The effect of semen processing on sperm DNA integrity: comparison of two techniques using the novel Toluidine Blue Assay

Marchesi, Dennis; Biederman, Hannah; Ferrara, Scott; Hershlag, Avner; Feng, Huai L.

doi:10.1016/j.ejogrb.2010.05.003

Cited by 36 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The quality of the sperm sample is influenced by various laboratory factors, including: (i) use of different sperm preparation techniques (Boomsma et al, 2007;Chen and Bongso, 1999;Marchesi et al, 2010); (ii) temperature during sperm preparation (Franken et al, 2011;Otsuki et al, 2008); (iii) time interval from sperm preparation to IUI (Yavas and Selub, 2004); and (iv) temperature during long-term in-vitro incubation of prepared sperm samples (Aitken et al, 1996;Makler et al, 1981;Matsuura et al, 2010;Petrella et al, 2003). It is well known that the testis temperature is approximately 2-3°C below body temperature (Elder and Dale, 2011), as this is required for the production and maintenance of viable spermatozoa (Appell et al, 1977;Setchell, 1998).…”

Section: Introductionmentioning

confidence: 99%

Influence of temperature and sperm preparation on the quality of spermatozoa

Thijssen

Klerkx

Huyser

et al. 2014

Reproductive BioMedicine Online

View full text Add to dashboard Cite

.be (A Thijssen).Annelies Thijssen graduated in July 2012 with a Masters in biomedical sciences -clinical molecular sciences from the Transnational University Limburg, Diepenbeek, Belgium. She finished her master thesis on 'Methods for optimal sperm selection and preservation in the IVF laboratory' at the Genk Institute for Fertility Technology of the Ziekenhuis Oost-Limburg, Genk, Belgium. In September 2012, she started a PhD project 'Sperm banking in Belgium: medical, ethical and economical aspects', a collaboration between Hasselt University and the Ziekenhuis Oost-Limburg with Willem Ombelet as promoter.Abstract This study investigated the effects of long-term (24 h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n = 41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influence of sperm preparation on sperm quality after incubation. Progressive motility and morphology were significantly higher after incubation at RT compared with 35°C( P < 0.001 and P < 0.01, respectively). The proportions of acrosome-reacted, apoptotic and dead spermatozoa were significantly lower in samples incubated for 24 h at RT compared with 35°C( P < 0.001, P = 0.01 and P < 0.001, respectively). The number of motile, morphologically normal, non-acrosome-reacted and nonapoptotic spermatozoa recovered after sperm preparation was significantly higher in DGC compared with swim-up samples (P < 0.001). However, spermatozoa prepared by swim-up showed better survival after incubation compared with DGC-prepared spermatozoa, especially when incubated at 35°C. In conclusion, this study indicates a significantly better and longer preservation of sperm quality when incubation is performed at RT. These findings may convince laboratories to change the routinely used sperm storage conditions in order to maximize the quality of the prepared sperm sample.

show abstract

Section: Introductionmentioning

confidence: 99%

Influence of temperature and sperm preparation on the quality of spermatozoa

Thijssen

Klerkx

Huyser

et al. 2014

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…Fortunately, in humans, the risk of injecting DNA damaged spermatozoa seems to be minimized by classical sperm preparation techniques prior to ICSI (Zini et al 2000;Younglai et al, 2001;Donnelly et al, 2000;Ahmad et al, 2007;Jackson et al, 2010;Marchesi et al, 2010;Castillo et al, 2011: Ebner et al, 2011 and follow-up studies on ICSI children have demonstrated the safety of this technique (Van Steirteghem et al, 2002, Leunens et al, 2008Belva et al, 2011;Woldringh et al, 2011) although a slight increase of chromosome aberration seems to be caused by the injection of aneuploid spermatozoa .…”

Section: Discussionmentioning

confidence: 99%

New Advances in Intracytoplasmic Sperm Injection (ICSI)

Parmegiani¹,

Cognigni²,

Filicori³

2012

Advances in Embryo Transfer

View full text Add to dashboard Cite

“…As spermatozoa have no mechanism to repair DNA damage, DNA strand breaks can be transferred to the oocyte during ICSI, and will rely on the oocyte to repair them (Lewis and Kumar, 2015). Therefore, sperm with higher levels of DNA fragmentation may contribute to embryonic mortality (Bonduelle et al, 2002;Jakab et al, 2005;Heytens et al, 2009;Marchesi et al, 2010). Hence, new methods to select sperm of optimum quality for use in assisted reproduction techniques are still being developed, including morphological (The International Mobile Subscriber Identity), electrical (flow cell, Zeta potential) and cell sorting methods (fluorescent-activated cell sorting and magnetic-activated cell sorting) (Chan et al, 2006;Nasr-Esfahani et al, 2012;Delaroche et al, 2013;Sakkas, 2013;Teixeira et al 2013) Previous studies have suggested that sperm able to bind the endocervical mucus have better morphology and that selecting for sperm with good morphology, inter alia should also select for sperm with other good parameters, including progressive motility and low DNA fragmentation (Sati et al, 2008;Prinosilova et al, 2009;Yagci et al, 2010).…”

Section: Changes In Sperm Hyaluronic-acid-binding and Hyperactive Motmentioning

confidence: 99%

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

Torabi

Binduraihem

Miller

2017

Reproductive BioMedicine Online

View full text Add to dashboard Cite

ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. Accepted ManuscriptTitle: Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid 22-11-2016 Please cite this article as: Forough Torabi, Adel Binduraihem, David Miller, Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid, Reproductive BioMedicine Online (2016), http://dx.doi.org/doi: 10.1016/j.rbmo.2016.11.011. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins.Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronan acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation Overall success rates for assisted conception procedures, however (including ICSI), have remained relatively static, with about 24% of cycles for all age groups deliverin...

show abstract

The effect of semen processing on sperm DNA integrity: comparison of two techniques using the novel Toluidine Blue Assay

Cited by 36 publications

References 23 publications

Influence of temperature and sperm preparation on the quality of spermatozoa

Influence of temperature and sperm preparation on the quality of spermatozoa

New Advances in Intracytoplasmic Sperm Injection (ICSI)

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

Contact Info

Product

Resources

About