Influence of temperature and sperm preparation on the quality of spermatozoa

Thijssen, Annelies; Klerkx, E; Huyser, Carin; Bosmans, Eugène; Campo, Rudi; Ombelet, Willem

doi:10.1016/j.rbmo.2013.12.005

Cited by 41 publications

(30 citation statements)

References 33 publications

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“…However, the survival rate of spermatozoa at RT did not significantly decline. In agreement with our data, Thijssen et al () showed that incubation at RT resulted in significantly less apoptotic and dead spermatozoa compared with testis temperature (35°C). Incubation at 37°C decreased sperm viability and motility to a greater extent than incubation at RT.…”

Section: Discussionsupporting

confidence: 93%

“…Epigenetic spermatozoa abnormalities such as aberrant DNA methylation patterns and reprogramming errors can impact both fertilisation rate and pre‐implantation embryo development (Emery & Carrell, ). Previous studies have shown that incubation of spermatozoa at various temperatures affects spermatozoa parameters such as motility (Matsuura et al, ), survival (Thijssen et al, ) and reactive oxygen species (ROS) levels (Esfandiari et al, ). However, no published study has addressed the changes in DNA methylation after extended incubation.…”

Section: Introductionmentioning

confidence: 99%

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DNA integrity and methylation changes of mouse spermatozoa following prolonged incubation

et al. 2019

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Sperm quality can be affected by different factors including the length of incubation time between sperm preparation and intracytoplasmic sperm injection. Here, we have evaluated the level of DNA methylation and expressions of related genes in mice spermatozoa. The spermatozoa were divided into three groups: fresh, spermatozoa incubated at room temperature (RT) and 37°C for 24 hr. The sperm chromatin structure assay was used to determine the DNA fragmentation index (DFI), and DNA methylation was analysed by flow cytometry. The expression levels of DNA methylation‐related genes were determined by quantitative real‐time PCR (qRT‐PCR). According to the results, we observed significantly higher sperm progressive motility and viability in the group incubated at RT compared to the spermatozoa incubated at 37°C (p < 0.05). Spermatozoa incubated at 37°C had a higher DFI compared to the other groups (p < 0.05), but the DNA methylation level significantly decreased (p < 0.05). qRT‐PCR analysis showed increased Dnmt‐1 expression in spermatozoa after 24‐hr incubation at 37°C. However, there were significantly higher expression levels of Dnmt‐3l, Dnmt‐3a and Dnmt‐3b after incubation at both RT and 37°C compared to the fresh group (p < 0.05). The 24‐hr incubation period affected both sperm DNA methylation and integrity. This study indicated that incubation at RT resulted in better sperm quality.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

DNA integrity and methylation changes of mouse spermatozoa following prolonged incubation

et al. 2019

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“…Thijssen et. al and Petrella et al reported and also we observed in our study, that the motility of washed sperm decreases with prolonged incubation at both room temperature and at 37°C [2,16]. We attribute the more severe motility decrease observed at 37°C to the faster biological reactions as an effect of temperature, and it is a phenomenon consistent with the literature [17].…”

Section: Discussionsupporting

confidence: 92%

The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm

Demirci¹,

Kervancioğlu²,

Bekiroglu³

et al. 2017

Marmara Medical Journal

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Objective: Fertilization capacity of capacitated sperm decreases exponentially over time, but the reason is still under investigation. The aim of the study was to analyze the effect of prolonged incubation and temperature on sperm fertilization capacity with motility and staining parameters of phospholipase C-zeta (PLCZ), which is considered to be the oocyte activation factor.Materials and Methods: Density gradient washing was applied to semen of 11 infertile patients without severe oligoasthenospermia out of 16 patients. The samples were divided and cultured either at room temperature or at 37°C for 3 days. The spermatozoons were evaluated for motility, PLCZ staining and intensity daily.Results: All parameters decreased both at room and body temperature with increased incubation time. There was a strong correlation between the change in motility and in the percentage of PLCZ stained sperms, but this correlation decreased with incubation time.Conclusion: Prolonged incubation results show the correlation between PLCZ staining parameters and motility. Routine use of PLCZ staining together with semen analysis, will be useful to predict fertilization capacity of the sperm especially for unexplained infertility and fertilization failure cases, and also can increase the success of assisted reproductive technologies (ART) cycles. Keywords: Temperature, Phospholipase C-zeta, Fertilization capacity, Sperm, Oocyte activaton, Prolonged incubation ÖZ Amaç: Kapasite olmuş spermin fertilizasyon kapasitesinin zamanla üstsel olarak düştüğü bilinmesine rağmen bunun nedeni hala araştırılmaktadır. Çalışmada amaç; uzamış inkübasyonun ve sıcaklığın sperm fertilizasyon kapasitesi üzerinde etkisini hareketlilik ve oosit aktivasyon faktörü olarak kabul edilen fosfolipaz C-zeta (PLCZ) ile değerlendirmektir.Gereç ve Yöntem: On altı infertil erkek hastadan, ciddi oligoasthenospermisi olmayan 11'inin semenlerine dansite gradyan yıkaması uygulandı. Ardından örnekler bölünerek 3 gün oda sıcaklığında veya 37°C'de kültüre edildi. Örnekler hareketlilik, PLCZ boyanma yüzdesi ve yoğunluğu açısından günlük olarak değerlendirildi.Bulgular: Hem oda sıcaklığı, hem de vücut sıcaklığında her üç parametrede de zamanla azalma gözlendi. Özellikle günler arası hareketlilikteki değişim ile PLCZ boyanan sperm yüzdesindeki değişim arasında güçlü bir korelasyon bulundu, ancak bu korelasyon zamanla azalmakta idi.Sonuç: İnkübasyon süresinin uzamasıyla, PLCZ boyanma parametreleri ve hareketlilik arasında korelasyon görülmektedir. PLCZ boyanmasının semen analizi ile rutinde kullanımı, özellikle açıklanamayan infertilite ve fertilizasyon başarısızlığı vakalarının spermlerinin fertilizasyon kapasitesinin belirlenmesinde yarar sağlayabilecek ve yardımcı üreme teknileri (YÜT) sikluslarındaki başarıyı artırabilecektir.

