The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Lee, Sang Cheol; Lee, Nahyun; Patel, Kapil D.; Jang, Tae-Su; Knowles, Jonathan C.; Kim, Hae‐Won; Lee, Hae‐Hyoung; Lee, Jung Hwan

doi:10.3390/nano11020557

Cited by 21 publications

(20 citation statements)

References 69 publications

(37 reference statements)

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“…SeNPs and SeNWs were selectively synthesized by reducing aqueous solutions of sodium selenite with ascorbic acid and glucose respectively, as previously reported [45,46]. In a typical procedure of synthesizing SeNPs, 100 mM of sodium selenite aqueous solution was mixed with 100 mM of ascorbic acid, and the solution pH was adjusted to 7.1 using NaOH or HCl.…”

Section: Synthesis Of Senps and Nanowiresmentioning

confidence: 99%

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

et al. 2021

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In recent years, multidrug-resistant (MDR) bacteria have increased rapidly, representing a major threat to human health. This problem has created an urgent need to identify alternatives for the treatment of MDR bacteria. The aim of this study was to identify the antibacterial activity of selenium nanoparticles (SeNPs) and selenium nanowires (SeNWs) against MDR bacteria and assess the potential synergistic effects when combined with a conventional antibiotic (linezolid). SeNPs and SeNWs were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), zeta potential, and UV-visible analysis. The antibacterial effects of SeNPs and SeNWs were confirmed by the macro-dilution minimum inhibitory concentration (MIC) test. SeNPs showed MIC values against methicillin-sensitive S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), vancomycin-resistant S. aureus (VRSA), and vancomycin-resistant enterococci (VRE) at concentrations of 20, 80, 320, and >320 μg/mL, respectively. On the other hand, SeNWs showed a MIC value of >320 μg/mL against all tested bacteria. Therefore, MSSA, MRSA, and VRSA were selected for the bacteria to be tested, and SeNPs were selected as the antimicrobial agent for the following experiments. In the time-kill assay, SeNPs at a concentration of 4X MIC (80 and 320 μg/mL) showed bactericidal effects against MSSA and MRSA, respectively. At a concentration of 2X MIC (40 and 160 μg/mL), SeNPs showed bacteriostatic effects against MSSA and bactericidal effects against MRSA, respectively. In the synergy test, SeNPs showed a synergistic effect with linezolid (LZD) through protein degradation against MSSA and MRSA. In conclusion, these results suggest that SeNPs can be candidates for antibacterial substitutes and supplements against MDR bacteria for topical use, such as dressings. However, for use in clinical situations, additional experiments such as toxicity and synergistic mechanism tests of SeNPs are needed.

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Section: Synthesis Of Senps and Nanowiresmentioning

confidence: 99%

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

et al. 2021

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“…SeNPs were synthesized by the reduction method, as previously reported by our group [ 26 ]. Briefly, an aqueous solution of sodium selenite (100 mM) was mixed with an aqueous solution of ascorbic acid (100 mM) using magnetic stirring, and the pH was maintained at 7.1 using NaOH (0.1 M) or HCl (0.1 M).…”

Section: Methodsmentioning

confidence: 99%

“…We investigated the oxidase-like activity of SeNPs, as previously reported by our group [ 26 ]. Briefly, the catalytic activity (i.e., oxidation of the peroxidase substrate) of SeNPs was observed in 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H 2 O 2, Sigma–Aldrich, USA).…”

Section: Methodsmentioning

confidence: 99%

“…In particular, nanoparticles with diameters of 1–100 nm can modulate cell fate by directly delivering themselves to intracellular sites, such as to the cytosol and nucleus [ 20 , 21 , 22 ]. Recently, metal (gold, silver, selenium, strontium, copper, and ceria)-incorporated nanoparticles showed outstanding biocompatible and antioxidant effects in various areas of tissue regeneration [ 20 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

Lee

Patel

et al. 2021

Antioxidants

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Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H2O2-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H2O2. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H2O2, MyoD, MyoG, α-actinin, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.

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“…Importantly, Bi 2 Se 3 has favorable biocompatibility, greatly facilitating their promising application. Bi 2 Se 3 was reported to be degraded, and Se released from Bi 2 Se 3 played an important role through selenoproteins to protect membranes and tissues [ 16 ], prevent cancers [ 17 , 18 ] and regulate immunity [ 19 ]. It was reported that Bi 2 Se 3 nanoplates were intraperitoneally injected into mice at a high dose of 20 mg/kg and finally, 93% nanoplates were metabolized with few side-effects within the 90-day long-term period [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Photothermal Killing of A549 Cells and Autophagy Induction by Bismuth Selenide Particles

You

Chen

et al. 2021

Materials

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With a highly efficient optical absorption capability, bismuth selenide (Bi2Se3) can be used as an outstanding photothermal agent for anti-tumor treatment and shows promise in the field of nanotechnology-based biomedicine. However, little research has been completed on the relevant mechanism underlying the photothermal killing effect of Bi2Se3. Herein, the photothermal effects of Bi2Se3 particles on A549 cells were explored with emphasis put on autophagy. First, we characterized the structure and physicochemical property of the synthesized Bi2Se3 and confirmed their excellent photothermal conversion efficiency (35.72%), photostability, biocompatibility and ability of photothermal killing on A549 cells. Enhanced autophagy was detected in Bi2Se3-exposed cells under an 808 nm laser. Consistently, an elevated expression ratio of microtubule-associated protein 1 light chain 3-II (LC3-II) to LC3-I, a marker of autophagy occurrence, was induced in Bi2Se3-exposed cells upon near infrared (NIR) irradiation. Meanwhile, the expression of cleaved-PARP was increased in the irradiated cells dependently on the exposure concentrations of Bi2Se3 particles. Pharmacological inhibition of autophagy by 3-methyladenine (3-MA) further strengthened the photothermal killing effect of Bi2Se3. Meanwhile, stress-related signaling pathways, including p38 and stress activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), were activated, coupled with the attenuated PI3K/Akt signaling. Our study finds that autophagy and the activation of stress-related signaling pathways are involved in the photothermal killing of cancerous cells by Bi2Se3, which provides a more understanding of photothermal materials.

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The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells

Cited by 21 publications

References 69 publications

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

Photothermal Killing of A549 Cells and Autophagy Induction by Bismuth Selenide Particles

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