The effect of Sebastiania hispida gel on wound model infected by methicillin resistant Staphylococcus aureus

Muller, Jéssica de Araújo Isaías; Matias, Rosemary; Guilhermino, Jislaine de Fátima; Moreira, Davyson de Lima; Santos, Karen Silva dos; Fermiano, Maria Helena; Silva, Baldomero Antonio Kato; Dourado, Doroty Mesquita

doi:10.1016/j.biopha.2018.06.100

Cited by 4 publications

(7 citation statements)

References 27 publications

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“…1A). These results have great similarity in terms of the chemical constituent to the specimens of S. hispida collected in other regions of the Brazilian Cerrado [24,25], except for the saponins, anthraquinones, steroids and reducing sugars (Fig. 1A).…”

Section: Phytochemical Analysis Of S Hispida Methanolic (Meoh) and Hydro-methanolic (Hydro-meoh) Extractssupporting

confidence: 67%

“…Thus, because of the high concentration of polyphenols (phenolic and flavonoids compounds) and greater polarity, the S. hispida hydro-MeOH extract was selected for the treatment of B. moojeni-induced edema and myonecrosis in rats' gastrocnemius, which proved to be a promising candidate for the development of water-soluble healing drugs. In agreement, it was recently described that ethanol-based extract (EtOH-based) hydrogel formulation of S. hispida sprouts used as a complementary therapy for the closure of wounds infected with Staphylococcus aureus [25].…”

Section: Phytochemical Analysis Of S Hispida Methanolic (Meoh) and Hydro-methanolic (Hydro-meoh) Extractsmentioning

confidence: 70%

“…On the other hand, the chemical arsenal of constituents in plants makes important therapeutic allies to neutralize snake venom effects [69][70][71][72]. In this sense, S. hispida richness in flavonoids and phenolic compounds showed wound healing activity [24], likely based on its antibacterial activity [25]. In the present study model of B. moojeni-induced myonecrosis the partial but effective efficacy of S. hispida hydro-MeOH extract might rely on secondary metabolites, flavonoids and tannins, which demonstrated their anti-inflammatory and antioxidant properties [73,74].…”

Section: Histopathologic Datamentioning

confidence: 99%

“…Sebastiania hispida (Mart.) Pax (Euphorbiaceae) is a native species of the Cerrado bioma characteristic of semi-flooded fields at the Brazilian Pantanal and that has been described as possessing a significant anti-inflammatory potential [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Benefits of Sebastiania hispida (Euphorbiaceae) extract and photobiomodulation therapy as potentially adjunctive strategies to be explored against snake envenoming

Dourado

Matias

Silva³

et al. 2021

Photochem Photobiol Sci

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The purpose of this study was to assess the topic use of Sebastiania hispida extract and low-level gallium-arsenide laser irradiation (GaAs, 904 nm) to reduce the local myonecrosis and edema of Bothrops moojeni snake venom-injected gastrocnemius. Wistar rats receiving intramuscular venom injection (VBm) were compared with saline control (S) and envenomed rats receiving local exposure to plant extract (VExt) or laser irradiation (VL). The phytochemistry and thin-layer chromatography of S. hispida extract indicated the presence of phenolic compounds like gallic acid and flavonoids including quercetin. Gastrocnemius of VExt and VL groups had a significant reduction of edema and creatine kinase (CK) activities and a greater Myogenin (MyoG) expression compared to VBm group, with the plant extract efficacy better than laser exposure. Reduction of edema and serum CK activities reflects a lessening of muscle damage, whereas the increase of MyoG indicates myoblast differentiation and acceleration of muscle repair. The S. hispida richness in phenolic compounds and flavonoids, such as the light modulatory ability to triggering a multitude of cell signalings likely underlie the positive outcomes. Our findings suggest both treatments as potential auxiliary tools to be explored in clinical trials in combination with anti-venom therapy after Bothropic snakebites.

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Section: Phytochemical Analysis Of S Hispida Methanolic (Meoh) and Hydro-methanolic (Hydro-meoh) Extractssupporting

confidence: 67%

Section: Phytochemical Analysis Of S Hispida Methanolic (Meoh) and Hydro-methanolic (Hydro-meoh) Extractsmentioning

confidence: 70%

Section: Histopathologic Datamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Benefits of Sebastiania hispida (Euphorbiaceae) extract and photobiomodulation therapy as potentially adjunctive strategies to be explored against snake envenoming

Dourado

Matias

Silva³

et al. 2021

Photochem Photobiol Sci

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“…However, this has been losing its strength due to increasing bacterial resistance and superinfections. Thus, studies on phytotherapy, in an attempt to aid healing and contain infections in cutaneous wounds (Haddad et al 2000;Muller et al 2018).…”

