The effect of soursop‐flower‐enriched fried palm olein on some biochemical and hematological parameters of rats

Abstract

This work set out to, first, assess the role of soursop flower extracts (SFE) in limiting palm olein oxidation during the production of plantain chips, before ascertaining the effect of these soursop‐flower‐enriched fried palm olein on some biochemical and hematological parameters of rats. The extracts were added to 1.5 kg of oil at 1000, 1400, and 1800 ppm, while BHT at 200 ppm served as a positive control (PO+BHT), and the oil without additives was the negative control (PO). The samples were subjected to 15 frying cycles. Total oxidation values varied between 5.94 ± 0.0 and 31.58 ± 0.37; 8.08 ± 0.25 and 28.24 ± 0.00 and 13.71 ± 0.24 and 42.71 ± 0.40 respectively for palm olein enriched with SFE, for PO+BHT and for PO. Twenty‐one groups each comprising five rats received, through dietary supplementation, oils subjected to 0, and 5, 10 and 15 frying cycles for a duration of 30 days. The alanine transaminase and aspartate transaminase of rats fed with oils enriched with SFE at fresh states and at 5 frying cycles was comparable to that of the neutral control group (23.45 ± 2.65 and 93.10 ± 3.53 U/L) and lower than that of the negative control group (52.15 ± 2.01 and 124.07 ± 1.89 U/L). The HDL cholesterol of these animals was also comparable to that of the neutral control group (67.82 ± 4.06 mg/dl) and higher than that of the negative control group (50.25 ± 5.20 mg/dl). White blood cells and mean corpuscular volume of rats fed with fried olein previously enriched with SFE were lower than those fed with fried olein without additives. These extracts are recommended as natural antioxidants for the stabilization of palm olein.

Role of TLR4 signaling pathway in the mitigation of damaged lung by low‐dose gamma irradiation

“…At the end of the experiment, whole blood samples were collected by heart puncture from each animal under anesthesia with urethane (1.2 g/kg body weight; Sigma-Aldrich) as reported by Aboumsallem et al 25 in clean, sterile test tubes containing ethylenediamine tetraacetic acid anticoagulant for apoptosis and hematological studies. 26 Lung tissues were isolated, washed with isotonic ice-cold saline, dried on filter paper, and weighed immediately. Part of the lung tissue was homogenized in 50 mM cold phosphate buffer (pH 7.4).…”

“…Whole blood (3 mL) was analyzed using an automated blood cell counter (Techo) to measure various hematological parameters, including white blood cells (WBCs; ×10 3 μL/1) LYM (%), red blood cell levels (RBCs; ×10 6 μL/1) hemoglobin concentration (HGB; g/dL) platelet count (PLT; ×10 3 μL/1), and hematocrit percentage (Hct; %). 26,28…”