The effect of resveratrol on the recurrent attacks of gouty arthritis

Chen, Haiyan; Zheng, Shucong; Wang, Yuan-Kai; Zhu, Huan; Liu, Qiong; Yu, Xue; Qiu, Jianhua; Zou, Hejian; Zhu, Xiaoxia

doi:10.1007/s10067-014-2836-3

Cited by 49 publications

(25 citation statements)

References 25 publications

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“…To our surprise, IL-37 was a potent anti-inflammatory agent, even given at 4 h after MSU crystal administration (Fig. 4g), at which time point the inflammatory cascade was shown to have started in the mouse gouty arthritis model in our previous study [16]. …”

Section: Resultsmentioning

confidence: 89%

Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout

Liu

Zhu

et al. 2016

Arthritis Res Ther

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BackgroundInterleukin (IL)-37 has emerged as a fundamental inhibitor of innate immunity. Acute gout is a self-limiting inflammatory response to monosodium urate (MSU) crystals. In the current study, we assessed the preventive and therapeutic effect of recombinant human IL-37 (rhIL-37) in human and murine gout models.MethodsWe investigated the expression of IL-37 in patients with active and inactive gouty arthritis and assessed the effect of rhIL-37 in human and murine gout models: a human monocyte cell line (THP-1) and human synovial cells (containing macrophage-like and fibroblast-like synoviocytes) exposed to MSU crystals, a peritoneal murine model of gout and a murine gouty arthritis model. After inhibition of Mer receptor tyrosine kinase (Mertk), levels of IL-1β, IL-8 and chemokine (C-C motif) ligand 2 (CCL-2) were detected by ELISA and expression of mammalian homologs of the drosophila Mad gene 3 (Smad), suppressor of cytokine signaling 3 (SOCS3), NACHT-LRR-PYD-containing protein 3 (NLRP3), and IL-8R of THP-1 were assessed by qPCR and western blot to explore the molecular mechanisms.ResultsOur studies strongly indicated that rhIL-37 played a potent immunosuppressive role in the pathogenesis of experimental gout models both in vitro and in vivo, by downregulating proinflammatory cytokines and chemokines, markedly reducing neutrophil and monocyte recruitment, and mitigating pathological joint inflammation. In our studies, rhIL-37 suppressed MSU-induced innate immune responses by enhancing expression of Smad3 and IL-1R8 to trigger multiple intracellular switches to block inflammation, including inhibition of NLRP3 and activation of SOCS3. Mertk signaling participated in rhIL-37 inhibitory pathways in gout models. By inhibition of Mertk, the anti-inflammatory effect of rhIL-37 was partly abrogated, and IL-1R8, Smad3 and SOCS3 expression were suppressed, whereas NLRP3 expression was reactivated.ConclusionsOur studies reveal that IL-37 limits runaway inflammation initiated by MSU crystal-induced immune responses, partly in a Mertk-dependent fashion. Thus, rhIL-37 has both preventive and therapeutic effects in gouty arthritis.

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Section: Resultsmentioning

confidence: 89%

Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout

Liu

Zhu

et al. 2016

Arthritis Res Ther

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“…We next tried to better understand whether miR-155 deficiency affects the inflammatory response in MSU-induced gouty inflammation in vivo . According to a description of murine gout models [ 14 – 16 ], the manifestation of inflammation such as redness and a swollen joint, and the swelling index were used to evaluate the inflammatory levels. We used MSU-induced foot pad inflammation and MSU-induced ankle arthritis models.…”

Section: Resultsmentioning

confidence: 99%

“…Inflammation parameters were evaluated following MSU crystal injection at different time points (6, 24, and 48 h). Paw swelling and the size of ankle joints were measured with an electronic caliper at the indicated time points [ 14 – 16 ]. For an air pouch model, 5 ml sterile air was first injected subcutaneously into the back of mice to form an air pouch, and then 3 ml sterile air was injected to the air pouch at day 3 and day 5.…”