Section: Evaluation Of the Scientific Activity Of The Leaf Of The Species Campomanesia Pubescens In Wound Model Infected By Staphylococcumentioning

confidence: 99%

Evaluation of the scientific activity of the leaf of the species Campomanesia pubescens in wound model infected by Staphylococcus aureus

et al. 2021

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There is an increasing interest in the use of herbal remedies as healing agents, due to their lower cost in relation to other drugs and the vast Brazilian fauna. The objective of this research was to evaluate the cicatrization effect of the guariroba leaf (Campomanesia pubescens) on the healing of infected wounds. We used 45 Wistar rats, distributed in five groups (n = 9) all with surgically induced skin injury, differing in the presence of contamination and treatment, with evaluation periods of 3, 7 and 14 days, being: G1- negative control without contamination, treated with Physiological Solution 0.9%; G2- control with contamination, treated with Physiological Solution 0.9%; G3 negative with contamination, treated with Carbopol in 0.5% gel; G4- positive control with contamination, treated with Colagenase at 0.6 U / g + 0.01 g Chloramphenicol; G5- positive test with contamination treated with Campomanesia pubescens at 3%, whose vehicle was Carbopol at 0.5%. The wound was made with a metal punch 8 mm in diameter, and a cutaneous fragment was removed from the animals' backs and wound infection was applied to S. aureus in groups G2 to G5. Euthanasia was performed for a lethal dose of anesthetic, and the edges of the wounds were removed for histopathological study. The fibrinoleukocytic crust was present in all animals in the groups of 3, 7 and 14 days. The contraction of the wound was also evaluated, and all groups showed low percentage of wound regression in the 3-day treatment and with 14 days presented a high percentage of regression. Of the 5 groups, the only one that presented complete epithelialization was G5. Of the 5 groups, the ones with the best epithelialization were G4 and G5. The group with the highest amount of mature collagen fibers was G4, followed by G5, and the one with the highest proportion of immature fibers was G1. At the end of the experiment, G4 was the group that gained the most weight and G1 the one that had the lowest weight gain. Guariroba leaf extract (Campomanesia pubescens) was able to promote healing in infected skin wounds similar to the group treated with antibiotics.

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Avaliação do Efeito Neuroprotetivo de Sebastiania hispida (Mart.) Pax em um Modelo de Neuropatia Periférica em Camundongos

Silva

Fontoura²,

Santos³

et al. 2022

Ensaios Ciênc.

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A neuropatia periférica é o resultado de diversas patologias, entre essas se destacando a diabetes, as doenças autoimunes e intoxicações por metais pesados como chumbo e agrotóxicos, existentes na sociedade contemporânea, e em virtude de tal fato, o estudo para a prevenção e tratamento da mesma se faz necessário. O propósito do estudo foi comparar fatores clínicos, morfológicos, bioquímicos e inflamatórios entre diferentes grupos experimentais de camundongos com neuropatia periférica induzida pela compressão do nervo ciático e grupo controle. O estudo busca minimizar seus efeitos por meio do uso do extrato à base da planta Sebastiana hispida, comparando a mesma com os resultados do tratamento convencional com a gabapentina, um anticonvulsivante com o maior número de evidências científicas para dor neuropática, porém, de alto custo.Trata-se de um estudo randomizado com 40 camundongos isogênicos Swiss, divididos em 8 grupos com 5 indivíduos em cada, sendo esses: Sham (G1), Controle (G2), Extrato à base de S. hispida via tópica (G3) e Gabapentina via oral (G4), sendo que estes 4 grupos foram observados em um período de 7 dias e outros 4 grupos em 14 dias. Neste estudo, avaliou-se a fitoquímica da S. hispida, evidenciando uma concentração elevada de flavonoides, que demonstrou sua eficácia neuroprotetiva, na avaliação funcional do nervo ciático, comparado com a histopatologia. Concluiu-se que o tratamento tópico com o extrato à base de Sebastiana hispida contribuiu para a recuperação funcional do nervo ciático, além da regressão da ferida e da cicatrização do nervo ciático. Palavras-chaves: Dor Neuropática. Nervo Ciático. Tratamento com Plantas Medicinais. Flavonoides. Abstract Peripheral neuropathy is the result of various pathologies existing in contemporary society, and because of this fact, the study for its prevention and treatment is necessary. The purpose of the study was to compare clinical, morphological, biochemical and inflammatory factors among different groups of mice with peripheral neuropathy, induced by sciatic nerve compression and without peripheral neuropathy. The study seeks to minimize its effects through the use of Sebastiana hispida plant extract, comparing it with the results of conventional treatment with gabapentin, an anticonvulsant with the largest amount of scientific evidence for neuropathic pain, but costly. This is a randomized study of 40 isogenic Swiss mice, divided into 8 groups with 5 individuals each: Sham (G1), Control (G2), S. hispida Extract based on topical (G3) and Gabapentin via (G4), with 4 groups being observed within seven days and 4 groups within 14 days. In this study, the phytochemistry of Sebastiana hispida was evaluated, showing a high concentration of flavonoids, which demonstrated its neuroprotective efficacy in the functional evaluation of the sciatic nerve compared with histopathology. It was concluded that topical treatment with S. hispida extract contributed to the functional recovery of the sciatic nerve, as well as wound regression and sciatic nerve healing. Keywords: Neuropathic Pain, Sciatic Nerve. Herbal Medicine. Treatment with Medicinal Plants. Flavonoids.

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The effect of Sebastiania hispida gel on wound model infected by methicillin resistant Staphylococcus aureus

Cited by 4 publications

References 27 publications

Benefits of Sebastiania hispida (Euphorbiaceae) extract and photobiomodulation therapy as potentially adjunctive strategies to be explored against snake envenoming

Benefits of Sebastiania hispida (Euphorbiaceae) extract and photobiomodulation therapy as potentially adjunctive strategies to be explored against snake envenoming

Evaluation of the scientific activity of the leaf of the species Campomanesia pubescens in wound model infected by Staphylococcus aureus

Avaliação do Efeito Neuroprotetivo de Sebastiania hispida (Mart.) Pax em um Modelo de Neuropatia Periférica em Camundongos

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