The effect of steam sterilization on recombinant spider silk particles

Lucke, Matthias; Winter, Gerhard; Engert, Julia

doi:10.1016/j.ijpharm.2015.01.024

Cited by 13 publications

(14 citation statements)

References 34 publications

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“…In a recent work, spider silk particles have been sterilized by autoclave. Lucke et al showed that the combination of high temperature, humidity and pressure did not affect the secondary structure of eADF4(C16) particles [29]. In order to analyze potential differences in hydration behavior of the films, water absorption and desorption isotherms were collected by IGASorp (Figure 3).…”

Section: Preparation and Characterization Of Eadf4(c16) Water-based Fmentioning

confidence: 99%

Water-based preparation of spider silk films as drug delivery matrices

Agostini

Winter

Engert

2015

Journal of Controlled Release

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Section: Preparation and Characterization Of Eadf4(c16) Water-based Fmentioning

confidence: 99%

Water-based preparation of spider silk films as drug delivery matrices

Agostini

Winter

Engert

2015

Journal of Controlled Release

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“…In addition, further reducing the size and size distribution can probably be achieved by raising the temperature of the mixed solutions. The eADF4(C16) spheres formed at 60°C were smaller in size than the spheres obtained at room temperature with the same mixing parameters . In addition, the micromixing system significantly facilitated and accelerated the production process of the particles.…”

Section: Discussionmentioning

confidence: 94%

“…The eADF4(C16) spheres formed at 60 C were smaller in size than the spheres obtained at room temperature with the same mixing parameters. 38 In addition, the micromixing system significantly facilitated and accelerated the production process of the particles. It not only was adapted for large-scale sphere preparation but also incorporated a rapid and efficient method for sphere recovery from a high molar salt solution based on ultrafiltration, providing large amounts of the carrier in a short time.…”

Section: Discussionmentioning

confidence: 99%

Optimization of spider silk sphere formation processing conditions to obtain carriers with controlled characteristics

Florczak

Jastrzębska

Białas

et al. 2018

J Biomedical Materials Res

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Bioengineered spider silk is a focus of research due to its biocompatibility, biodegradability, and excellent mechanical properties. Functionalized silk can be processed into spheres and employed as selective drug delivery vehicles in targeted cancer treatment. Efficient, repeatable and controllable processing conditions are essential to drug carrier development. This study aimed to optimize the processing conditions of silk spheres formation, scale‐up, and automation of the silk spheres production process. The automated micromixing system provided substantial amounts of silk spheres under repetitive production conditions. Micromixing resulted in smaller sphere sizes and narrower sphere size distributions than mixing with a pipette. Furthermore, the particle size and size distribution of silk spheres could be tailored by varying mixing process parameters, such as protein concentration, silk and salting out buffer ratio, mixing speed, and the size of the tubes and mixing zone. In addition, the implementation of ultrafiltration techniques provided a fast and efficient concentration of spheres in water. Furthermore, the shear forces introduced by micromixing did not impede the properties of the Her2 binding peptide (H2.1) since the functionalized H2.1MS1:H2.1MS2 silk spheres selectively were internalized by Her2‐positive cancer cells. This study indicated that micromixing in combination with ultrafiltration enabled scale‐up of the sphere production process under controllable and repeatable conditions. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3211–3221, 2018.

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“…Lucke et al showed that spider silk nanostructured hydrogel was resistant to SH sterilization and revealed increased thermal stability after autoclave treatment.…”

Section: Sterilization Of Hydrogel‐based Medical Devicesmentioning

confidence: 99%

“…A recent review regarding the sterilization of nanoparticles was published by Vetten et al 16 Although several cited authors used hydrophilic polymers such as PEG, collagen and poly(lactic-co-glycolic acid) (PLGA), there is no reference to the possible hydrogel character of such structures. 85,86 Lucke et al 87 showed that spider silk nanostructured hydrogel was resistant to SH sterilization and revealed increased thermal stability after autoclave treatment.…”

Section: Sterilization Of Micro/nanostructured Hydrogel Systemsmentioning

confidence: 99%

Sterilization of hydrogels for biomedical applications: A review

et al. 2017

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Despite the beneficial properties and outstanding potential of hydrogels for biomedical applications, several unmet challenges must be overcome, especially regarding to their known sensitivity to conventional sterilization methods. It is crucial for any biomaterial to withstand an efficient sterilization to obtain approval from regulatory organizations and to safely proceed to clinical trials. Sterility assurance minimizes the incidence of medical device-related infections, which still constitute a major concern in health care. In this review, we provide a detailed and comprehensive description of the published work from the past decade regarding the effects of sterilization on different types of hydrogels for biomedical applications. Advances in hydrogel production methods with simultaneous sterilization are also reported. Terminal sterilization methods can induce negative or positive effects on several material properties (e.g., aspect, size, color, chemical structure, mechanical integrity, and biocompatibility). Due to the complexity of factors involved (e.g., material properties, drug stability, sterilization conditions, and parameters), it is important to note the virtual impossibility of predicting the outcome of sterilization methods to determine a set of universal rules. Each system requires case-by-case testing to select the most suitable, effective method that allows for the main properties to remain unaltered. The impact of sterilization methods on the intrinsic properties of these systems is understudied, and further research is needed. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2472-2492, 2018.

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The effect of steam sterilization on recombinant spider silk particles

Cited by 13 publications

References 34 publications

Water-based preparation of spider silk films as drug delivery matrices

Water-based preparation of spider silk films as drug delivery matrices

Optimization of spider silk sphere formation processing conditions to obtain carriers with controlled characteristics

Sterilization of hydrogels for biomedical applications: A review

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