Section: Methodsmentioning

confidence: 99%

miR-155 is dispensable in monosodium urate-induced gouty inflammation in mice

et al. 2018

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BackgroundThe findings of a previous study by Jin et al. have shown that microRNA (miR)-155 was upregulated in patients with acute gouty arthritis and enhanced the proinflammatory cytokines. There is no direct evidence to support that miR-155 is indeed involved in monosodium urate (MSU)-induced inflammatory responses in vivo. The aim of this study was to investigate the role of miR-155 knock-out (KO) or knock-in (KI) mice in MSU-induced animal models to mimic acute gout.MethodsMiR-155 expression in cultured bone marrow-derived macrophages (BMDMs) from miR-155 KO, miR-155 KI, and wild-type (WT) mice treated with MSU crystals in vitro was detected by real-time quantitative polymerase chain reaction (qPCR). MiR-155 KO and WT mice were used to induce an acute gouty inflammatory response with MSU crystals including models of foot pad inflammation, ankle arthritis, air pouch inflammation, and peritonitis. Furthermore, the proinflammatory interleukin (IL)-1β levels in lavage fluids from air pouch and peritoneal cavity models were measured by enzyme-linked immunosorbent assay (ELISA), and tumor necrosis factor (TNF)-α production from BMDMs of miR-155 KI mice treated with MSU were measured by flow cytometry.ResultsMiR-155 expression was quickly upregulated in BMDMs from WT mice following MSU treatment in vitro. In comparison with WT mice in vivo, the swelling index of miR-155 KO mice showed no significant difference in the murine foot pad and ankle arthritis models for the indicated different time points. There were similar changes in total cell numbers of lavage fluids in the air pouch and peritoneal cavity models between miR-155 KO and WT mice following MSU crystal injection. Moreover, the IL-1β levels of lavage fluids in the air pouch and peritonitis models from miR-155 KO mice were almost the same as those from WT mice. TNF-α levels were comparable from BMDMs treated with MSU crystals in vitro between miR-155 KI mice and WT mice.ConclusionsMiR-155 is dispensable in MSU-induced gouty inflammation in mice. Deletion of miR-155 might not be an effective therapeutic approach to relieve the inflammation in acute gout.

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“…The sole use of an inhibitor as a model agent cannot completely simulate high purine uptake and induce a consistently high UA levels in mice. [31,32] Therefore, in this study, we induced hyperuricaemia in mice by administering the precursor of UA, hypoxanthine, together with potassium oxonate.…”

Section: Discussionmentioning

confidence: 99%

Erding Formula in hyperuricaemia treatment: unfolding traditional Chinese herbal compatibility using modern pharmaceutical approaches

Zuo

Bou‐Chacra

et al. 2017

Journal of Pharmacy and Pharmacology

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Objectives Traditional Chinese herbal formulas are difficult to be understood because of complex compositions and specific therapeutic principles. To better understand herbal compatibility in Traditional Chinese medicine (TCM), this study was conducted to investigate the effects of a Chinese pharmacopoeia-listed formula, Erding Formula (EF) and its constituent herbs for a new indication, hyperuricaemia. Methods A hypoxanthine and potassium oxonate-induced hyperuricemic mouse model, a xylene-induced inflammatory mouse model and an acetic acid-induced pain model were used to test the effects of EF and its constituent herbs. In addition, we investigated whether EF and/or its relevant herbs had an impact on the expression of URAT1 and OAT3 mRNA. Key findings The results showed EF and individual herbs had pharmacological effects on selected targets. Only Viola yedoensis Makino (Viola) lowered uric acid levels, while all four herbs had anti-inflammatory and analgesic effects. The EF may lower the uric acid level through inhibiting the expression of URAT1 mRNA and enhancing the expression of OAT3 mRNA. Conclusions These findings provide pharmacological insights into the effects of EF and individual herbs on UA excretion. This study suggests that Viola is the main herb in EF. This study facilitates better understanding of TCM principles and theories using modern pharmaceutical approaches.

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The effect of resveratrol on the recurrent attacks of gouty arthritis

Cited by 49 publications

References 25 publications

Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout

Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout

miR-155 is dispensable in monosodium urate-induced gouty inflammation in mice

Erding Formula in hyperuricaemia treatment: unfolding traditional Chinese herbal compatibility using modern pharmaceutical approaches

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