The effect of strontium incorporation in hydroxyapatite on osteoblasts in vitro

Ni, Guo‐Xin; Yao, Zhipeng; Huang, Guotao; Liu, Wenge; Lu, William W.

doi:10.1007/s10856-011-4264-0

Cited by 64 publications

(49 citation statements)

References 22 publications

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“…SrHA nanoparticles were synthesized by adding Sr(NO 3 ) 2 as dopant to the wet-chemical neutralization reaction between Ca(OH) 2 . Subsequently, the precipitation product was aged for 24 h. All experiments were conducted using freshly prepared suspensions.…”

Section: Methodsmentioning

confidence: 99%

Development of porous polyurethane/strontium‐substituted hydroxyapatite composites for bone regeneration

Sariibrahimoğlu

Yang

Leeuwenburgh

et al. 2014

J Biomedical Materials Res

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Polyurethane (PU) has been widely used for the biomedical applications but its potential for bone regeneration is limited due to its lack of osteoconductive properties. Strontium substituted hydroxyapatite (SrHA) particles, on the other hand, are known to exhibit a positive effect on bone formation. Therefore, the aim of this study was to (i) develop porous polyurethane scaffolds containing strontium SrHA nanoparticles (PU/SrHA) and (ii) compare their in vitro biological performance for applications in bone regeneration to PU scaffolds. SrHA and HA was synthesized using a conventional wet-chemical neutralization reaction at temperatures of 25, 50, and 80°C. Chemical analysis was performed by inductively coupled plasma-optical emission spectrometry. Synthesizing temperatures at 25 and at 50°C were selected for the composite preparation (abbreviated as HA-25, SrHA-25, HA-50, and SrHA-50, respectively). PU was synthesized from isophorone diisocyanate, polytetramethylene ether glycol, and 1,4-butanediol. Composite scaffolds were prepared by addition of HA or SrHA nanoparticles into PU scaffolds during polymer preparation. The results showed that the Sr content in HA nanoparticles increased with increasing synthesis temperature. The addition of nanoparticles decreased the elongation-at-break and tensile strength, but significantly increased the surface wettability of the PU scaffolds. In vitro degradation tests demonstrated that release of cations was significantly higher from PU/SrHA-50 composite scaffolds. Cell culture tests indicated that PU composites containing either HA or SrHA nanoparticles increased proliferation of bone marrow stem cells as compared to plain PU scaffolds, whereas osteogenic differentiation was not affected by the incorporation of HA nanoparticles irrespective of the incorporation of Sr.

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Section: Methodsmentioning

confidence: 99%

Development of porous polyurethane/strontium‐substituted hydroxyapatite composites for bone regeneration

Sariibrahimoğlu

Yang

Leeuwenburgh

et al. 2014

J Biomedical Materials Res

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“…Zn-HA showed enhanced osteoblast proliferation and differentiation as a coating on porous titanium surfaces , as well as antimicrobial activity (Stanić et al 2010). Sr-HA promoted osteogenic activity and mineralisation in osteoblasts and inhibited the proliferation of osteoclasts, and these effects were more prominent at higher strontium contents (Capuccini et al 2009;Ni et al 2011). Mg-HA demonstrated improved osteoconductivity and resorption compared to stoichiometric hydroxyapatite as bone fillers in a rabbit model (Landi et al 2008).…”

Section: Designs To Mimic Mineral Phase Of Bonementioning

confidence: 96%

Bone-Biomimetic Biomaterial and Cell Fate Determination

Zreiqat

2014

Mechanical Engineering Series

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Despite its remarkable capacity to undergo self-repair, bone tissue cannot regenerate across critical-sized defects, and their successful reconstruction remains a major clinical challenge. Current treatment options are limited and often associated with a high incidence of complications, which may result in non-union or re-fracture. There is a great and growing need for alternative techniques to replace, restore or regenerate damaged or diseased bone. Biomaterials-based bone tissue engineering via the use of synthetic bone substitutes represents a particularly promising alternative, which circumvents the drawbacks of conventional treatments. To achieve successful reconstructive outcomes, synthetic bone substitutes need to be biocompatible and provide necessary signals to osteoprogenitor cells to control downstream cell responses including adhesion, migration, proliferation and differentiation into osteoblasts. One feasible approach to develop synthetic bone substitutes with such biological properties is to mimic the innate physical and/or chemical properties of bone. In this chapter, we discuss the design aspects of bone-biomimetic biomaterials that provide the signals necessary for bone regeneration, and the underlying mechanisms by which bone-biomimetic biomaterials determine the fate of mesenchymal stem cells/osteoprogenitor cells. Protein adsorption to biomaterial surfaces and their subsequent influence on cell adhesion and intracellular signal transduction will be discussed in detail, with particular emphasis on the key molecules and signalling pathways involved in directing the osteogenic development of cells.

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“…3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the metabolic condition of the cells (Ni et al, 2011). After culturing for 1, 6, and 24 h, MTT (5 mg/ml) (Sigma Aldrich, St. Louis, MO, USA) was added to each well and the cells were cultured for another 4 h. After the culture medium was removed, 100 μl dimethyl sulfoxide (DMSO; Sigma Aldrich) was added to the well and oscillated for 10 min.…”

Section: Cell Viabilitymentioning

confidence: 99%

Adhesion of bone marrow mesenchymal stem cells on porous titanium surfaces with strontium-doped hydroxyapatite coating

Jiang

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To determine the adhesion behavior of bone marrow mesenchymal stem cells (MSCs) on a titanium surface with a nanostructured strontium-doped hydroxyapatite (Sr-HA) coating. Methods: Sr-HA coatings were applied on roughened titanium surfaces using an electrochemical deposition method. Primary cultured rat MSCs were seeded onto Sr-HA-, HA-coated titanium, and roughened titanium surfaces, and they were then cultured for 1, 6, and 24 h. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the metabolic condition of the cells. Scanning electron microscopy (SEM) was used to observe the cell morphology. The cytoskeletal structure was analyzed using fluorescence actin staining to characterize cell adherence. Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) was used to analyze the gene expression levels of FAK (focal adhesion kinase), vinculin, integrin β1, and integrin β3 after culturing for 24, 48, and 72 h. Results: MSCs cultured on the Sr-HA surface showed better cell proliferation and viability. Improvement of cell adhesion and structural rearrangement of the cytoskeleton were observed on the Sr-HA surface. The gene expression of FAK, vinculin, integrin β1, and integrin β3 was also elevated on the Sr-HA surface. Conclusions: Cell viability, adhesion, cell morphology, and the cytoskeletal structure were all upregulated considerably by the titanium surface modified with a Sr-HA coating.

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The effect of strontium incorporation in hydroxyapatite on osteoblasts in vitro

Cited by 64 publications

References 22 publications

Development of porous polyurethane/strontium‐substituted hydroxyapatite composites for bone regeneration

Development of porous polyurethane/strontium‐substituted hydroxyapatite composites for bone regeneration

Bone-Biomimetic Biomaterial and Cell Fate Determination

Adhesion of bone marrow mesenchymal stem cells on porous titanium surfaces with strontium-doped hydroxyapatite coating

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