surface complications. Acute interventions are directed at decreasing the extent of the injury, suppressing inflammation, and promoting ocular surface re-epithelialization. Chronically, management involves controlling inflammation along with rehabilitation and reconstruction of the ocular surface. Future therapies aimed at inhibiting neovascularization and promoting ocular surface regeneration should provide more effective treatment options for the management of ocular chemical injuries.

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The Effect of Subconjunctival Platelet-Rich Plasma on Corneal Epithelial Wound Healing

Abstract: sPRP seems to improve corneal epithelial wound healing. However, antibiotic and sPRP combination may have a retarded healing effect as compared with platelet-rich plasma alone.

Cited by 36 publications

References 19 publications

Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies

Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies

The effect of intrastromal PRP and oral doxycycline in corneal wound healing after alkali burn

Current and Upcoming Therapies for Ocular Surface Chemical Injuries

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