Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies

Anitua, Eduardo; Muruzábal, Francisco; Tayebba, Ali; Riestra, Ana Cristina; Pérez, Víctor L.; Merayo‐Lloves, Jesús; Orive, Gorka

doi:10.1111/aos.12710

Cited by 138 publications

(115 citation statements)

References 80 publications

(158 reference statements)

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“…Several studies have described the tissue regeneration potential of PRGF eye drops in the ophthalmological field [32][33][34] . One advantage of PRGF compared with other blood derivatives such as autologous serum is its versatility to obtain different therapeutic formulations [35] . Fibrin scaffold is one of these formulations.…”

Section: Discussionmentioning

confidence: 99%

Subconjunctival PRGF Fibrin Membrane as an Adjuvant to Nonpenetrating Deep Sclerectomy: A 2-Year Pilot Study

Rodríguez-Agirretxe

Freire²,

Muruzábal

et al. 2017

Ophthalmic Res

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Purpose: To evaluate the potential role of the autologous PRGF (plasma rich in growth factors) fibrin membrane in tissue regeneration after glaucoma filtering surgery. Materials and Methods: Ten patients with medically uncontrolled primary open-angle glaucoma underwent nonpenetrating deep sclerectomy and were treated with PRGF fibrin membrane as adjuvant. Intraocular pressure reduction was the primary outcome. This variable was measured preoperatively and also at each follow-up visit. Secondary outcomes included the number of antiglaucoma medications, anterior segment optical coherence tomography bleb examination, photographic bleb evaluation, and subjective clinical symptomatology evaluation. Results: The surgical technique showed a significant reduction (p < 0.05) in intraocular pressure in relation to preoperative values at each time of the study, decreasing from 23.3 ± 6.4 to 15.2 ± 4.6 mm Hg at 2 years. Furthermore, the number of antiglaucoma medications consumed showed a significant reduction at the end point of the study compared with the preoperative situation. Optical coherence tomography and photographic filtering bleb variables experienced a progressive reduction during the follow-up. Subjective symptoms showed a reduction from 8.3 ± 4.5 to 4.2 ± 5.3 at 2 years. Conclusions: PRGF-Endoret treatment could promote ocular surface regeneration after glaucoma surgery, enhancing the surgery success rates and reducing the need for postoperative medications. It is important to highlight that this is a preliminary study and some large clinical studies are necessary to verify these results.

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Section: Discussionmentioning

confidence: 99%

Subconjunctival PRGF Fibrin Membrane as an Adjuvant to Nonpenetrating Deep Sclerectomy: A 2-Year Pilot Study

Rodríguez-Agirretxe

Freire²,

Muruzábal

et al. 2017

Ophthalmic Res

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“…Thus, signaling factors in the microenvironment instruct MSCs to remain quiescent, proliferate, migrate, and/or differentiate. In this context, (Anitua et al, 2015) revealed that tfactors released by PRP promote the healing mechanisms of MSCs through microenvironment regulation [47] . Such factors include epidermal growth factor, that is a potent stimulator of epithelial proliferation and migration, exerts an antiapoptotic effect and stimulates extracellular matrix production, including fibronectin and hyaluronic acid.…”

Section: Additionallymentioning

confidence: 99%

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

Ebrahim

Mohammed

Dessouky

et al. 2017

Egyptian Journal of Histology

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Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent visual impairment. In spite of great advances made in the treatment of these cases, the structural and functional restoration of corneal alkali burns remains challenging. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been demonstrated to enhance corneal healing. However the problem remains in finding a suitable vehicle to deliver BM-MSCs to the site of injury. Objectives: To evaluate the therapeutic potential of BM-MSCs loaded on two biological vehicles (amniotic membrane versus platelet-rich plasma gel) in corneal alkali burns. Materials & Methods: Fifty rats were randomly divided into five equal groups. Group I (control group), group II (corneal ulcer group) and group III (recovery group). Group IV rats with corneal ulcers were treated with amniotic membrane loaded with BM-MSCs while in the group V the rats with corneal ulcers were treated with platelet-rich plasma cells loaded with BMMSCs. The corneal specimens were processed for the light microscope, transmission electron microscope examination, and RT qPCR assay for VEGF and iNOS. Results: Group II showed epithelial separation and denudation with disorganized stroma and disrupted Descemet's membrane and endothelium. Group III showed residual epithelial separation and vacuolation with neovascularization of the disorganized stroma. Group IV showed thinning of corneal epithelium with inflammatory infiltration of the stroma. Group V corneas revealed restoration of corneal epithelial thickness, organized stroma and continuous Descemet's membrane. Also, there was an upregulation of iNOS & VEGF gene expression in groups II and III which was downregulated in group IV and V. Conclusions: The combination of BM-MSCs with PRP gel was a promising treatment for corneal alkali burns and may be applicable for other types of corneal disorders.

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“…Many studies have reported that both allogeneic and autologous serum eye drops are effective in alleviating the symptoms of dry eye in different patient cohorts [10][11][12][13][14][15][16][17][18][19], although further randomised controlled trials are warranted due to the limited number of trials and the small number of patients in the studies conducted to date [20]. Others have reported alternative therapies such as albumin [21,22] and eye drops prepared from platelet lysate [23] and platelet rich plasma [24,25] to be effective in treating severe dry eye, as these also contain many growth factors required for epithelial repair and healing [26].…”

Section: Background and History Of Eye Drop Production In Australiamentioning

confidence: 99%

Serum eye drop preparation in Australia: Current manufacturing practice

Marks¹,

Fisher²,

Mondy³

et al. 2015

Transfusion and Apheresis Science

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Autologous serum and plasma rich in growth factors in ophthalmology: preclinical and clinical studies

Cited by 138 publications

References 80 publications

Subconjunctival PRGF Fibrin Membrane as an Adjuvant to Nonpenetrating Deep Sclerectomy: A 2-Year Pilot Study

Subconjunctival PRGF Fibrin Membrane as an Adjuvant to Nonpenetrating Deep Sclerectomy: A 2-Year Pilot Study

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

Serum eye drop preparation in Australia: Current manufacturing practice

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