The effect of simultaneous exposure of human fibroblasts to fluoride and moderate intensity static magnetic fields

Kimsa-Dudek, Magdalena; Synowiec-Wojtarowicz, Agnieszka; Derewniuk, Małgorzata; Paul-Samojedny, Monika; Pawłowska-Góral, Katarzyna

doi:10.1080/09553002.2019.1642543

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…This work complements our previous works on the effects of fluoride on cell systems [11,12,23]. The toxicity of fluorine compounds has been proven and the results of the research have been widely described [24][25][26].…”

Section: Discussionsupporting

confidence: 68%

“…In addition, in a study of men, Sirmatel et al [10] found a decrease in the oxidative stress index (OSI) and total oxidant status (TOS) after exposure to a SMF. A similar SMF effect was also observed at the cellular level [11,12].…”

Section: Introductionsupporting

confidence: 75%

“…The research was conducted with magnetic field intensities that were parametrically changed by altering the distance from the permanent magnets. In addition, a control was used in which steel was employed instead of a magnet (0.0 T) [11,12,19,20]. A Gauss meter was used to assess the flux densities.…”

Section: Cell Culturementioning

confidence: 99%

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The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

2020

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A redox imbalance disrupts the cellcycle and the proliferation process, and contributes to the initiation of programmed cell death. One of the pathways that are important for redox homeostasis is the Nrf2-ARE signaling pathway. Fluoride as well as static magnetic fields (SMF) are associated with the concepts of oxidative stress, and thus programmed cell death. Therefore, this study aimed to assess the connection between oxidative stress and apoptosis in human cells co-exposed to fluoride and a SMF with a different magnetic induction and to determine whether the Nrf2-signaling pathway is involved in these effects. The research was realized using normal human dermal fibroblasts that had been co-exposed to fluoride (0.3 mmol/L) and a SMF with a different magnetic induction (0.45 T, 0.55 T, 0.65 T) for 12 h. The mRNA levels of the cellular antioxidant system-related genes and apoptosis-related genes were assessed using the quantitative reverse transcription polymerase chain reaction (RT-qPCR) method. Our results indicated that the increased activity of antioxidant enzymes (SOD1 (superoxide dismutase 1), SOD2 and GSR (glutathione reductase)) suggests the restoration of the cell redox homeostasis that had been disturbed by fluoride, and also that the genes whose expression is associated with the induction of apoptosis are down regulated as a result of exposure to a SMF. The SMF with a 0.65 T flux density had the strongest effect on the fibroblasts. Moreover, our findings demonstrated that the Nrf2 transcription factor plays a crucial role in the protective effect of a SMF against fluoride toxicity in human cells. The results of these studies can form the basis for developing therapeutic strategies for apoptosis and oxidative stress-related diseases.

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Section: Discussionsupporting

confidence: 68%

Section: Introductionsupporting

confidence: 75%

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The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

2020

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“…For the experiment, the fibroblast cultures were conducted in the presence of fluoride (0.3 mmol/L) and simultaneously exposed to a static magnetic field with a moderate magnetic induction (0.65 T) for 24 h. The static magnetic field was generated by permanent magnets in special magnetic chambers that are intended for in vitro research [19,20]. The control cultures were carried out without fluoride and in chambers with a magnetic induction of 0.0 T. The concentration of fluoride and the value of the magnetic induction were selected based on previous studies that showed that these agents had no cytotoxic effect on cells [16,21,22]. Moreover, the concentration of F − was selected so that the precipitation of sparingly soluble calcium and magnesium fluorides did not occur.…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field

et al. 2021

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Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F− and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (IL27RA, NR1D1, RRP7A, YIPF1, HIST1H2BD) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.

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Static Magnetic Field Reduces Intracellular ROS Levels and Protects Cells Against Peroxide-Induced Damage: Suggested Roles for Catalase

Frachini,

Silva,

Fornaciari

et al. 2023

Neurotox Res

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The effect of simultaneous exposure of human fibroblasts to fluoride and moderate intensity static magnetic fields

Cited by 5 publications

References 36 publications

The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

The Protective Effect of Static Magnetic Fields with Different Magnetic Inductions against Fluoride Toxicity Is Related to the NRF2 Signaling Pathway

Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field

Static Magnetic Field Reduces Intracellular ROS Levels and Protects Cells Against Peroxide-Induced Damage: Suggested Roles for Catalase

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