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“…The present study demonstrated a significantly better and longer retention of spermatozoa motility when washed samples were incubated at room temperature (22°C) for 24 hrs compared to the control held at 37°C, and at other holding temperatures in both protein-free and protein-containing culture media. This finding was supported by the observations of Thijssen (Thijssen et al 2014) who observed better spermatozoa quality in washed spermatozoa samples incubated at room temperature than in those incubated at 37°C. The attempt to retain optimal spermatozoa motility over a short period of time by reducing the holding temperature to 15°C proved to be of no benefit.…”

Section: Discussionsupporting

confidence: 80%

Characteristics of Human Spermatozoa Harvested in Culture Media with and Without Serum Proteins

Ata’Allah

Adenan²,

Razali³

et al. 2018

JSKM

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This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n = 71) were equally apportioned and washed using synthetic protein-free medium (PFM), minimum essential medium + HSA (MEM) or commercial protein-containing medium (CPC). Washed spermatozoa were cultured in vitro using PFM, MEM or CPC media and held for 24 hrs at 4°C, 15°C, 22°C or 37°C. Spermatozoa activity was evaluated at 0 hr, 4 to 7 hrs and 24 hrs post-wash. The effects of PFM on spermatozoa motility, vitality, membrane integrity and DNA fragmentation level were not significantly different from that of MEM and CPC media at 0 hr, 4 to 7 hrs and 24 hrs post-wash in vitro. Synthetic PFM, MEM and CPC retained spermatozoa activity highest when specimen were held at 22°C and it was significantly higher (p < 0.05) than that at 37°C after 24 hrs incubation in vitro. However, no significant changes (p > 0.05) were noted in spermatozoa DNA fragmentation (SDF) levels when specimen were held at 22°C or 37°C at 4 to 7 hrs and also after 24 hrs post-wash in vitro in all media. The use of synthetic PFM as an alternative to the commercial protein-containing media in human spermatozoa washing and preparation procedure for an efficient and safer (Assisted Reproduction Technology) ART outcome. Spermatozoa activity can be successfully retained at room temperature post-wash over short periods; spermatozoa may lose viability rapidly if held for long hours at 37°C in all media. ABSTRAKKajian ini bertujuan untuk menentukan keberkesanan media bebas protein sintetik bagi pencucian spermatozoa, penyediaan dan penahanan aktiviti spermatozoa cucian dalam jangka masa terhad, secara in vitro. Sampel semen normozoospermik (n = 71) dibahagi-bahagikan secara sama rata dan dicuci dengan menggunakan medium bebas protein sintetik (PFM), medium esensial minimum + HSA (MEM) ataupun medium terkandung protein komersial (CPC). Spermatozoa cucian dikultur secara in vitro dengan menggunakan media PFM, MEM atau CPC dan ditahan selama 24 jam pada suhu 4°C, 15°C, 22°C atau 37°C. Aktiviti spermatozoa telah dinilai pada sela masa pascacucian 0 jam, 4 hingga 7 jam dan 24 jam. Kesan PFM terhadap motiliti, kevitalan, integriti membran dan tahap fragmentasi DNA tidak menunjukkan perbezaan signifikan berbanding kesan media MEM dan CPC pada sela masa 0 jam, 4 hingga 7 jam dan 24 jam pascacucian secara in vitro. PFM sintetik, MEM dan CPC mengekalkan aktiviti tertinggi bagi spermatozoa apabila spesimen ditahan pada suhu 22°C, dan aktiviti ini secara signifikan adalah lebih tinggi (p < 0.05) pada suhu 37°C selepas inkubasi secara in vitro selama 24 jam. Walau bagaimanapun, tiada perubahan signifikan (p > 0.05) diperhatikan terhadap tahap fragmentasi DNA spermatozoa (SDF) apabila spesimen ditahan pada suhu 22°C atau 37°C antara 4 hingga 7 jam dan juga selepas 24 jam proses pascacucian in vitro dilakukan dalam semua media. Penggunaan PFM si...

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Influence of temperature and sperm preparation on the quality of spermatozoa

Cited by 41 publications

References 33 publications

DNA integrity and methylation changes of mouse spermatozoa following prolonged incubation

DNA integrity and methylation changes of mouse spermatozoa following prolonged incubation

The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm

Characteristics of Human Spermatozoa Harvested in Culture Media with and Without Serum Proteins

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